Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	778	68.7	3228504	93.4%	3015422	109.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,207,942	(G)_7→8	coding (442/630 nt)	stfP →	e14 prophage, uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,207,935	1	.	G	96.5%	290.7 / 1.7	86	coding (435/630 nt)	stfP	e14 prophage, uncharacterized protein
Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (42/41); total (43/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 104 total aligned reads displayed.

GGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTT  >  NC_000913/1207797‑1208066

GGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCc‑tc‑t                                                                                                                                        <  1:1427881‑M1/139‑1 (MQ=255)

     AAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑ggggg                                                                                                                                 >  1:67438‑M1/1‑139 (MQ=255)

      AGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg                                                                                                                                <  2:955312‑M1/139‑1 (MQ=255)

           cAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑gggat                                                                                                                          >  2:908002/1‑135 (MQ=255)

               aCTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGaaa                                                                                                                       >  1:1198617/1‑139 (MQ=255)

                  cAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TGGGGGGGG‑GATGGAAATa                                                                                                                     <  1:43785/139‑1 (MQ=255)

                          caACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTAt                                                                                                            >  2:317094/1‑139 (MQ=255)

                          caACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTAt                                                                                                            >  2:1502387/1‑139 (MQ=255)

                               gCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGTTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑GGGGGGCGATGGAAATACAGTTGTATTCCCg                                                                                                       >  1:819443/1‑139 (MQ=255)

                                 tACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑gga                                                                                                                            <  2:301730/115‑3 (MQ=255)

                                  aCATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGATGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGtt                                                                                                    <  2:1196807/139‑1 (MQ=255)

                                   cATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTg                                                                                                   >  1:1437452/1‑139 (MQ=255)

                                        ggACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑g                                                                                                                              <  2:815024/106‑1 (MQ=255)

                                        ggACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑g                                                                                                                              >  1:815024/1‑106 (MQ=255)

                                             cACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTtaataa                                                                                         >  2:937780/1‑139 (MQ=255)

                                             cACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTtaataa                                                                                         >  1:1183944/1‑139 (MQ=255)

                                             cACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCAGGTTGCGTTtaataa                                                                                         >  1:7586/1‑139 (MQ=255)

                                              aCCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTaataat                                                                                        <  2:7586/139‑1 (MQ=255)

                                                cGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGc                                                                                      <  2:1605088/139‑1 (MQ=255)

                                                             ataCCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgtt                                                                         >  1:219978/1‑139 (MQ=255)

                                                              taCCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgttg                                                                        >  2:642432/1‑139 (MQ=255)

                                                              taCCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑g                                                                                                                              >  2:431128/1‑84 (MQ=255)

                                                              taCCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑g                                                                                                                              <  1:431128/84‑1 (MQ=255)

                                                                 cGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                                                     <  2:1427881/139‑1 (MQ=255)

                                                                 cGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                                                     <  2:1198617/139‑1 (MQ=255)

                                                                 cGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                                                     <  2:1183944/139‑1 (MQ=255)

                                                                   gTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTaataat                                                                                        >  1:254098/1‑118 (MQ=255)

                                                                     tATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATa                                                                                                                     <  2:458124/87‑1 (MQ=255)

                                                                     tATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATa                                                                                                                     >  1:458124/1‑87 (MQ=255)

                                                                             gtggtATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATACTGCCTGTGTTGCCGTTGTTGCAAATTATGACa                                                         >  2:1452868/1‑139 (MQ=255)

                                                                             gtggtATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACa                                                         >  2:829573/1‑139 (MQ=255)

                                                                             gtggtATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACa                                                         >  2:189157/1‑139 (MQ=255)

                                                                                 tATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGt                                                                                                >  2:149249/1‑96 (MQ=255)

                                                                                 tATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGt                                                                                                <  1:149249/96‑1 (MQ=255)

                                                                                       gCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgcgc                                               >  2:43967/1‑139 (MQ=255)

                                                                                        cAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGGCAATGTCAgcg                                                <  2:1240907/137‑1 (MQ=255)

                                                                                        cAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGGCAATGTCAgcg                                                >  1:1240907/1‑137 (MQ=255)

                                                                                        cAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCa                                              <  1:189157/139‑1 (MQ=255)

                                                                                        cAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCa                                              <  1:970179/139‑1 (MQ=255)

                                                                                           tCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGt                                                                                                <  1:1193584/86‑1 (MQ=255)

                                                                                           tCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGt                                                                                                >  2:1193584/1‑86 (MQ=255)

                                                                                           tCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCt                                           >  1:292723/1‑139 (MQ=255)

                                                                                             aGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑ggat                                                                                                                           >  2:195851/1‑53 (MQ=255)

                                                                                             aGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑ggat                                                                                                                           <  1:195851/56‑4 (MQ=255)

                                                                                              gTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATc                                        <  2:292723/139‑1 (MQ=255)

                                                                                                aaTCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                                                     >  2:1463027/1‑108 (MQ=255)

                                                                                                aaTCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                                                     <  1:1463027/108‑1 (MQ=255)

                                                                                                aaTCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑g                                                                                                                              >  1:431788/1‑50 (MQ=255)

                                                                                                aaTCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑g                                                                                                                              <  2:431788/50‑1 (MQ=255)

                                                                                                  tCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑g                                                                                                                              >  2:1162702/1‑48 (MQ=255)

                                                                                                  tCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑g                                                                                                                              <  1:1162702/48‑1 (MQ=255)

                                                                                                     ttCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCg                                                                           <  1:1601245/97‑1 (MQ=255)

                                                                                                     ttCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCg                                                                           >  2:1601245/1‑97 (MQ=255)

                                                                                                     ttCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                                                     <  2:296783/103‑1 (MQ=255)

                                                                                                     ttCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                                                     >  1:296783/1‑103 (MQ=255)

                                                                                                     ttCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCa                                                                   <  1:1444307/105‑1 (MQ=255)

                                                                                                     ttCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCa                                                                   >  2:1444307/1‑105 (MQ=255)

                                                                                                       cAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑gga                                                                                                                            >  2:676120/1‑43 (MQ=255)

                                                                                                       cAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑gggggg‑gga                                                                                                                            <  1:676120/45‑3 (MQ=255)

                                                                                                         gTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TGGGGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTAt                                         >  2:837561/1‑128 (MQ=255)

                                                                                                         gTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TGGGGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTAt                                         <  1:837561/128‑1 (MQ=255)

                                                                                                         gTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAATGGc                             <  1:937780/139‑1 (MQ=255)

                                                                                                         gTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAG‑TGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCa                            >  2:895776/1‑139 (MQ=255)

                                                                                                          tGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATAc                                                                                                                    >  1:24056/1‑51 (MQ=255)

                                                                                                          tGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATAc                                                                                                                    <  2:24056/51‑1 (MQ=255)

                                                                                                           gggggTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCaa                           <  2:219978/139‑1 (MQ=255)

                                                                                                            ggggTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGt                                <  2:1349898/133‑1 (MQ=255)

                                                                                                            ggggTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGt                                >  1:1349898/1‑133 (MQ=255)

                                                                                                             gggTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                                                     <  1:277466/95‑1 (MQ=255)

                                                                                                             gggTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                                                     >  2:277466/1‑95 (MQ=255)

                                                                                                             gggTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACg                         >  2:3733/1‑139 (MQ=255)

                                                                                                             gggTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACg                         <  1:642432/139‑1 (MQ=255)

                                                                                                              ggTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACgg                        <  2:1183391/139‑1 (MQ=255)

                                                                                                              ggTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACgg                        <  1:829573/139‑1 (MQ=255)

                                                                                                               gTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACggg                       >  1:1128834/1‑139 (MQ=255)

                                                                                                               gTGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACggg                       >  2:1607243/1‑139 (MQ=255)

                                                                                                                tGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGtt                                                                                                    <  1:657084/61‑1 (MQ=255)

                                                                                                                tGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGtt                                                                                                    >  2:657084/1‑61 (MQ=255)

                                                                                                                tGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACgggg                      <  1:43967/139‑1 (MQ=255)

                                                                                                                tGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACgggg                      <  1:317094/139‑1 (MQ=255)

                                                                                                                tGGAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACgggg                      <  1:1607243/139‑1 (MQ=255)

                                                                                                                 gtaaTGCCGCAAAAGCGTCTGCCGTT‑G‑‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACggggg                     <  2:1437452/137‑1 (MQ=255)

                                                                                                                  gAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACgggggg                    >  1:959375/1‑139 (MQ=255)

                                                                                                                  gAATGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACgggggg                    >  1:707387/1‑139 (MQ=255)

                                                                                                                   aaTGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGa                   <  2:809758/139‑1 (MQ=255)

                                                                                                                    aTGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGc                                                                                      >  2:59695/1‑71 (MQ=255)

                                                                                                                    aTGCCGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGc                                                                                      <  1:59695/71‑1 (MQ=255)

                                                                                                                       ccGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCa                                                   <  1:568186/103‑1 (MQ=255)

                                                                                                                       ccGCAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCa                                                   >  2:568186/1‑103 (MQ=255)

                                                                                                                        cGCAAAAGCATCTGCC‑TC‑TGGGGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCa                                              >  1:1603639/1‑108 (MQ=255)

                                                                                                                        cGCAAAAGCATCTGCC‑TC‑TGGGGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCa                                              <  2:1603639/108‑1 (MQ=255)

                                                                                                                          cAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCa                                              >  1:1106177/1‑105 (MQ=255)

                                                                                                                          cAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACggggg                     >  2:1370141/1‑130 (MQ=255)

                                                                                                                          cAAAAGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACggggg                     <  1:1370141/130‑1 (MQ=255)

                                                                                                                            aaaGCATCTGCC‑TCGG‑‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGGTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAAcc          <  1:955312/139‑1 (MQ=255)

                                                                                                                             aaGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCa         >  2:1255908/1‑139 (MQ=255)

                                                                                                                              aGCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAG‑TGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACt       <  1:895776/139‑1 (MQ=255)

                                                                                                                               gCATCTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACt       <  1:3733/139‑1 (MQ=255)

                                                                                                                                   cTGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGt   <  2:707387/139‑1 (MQ=255)

                                                                                                                                    tGCC‑TC‑TG‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGtt  >  1:980116/1‑139 (MQ=255)

GGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCC‑TC‑T‑‑GGGGGG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTGGCAACGGGGGGATATACAACCACTTCGTT  >  NC_000913/1207797‑1208066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A