Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F1 I1 R1 778 68.7 3228504 93.4% 3015422 109.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,757,006 G→A A437T (GCT→ACT)  pykF → pyruvate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,757,0060GA100.0% 220.7 / NA 69A437T (GCT→ACT) pykFpyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/35);  total (34/35)

CCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTT  >  NC_000913/1756878‑1757132
                                                                                                                                |                                                                                                                              
ccaccaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCagag                                                                                                                      >  2:1336301/1‑139 (MQ=255)
        cTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTgg                                                                                                              <  2:387889/139‑1 (MQ=255)
           gCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGcac                                                                                                           >  1:233810/1‑139 (MQ=255)
                 accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                     >  1:773895/1‑139 (MQ=255)
                 accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                     >  1:490336/1‑139 (MQ=255)
                 accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                     >  2:134080/1‑139 (MQ=255)
                 accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                     >  1:1503913/1‑139 (MQ=255)
                    accaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTg                                                                                                  >  1:457866/1‑139 (MQ=255)
                    accaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTg                                                                                                  <  2:490336/139‑1 (MQ=255)
                     ccaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGa                                                                                                 >  1:12331/1‑139 (MQ=255)
                        aCGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                              <  2:233810/139‑1 (MQ=255)
                        aCGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                              <  1:67425/139‑1 (MQ=255)
                           aaaaaaCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                              >  1:146339/1‑136 (MQ=255)
                           aaaaaaCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                              <  2:146339/136‑1 (MQ=255)
                                    cTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGt                                                                                  <  2:1182988/139‑1 (MQ=255)
                                          aGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                            <  2:1371220/139‑1 (MQ=255)
                                          aGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                            <  2:457866/139‑1 (MQ=255)
                                           gTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGt                                                                           >  1:527998/1‑139 (MQ=255)
                                                       cAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCg                                                               >  1:220274/1‑139 (MQ=255)
                                                          aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGcaca                                                                                                          <  1:919836/93‑1 (MQ=255)
                                                          aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGcaca                                                                                                          >  2:52677/1‑93 (MQ=255)
                                                          aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGcaca                                                                                                          <  1:52677/93‑1 (MQ=255)
                                                          aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGcaca                                                                                                          >  2:919836/1‑93 (MQ=255)
                                                          aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGa                                                                                                 >  2:1367383/1‑102 (MQ=255)
                                                          aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGa                                                                                                 <  1:1367383/102‑1 (MQ=255)
                                                           ggCGTTGTGCCTCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCg                                                           <  2:1044567/139‑1 (MQ=255)
                                                             cGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGc                                                         <  1:134080/139‑1 (MQ=255)
                                                                  tgCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCactac                                                    >  2:448625/1‑139 (MQ=255)
                                                                   gCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCactact                                                   <  2:527998/139‑1 (MQ=255)
                                                                       cAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                            >  1:821094/1‑110 (MQ=255)
                                                                       cAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                            <  2:821094/110‑1 (MQ=255)
                                                                          cTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCg                                            >  1:573137/1‑139 (MQ=255)
                                                                             gTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCAt                                         <  2:12331/139‑1 (MQ=255)
                                                                                      aTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                              <  1:702167/77‑1 (MQ=255)
                                                                                      aTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                              >  2:702167/1‑77 (MQ=255)
                                                                                      aTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACg                                >  1:705841/1‑139 (MQ=255)
                                                                                        catttCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTc                              >  2:92410/4‑139 (MQ=255)
                                                                                             cTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAgg                                                                                                    <  1:797453/64‑1 (MQ=255)
                                                                                             cTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAgg                                                                                                    >  2:797453/1‑64 (MQ=255)
                                                                                                cTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAg                                                             >  2:1164703/1‑100 (MQ=255)
                                                                                                cTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAg                                                             <  1:1164703/100‑1 (MQ=255)
                                                                                                     gatTTCTACCTTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGcaca                                                                                                          <  2:792919/50‑1 (MQ=255)
                                                                                                     gatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGcaca                                                                                                          >  1:792919/1‑50 (MQ=255)
                                                                                                     gatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCa                                  <  1:568955/122‑1 (MQ=255)
                                                                                                     gatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCa                                  >  2:568955/1‑122 (MQ=255)
                                                                                                     gatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCt                 >  2:651671/1‑139 (MQ=255)
                                                                                                        ttCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCtttt              >  2:1127238/1‑139 (MQ=255)
                                                                                                         tCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTtg             <  2:773895/139‑1 (MQ=255)
                                                                                                             ccGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAgg                                                                                                    >  2:314206/1‑48 (MQ=255)
                                                                                                             ccGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAgg                                                                                                    <  1:314206/48‑1 (MQ=255)
                                                                                                             ccGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaa         <  2:140797/139‑1 (MQ=255)
                                                                                                             ccGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaa         <  2:220274/139‑1 (MQ=255)
                                                                                                             ccGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaa         <  1:448625/139‑1 (MQ=255)
                                                                                                               gTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaatt       <  1:1056657/139‑1 (MQ=255)
                                                                                                                tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGa                                                                                                 <  1:170594/48‑1 (MQ=255)
                                                                                                                tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGa                                                                                                 >  2:170594/1‑48 (MQ=255)
                                                                                                                tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTg                                                                          >  2:1439140/1‑71 (MQ=255)
                                                                                                                tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTg                                                                          <  1:1439140/71‑1 (MQ=255)
                                                                                                                tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCac                                                       <  1:58738/90‑1 (MQ=255)
                                                                                                                tCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCac                                                       >  2:58738/1‑90 (MQ=255)
                                                                                                                  tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGtt                                                                                       <  1:849409/56‑1 (MQ=255)
                                                                                                                  tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGtt                                                                                       >  2:849409/1‑56 (MQ=255)
                                                                                                                  tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaattaat    >  1:526822/1‑139 (MQ=255)
                                                                                                                  tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaattaat    <  1:1127238/139‑1 (MQ=255)
                                                                                                                    ggTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCg                                            >  2:1162100/1‑97 (MQ=255)
                                                                                                                    ggTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCg                                            <  1:1162100/97‑1 (MQ=255)
                                                                                                                    ggTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAAttt  <  2:526822/139‑1 (MQ=255)
                                                                                                                         aGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                            >  1:227956/1‑60 (MQ=255)
                                                                                                                         aGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                            <  2:227956/60‑1 (MQ=255)
                                                                                                                                |                                                                                                                              
CCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTT  >  NC_000913/1756878‑1757132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A