Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F1 I1 R1 778 68.7 3228504 93.4% 3015422 109.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,793,792 C→T A353V (GCG→GTG)  gabT → 4‑aminobutyrate aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,793,7920CT100.0% 159.4 / NA 50A353V (GCG→GTG) gabT4‑aminobutyrate aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/28);  total (22/28)

CGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCG  >  NC_000913/2793657‑2793927
                                                                                                                                       |                                                                                                                                       
cgcTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTgat                                                                                                                                      <  2:1152049/139‑1 (MQ=255)
             gAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAg                                                                                                                         <  2:30462/139‑1 (MQ=255)
                    tttGAGCAGGAAAATCTGCTGCAAAAAGCGAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCTGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTg                                                                                                                  >  2:1075753/1‑139 (MQ=255)
                          cAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACg                                                                                                            >  2:180484/1‑139 (MQ=255)
                            ggAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGc                                                                                                          >  1:1592830/1‑139 (MQ=255)
                              aaaaTCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGa                                                                                                        >  2:631496/1‑139 (MQ=255)
                                       tgcAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCAcaacaa                                                                                               >  1:390492/1‑139 (MQ=255)
                                            aaaGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCgg                                                                                          <  2:1356497/139‑1 (MQ=255)
                                            aaaGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCgg                                                                                          <  2:1357351/139‑1 (MQ=255)
                                              aGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGAc                                                                                        <  2:1592830/139‑1 (MQ=255)
                                                   aCGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCgg                                                                                          <  1:379164/132‑1 (MQ=255)
                                                   aCGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCgg                                                                                          >  2:379164/1‑132 (MQ=255)
                                                           gggCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCAccg                                                                           <  1:631496/139‑1 (MQ=255)
                                                               aGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCga                                                                       >  2:163534/1‑139 (MQ=255)
                                                                 aaGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCgaga                                                                     >  2:750610/1‑139 (MQ=255)
                                                                      gAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTg                                                                >  1:551666/1‑139 (MQ=255)
                                                                           aCGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAg                                                                                                                         >  2:1415524/1‑77 (MQ=255)
                                                                           aCGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAg                                                                                                                         <  1:1415524/77‑1 (MQ=255)
                                                                           aCGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTcg                                                           >  1:151228/1‑139 (MQ=255)
                                                                           aCGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTcg                                                           >  2:114892/1‑139 (MQ=255)
                                                                           aCGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTcg                                                           <  2:130444/139‑1 (MQ=255)
                                                                             ggATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACg                                                                                                            >  1:818902/1‑88 (MQ=255)
                                                                             ggATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACg                                                                                                            <  2:818902/88‑1 (MQ=255)
                                                                              gATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACg                                                                                                            <  1:391242/87‑1 (MQ=255)
                                                                              gATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACg                                                                                                            >  2:391242/1‑87 (MQ=255)
                                                                               aTTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGcc                                                       <  1:1305810/139‑1 (MQ=255)
                                                                                 tGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGa                                                                                                                          >  2:1195776/1‑70 (MQ=255)
                                                                                 ggCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGa                                                                                                                          <  1:1195776/69‑1 (MQ=255)
                                                                                      gCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATa                                                <  1:1156439/139‑1 (MQ=255)
                                                                                        gATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATa                                                <  2:982712/137‑1 (MQ=255)
                                                                                        gATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATa                                                >  1:982712/1‑137 (MQ=255)
                                                                                            gCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGcc                                          <  2:151228/139‑1 (MQ=255)
                                                                                             ccGAAAAACACCCGGAGCTCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCt                                         <  1:163534/139‑1 (MQ=255)
                                                                                                     cacCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGcc                                          <  1:694448/130‑1 (MQ=255)
                                                                                                     cacCCGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGcc                                          >  2:694448/1‑130 (MQ=255)
                                                                                                       cccGGAGATCGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTtctc                               >  2:213023/1‑139 (MQ=255)
                                                                                                                cGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGc                      <  1:204457/139‑1 (MQ=255)
                                                                                                                cGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGc                      <  2:577735/139‑1 (MQ=255)
                                                                                                                cGGCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCCCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGc                      <  1:750610/139‑1 (MQ=255)
                                                                                                                 ggCGACGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGcc                     <  1:213023/139‑1 (MQ=255)
                                                                                                                      cGTACGCGGGCTGGGGGTGATGATCGCCATTGCGCCGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTcgcg                                                         >  1:706565/1‑98 (MQ=255)
                                                                                                                      cGTACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTcgcg                                                         <  2:706565/98‑1 (MQ=255)
                                                                                                                      cGTACGCGGGCT‑GGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTAtt               >  2:1008822/1‑139 (MQ=255)
                                                                                                                        tACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATaa                                               <  2:40933/106‑1 (MQ=255)
                                                                                                                        tACGCGGGCTGGGGGTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATaa                                               >  1:40933/1‑106 (MQ=255)
                                                                                                                                  gggggTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCt                                         >  1:1145868/1‑102 (MQ=255)
                                                                                                                                  gggggTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCt                                         <  2:1145868/102‑1 (MQ=255)
                                                                                                                                    gggTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGTCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTgcg  <  2:551666/139‑1 (MQ=255)
                                                                                                                                    gggTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTgcg  <  2:627466/139‑1 (MQ=255)
                                                                                                                                    gggTGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTgcg  <  2:483499/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCG  >  NC_000913/2793657‑2793927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A