Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	778	68.7	3228504	93.4%	3015422	109.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,503,125	G→A	223 (TAG→TAA)	ydcL →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,503,125	0	G	A	100.0%	145.4 / NA	46	223 (TAG→TAA)	ydcL	lipoprotein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/20); total (26/20)

GTTATCAAAGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAGTTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCAATGTATTGGCGGATGAC  >  NC_000913/1502991‑1503259

gTTATCAAAGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTcc                                                                                                                                    >  2:746891/1‑139 (MQ=255)

gTTATCAAAGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTcc                                                                                                                                    >  1:227471/1‑139 (MQ=255)

     cAAAGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGAcg                                                                                                                               <  1:129019/139‑1 (MQ=255)

      aaaGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGACTTCCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCAACAATGTTTGACGTTAACAACAAGTAATTCCAGAcgc                                                                                                                              >  1:1411338/1‑139 (MQ=255)

       aaGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGAcgcg                                                                                                                             <  1:1351675/139‑1 (MQ=255)

                     ggCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATgg                                                                                                               <  1:746891/139‑1 (MQ=255)

                     ggCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATgg                                                                                                               <  2:227471/139‑1 (MQ=255)

                                             gAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTaa                                                                                       <  2:1317773/139‑1 (MQ=255)

                                              aaaGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTaaa                                                                                      >  2:1444066/1‑139 (MQ=255)

                                                 gTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATgg                                                                                                               <  2:1228245/111‑1 (MQ=255)

                                                 gTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATgg                                                                                                               >  1:1228245/1‑111 (MQ=255)

                                                 gTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAAcc                                                                                   >  1:520031/1‑139 (MQ=255)

                                                              tttCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAggg                                                                      <  1:1444066/139‑1 (MQ=255)

                                                              tttCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAggg                                                                      <  2:1411338/139‑1 (MQ=255)

                                                               ttCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGc                                                                     >  2:796506/1‑139 (MQ=255)

                                                                      atatTAAACAAGTTATTGATGACATGGCGCCCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGTCATCGTTAGATGGCGCTTTTTATCCGGTg                                                                                               >  1:1152816/1‑106 (MQ=255)

                                                                      atatTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTg                                                                                               <  2:1152816/106‑1 (MQ=255)

                                                                          tAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAg                                                          >  2:653556/1‑139 (MQ=255)

                                                                                 gTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACTCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAGGGCGCGGt                                                   >  2:956666/1‑139 (MQ=255)

                                                                                 gTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGt                                                   >  1:1258115/1‑139 (MQ=255)

                                                                                    aTTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGtttt                                                >  2:241659/1‑139 (MQ=255)

                                                                                        atgaCATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAggg                                                                      <  1:747073/113‑1 (MQ=255)

                                                                                        atgaCATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAggg                                                                      >  2:747073/1‑113 (MQ=255)

                                                                                            cATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGgcgc                                                      >  1:731558/1‑125 (MQ=255)

                                                                                            cATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGgcgc                                                      <  2:731558/125‑1 (MQ=255)

                                                                                            cATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGa                                        >  1:562205/1‑139 (MQ=255)

                                                                                               ggCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCa                                     >  2:766814/1‑139 (MQ=255)

                                                                                                  gACCGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGAGATATAAGGCGCGGTTTTCCACCTGACCAGgt                                  <  1:956666/139‑1 (MQ=255)

                                                                                                     cGATGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGtt                               <  1:1323871/139‑1 (MQ=255)

                                                                                                       aTGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACgcgc                                                                                                                            <  2:1185002/44‑1 (MQ=255)

                                                                                                       aTGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACgcgc                                                                                                                            >  1:1185002/1‑44 (MQ=255)

                                                                                                       aTGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTg                             >  1:186993/1‑139 (MQ=255)

                                                                                                       aTGCCACCATGTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTg                             >  2:157951/1‑139 (MQ=255)

                                                                                                                 gTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAggg                                                                      >  1:1072857/1‑88 (MQ=255)

                                                                                                                 gTTTGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGCAAAACCCCATCCTTCAggg                                                                      <  2:1072857/88‑1 (MQ=255)

                                                                                                                  tttGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGAc                                       <  2:1016727/118‑1 (MQ=255)

                                                                                                                  tttGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGAc                                       >  1:1016727/1‑118 (MQ=255)

                                                                                                                    tGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGgcgc                                                      >  2:1402478/1‑101 (MQ=255)

                                                                                                                    tGACGTTAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGgcgc                                                      <  1:1402478/101‑1 (MQ=255)

                                                                                                                          tAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAggg                                                                      <  1:810176/79‑1 (MQ=255)

                                                                                                                          tAACAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAggg                                                                      >  2:810176/1‑79 (MQ=255)

                                                                                                                           aaCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTg                                                                                               >  1:911937/1‑53 (MQ=255)

                                                                                                                           aaCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTg                                                                                               <  2:911937/53‑1 (MQ=255)

                                                                                                                           aaCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCAATGTATTGGc         >  1:381209/1‑139 (MQ=255)

                                                                                                                            aCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCg                                                                    >  2:391690/1‑79 (MQ=255)

                                                                                                                            aCAAAAAGTAATTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCg                                                                    <  1:391690/79‑1 (MQ=255)

                                                                                                                                  agtaatTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCAATGTATTGGCGGATGAc  <  1:796506/139‑1 (MQ=255)

GTTATCAAAGTCGTTCGTCAGGGCGAAGGTAAAGACCTGAATAACGAAAGTACGCCAATGGCTTTCGAAAATATTAAACAAGTTATTGATGACATGGCGACCGATGCCACCATGTTTGACGTTAACAAAAAGTAGTTCCAGACGCGCCATCGTTAGATGGCGCTTTTTATCCGGTGCGCCGTAAAACCCCATCCTTCAGGGCGGGGATATAAGGCGCGGTTTTCCACCTGACCAGGTGTTTGCTGTTGTTCAATGTATTGGCGGATGAC  >  NC_000913/1502991‑1503259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A