Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A13 F1 I1 R1 | 778 | 68.7 | 3228504 | 93.4% | 3015422 | 109.7 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC JC | NC_000913 | 1,426,394 | IS5 (–) +4 bp | intergenic (+312/‑57) | ydaY → / → tmpR | pseudogene, Rac prophage,Phage or Prophage Related/Rac prophage, pseudogene, tail protein family,Phage or Prophage Related, putative alpha helix protein |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 1396044 = | NA (NA) | 71 (1.020) | 46/218 | 0.3 | 100% | noncoding (1/1195 nt) | IS5 | repeat region |
| ? | NC_000913 | 1426394 = | 0 (0.000) | intergenic (+312/‑60) | ydaY/tmpR | pseudogene, Rac prophage,Phage or Prophage Related/Rac prophage, pseudogene, tail protein family,Phage or Prophage Related, putative alpha helix protein | |||||
| * | ? | NC_000913 | = 1397238 | NA (NA) | 74 (1.070) | 44/218 | 0.3 | 100% | noncoding (1195/1195 nt) | IS5 | repeat region |
| ? | NC_000913 | = 1426397 | 0 (0.000) | intergenic (+315/‑57) | ydaY/tmpR | pseudogene, Rac prophage,Phage or Prophage Related/Rac prophage, pseudogene, tail protein family,Phage or Prophage Related, putative alpha helix protein | |||||
TCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < NC_000913/1396178‑1396044‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGAC > NC_000913/1426394‑1426527 TCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAT > 1:1256294/1‑139 GTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTG > 1:234048/1‑139 TGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAA > 2:85610/1‑139 TACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGC > 1:150442/1‑139 TGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGG > 2:113745/1‑139 AATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTT < 1:432901/139‑1 CACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTT < 2:609644/139‑1 ACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCG > 2:723293/1‑127 ACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCG < 1:723293/127‑1 TGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTT > 1:640756/1‑139 CGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCt < 2:990165/122‑2 CGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTC < 2:1593550/139‑1 CGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCG > 1:990165/1‑122 GGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCA < 2:133642/139‑1 CGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGG < 2:640756/139‑1 AAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAG < 2:191771/139‑1 GACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGG < 2:234048/139‑1 GATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCA < 2:1394835/139‑1 ACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCT > 1:716287/1‑139 CCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTC > 1:1196281/1‑139 ATGGCTCCAGATGACTAACATGATTTCATATCAGGGACTTGTTTGTACCTTCCCTAGTTTCTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGC < 1:1537883/139‑1 ATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGC < 2:1196281/139‑1 TGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGG < 2:1435764/82‑1 TGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGG > 1:1435764/1‑82 GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAA < 1:973809/139‑1 GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAA < 2:1535902/139‑1 CCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAAT > 1:1163182/1‑139 CCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAG > 2:477684/1‑95 CCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAG < 1:477684/95‑1 CCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCG > 1:79250/1‑76 CCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCG < 2:79250/76‑1 CAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATT < 2:716287/139‑1 GATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTT < 2:1108183/97‑1 GATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTT > 1:1108183/1‑97 GATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGG < 1:172852/74‑1 GATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGG > 2:172852/1‑74 CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATA > 2:1395395/1‑139 CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATT > 2:709954/1‑132 CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATT < 1:709954/132‑1 AAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAG > 2:73503/1‑89 AAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAG < 1:73503/89‑1 TGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTG < 2:1256294/139‑1 ATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGC > 2:880377/1‑43 ATCTCATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGC < 1:880377/43‑1 ATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCA < 1:1086318/129‑1 ATATCAGGGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCA > 2:1086318/1‑129 GGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAAT > 2:322642/1‑139 GGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCT < 2:1280719/102‑1 GGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTCTTAATAAATGGCAGAGCAAACCTCGCGTCT > 1:1280719/1‑102 GGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAG > 1:61105/1‑67 GGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAG < 2:61105/67‑1 GGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGG < 1:113892/49‑1 GGACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGG > 2:113892/1‑49 GACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGG > 1:718175/1‑48 GACTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGG < 2:718175/48‑1 CTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCT > 2:784998/1‑139 CTTGTTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCT > 1:608095/1‑139 TTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACA > 2:38305/1‑139 TTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACA > 2:882633/1‑139 TTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGG > 2:433109/1‑42 TTCGCACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGG < 1:433109/42‑1 ACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTG > 2:25038/1‑139 ACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGC < 2:1140683/93‑1 ACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTC < 1:1018702/84‑1 ACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTC > 2:1018702/1‑84 ACCTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTGTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGC > 1:1140683/1‑93 CTTCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGAC < 1:882633/139‑1 TCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAA < 1:1518714/94‑1 TCCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAA > 2:1518714/1‑94 CCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAG < 1:62798/50‑1 CCCTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAG > 2:62798/1‑50 TCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < NC_000913/1396178‑1396044‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGAC > NC_000913/1426394‑1426527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |