Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,244,739	A→G	S113P (TCC→CCC)	malF ←	maltose transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,244,739	0	A	G	100.0%	136.6 / NA	38	S113P (TCC→CCC)	malF	maltose transporter subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/21); total (17/21)

CAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGTGCTGCTGTAGTTGGTGAAGGCAATGGCGATGGTGCAGACCAGAGGGAAGAGGACGAATAATCCCATTCCAGCCAT  >  NC_000913/4244605‑4244862

cAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGa                                                                                                                         <  2:785569/139‑1 (MQ=255)

cAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGa                                                                                                                         <  1:1218374/139‑1 (MQ=255)

cAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGa                                                                                                                         >  1:635978/1‑139 (MQ=255)

        gCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTaacaa                                                                                                                 <  2:591482/139‑1 (MQ=255)

                               tCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTaacaa                                                                                                                 <  2:21509/116‑1 (MQ=255)

                               tCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTaacaa                                                                                                                 >  1:21509/1‑116 (MQ=255)

                               tCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAg                                                                                          >  2:1120005/1‑139 (MQ=255)

                                   agagGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAg                                                                                      <  1:1163562/139‑1 (MQ=255)

                                   agagGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAg                                                                                      <  2:1172563/139‑1 (MQ=255)

                                           tttttGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAg                                                                              <  1:985260/139‑1 (MQ=255)

                                            ttttGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGt                                                                             <  2:635978/139‑1 (MQ=255)

                                                 ccGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGtgctgc                                                                        >  1:428822/1‑139 (MQ=255)

                                                  cGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGTgctgct                                                                       >  2:814961/1‑139 (MQ=255)

                                                      ttCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTTAAAAGTCAGCTGGTTAGTGCTGCTGTAg                                                                   <  1:814961/139‑1 (MQ=255)

                                                               gCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGTgctgct                                                                       >  1:1144519/1‑126 (MQ=255)

                                                               gCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGTgctgct                                                                       <  2:1144519/126‑1 (MQ=255)

                                                                   aGCGCCAGTAGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGTGCTGCTGTAGTTGGTGAAGGCaa                                                      >  2:629596/1‑139 (MQ=255)

                                                                         aGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGTGCTGCTGTAGTTGGTGAAGGCAATGGCGa                                                <  1:468170/139‑1 (MQ=255)

                                                                             gCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCC‑‑‑‑TGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAg                                                                                      >  2:1210765/1‑93 (MQ=255)

                                                                             gCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCC‑‑‑‑TGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAg                                                                                      <  1:1210765/93‑1 (MQ=255)

                                                                              ccACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGTGCTGCTGTAGTTGGTGAAGGCAATGGCGATGGTg                                           <  1:629596/139‑1 (MQ=255)

                                                                                     tCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGTGCTGCTGTAGTTGGTGAAGGCAATGGCGATGGTGCAGACCa                                    <  2:428822/139‑1 (MQ=255)

                                                                                      cgcccgccGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGt                                                                             >  2:1152714/1‑97 (MQ=255)

                                                                                      cgcccgccGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGt                                                                             <  1:1152714/97‑1 (MQ=255)

                                                                                      cgcccgccGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGTGCTGCTGTAGTTGGTGAAGGCAATGGCGATGGTGCAGACCag                                   >  1:101496/1‑139 (MQ=255)

                                                                                                gTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAg                                                                              <  2:131888/86‑1 (MQ=255)

                                                                                                gTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAg                                                                              >  1:131888/1‑86 (MQ=255)

                                                                                                                     tttGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAg                                                                              <  1:673238/65‑1 (MQ=255)

                                                                                                                     tttGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAg                                                                              <  2:714735/65‑1 (MQ=255)

                                                                                                                     tttGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAg                                                                              >  2:673238/1‑65 (MQ=255)

                                                                                                                     tttGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAg                                                                              >  1:714735/1‑65 (MQ=255)

                                                                                                                       tGCCTGCTTGCCAGGGGCGATCTAACAATACTTCCTGCGCACGTTCAAAAGTCAGATGGTTAGTGCTGCTGTAGTTGGTGAAGGCAATGGCGATGGTGCa                                         >  2:1190133/1‑100 (MQ=255)

                                                                                                                       tGCCTGCTTGCCAGGGGCGATCTAACAATACTTCCTGCGCACGTTCAAAAGTCAGATGGTTAGTGCTGCTGTAGTTGGTGAAGGCAATGGCGATGGTGCa                                         <  1:1190133/100‑1 (MQ=255)

                                                                                                                       tGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGt                                                                             <  2:324842/64‑1 (MQ=255)

                                                                                                                       tGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGt                                                                             >  1:324842/1‑64 (MQ=255)

                                                                                                                       tGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGTGCTGCTGTAg                                                                   <  2:1286349/74‑1 (MQ=255)

                                                                                                                       tGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGTGCTGCTGTAg                                                                   >  1:1286349/1‑74 (MQ=255)

                                                                                                                       tGCCTGCTTGCCAGGGGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGTGCTGCTGTAGTTGGTGAAGGCAATGGCGATGGTGCAGACCAGAGGGAAGAGGACGAATAATCCCATTCCAGCCAt  >  1:770994/1‑139 (MQ=255)

CAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGTCAGCTGGTTAGTGCTGCTGTAGTTGGTGAAGGCAATGGCGATGGTGCAGACCAGAGGGAAGAGGACGAATAATCCCATTCCAGCCAT  >  NC_000913/4244605‑4244862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A