Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	778	68.7	3228504	93.4%	3015422	109.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,411	C→T	394 (TAG→TAA)	yqeF ←	short chain acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,411	0	C	T	100.0%	204.0 / NA	64	394 (TAG→TAA)	yqeF	short chain acyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (36/28); total (36/28)

ATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGC  >  NC_000913/2984287‑2984541

aTCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTc                                                                                                                      >  2:1463334/1‑139 (MQ=255)

      aaTATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGt                                                                                                                <  1:1463334/139‑1 (MQ=255)

               tGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                         >  2:181563/1‑137 (MQ=255)

               tGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                         <  1:181563/137‑1 (MQ=255)

                gagaATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCcaca                                                                                                      >  2:295656/1‑139 (MQ=255)

                   aaTGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACAccc                                                                                                   >  1:309664/1‑139 (MQ=255)

                     tGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTg                                                                                                 >  2:240477/1‑139 (MQ=255)

                        aaaaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                              <  1:295656/139‑1 (MQ=255)

                             tGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccgc                                                                                         <  2:1243027/139‑1 (MQ=255)

                                   aGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATAc                                                                                   <  1:635639/139‑1 (MQ=255)

                                      ccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCca                                                                                                        >  1:1404317/1‑115 (MQ=255)

                                      ccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCca                                                                                                        <  2:1404317/115‑1 (MQ=255)

                                                    tCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACaa                                                                                 <  2:286534/124‑1 (MQ=255)

                                                    tCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACaa                                                                                 >  1:286534/1‑124 (MQ=255)

                                                    tCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGc                                                                              <  1:236813/127‑1 (MQ=255)

                                                    tCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGc                                                                              >  2:236813/1‑127 (MQ=255)

                                                       tataagagacagtcatTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGAGTTGCCAGTCCTTTg                                                               <  1:1076585/127‑1 (MQ=255)

                                                          tGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCgg                                                            >  2:1355512/1‑139 (MQ=255)

                                                          tGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCgg                                                            >  1:802833/1‑139 (MQ=255)

                                                              agccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAt                                                        <  2:755490/139‑1 (MQ=255)

                                                               gccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAtt                                                       <  2:802833/139‑1 (MQ=255)

                                                                         ttCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGt                                                                                                                >  2:674932/1‑72 (MQ=255)

                                                                         ttCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGt                                                                                                                <  1:674932/72‑1 (MQ=255)

                                                                         ttCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAg                                                                               >  2:616520/1‑105 (MQ=255)

                                                                         ttCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAg                                                                               <  1:616520/105‑1 (MQ=255)

                                                                              cAGATTAATGACGGCAGATATTTATCGGTCAGGAGGACATGCATAGTTATTCGTCACGTTCAATGGTCAATGCCTCACCCTGGCCCCCGCCGATACAACGCGTTGCCAGTCCTTTGCGGGCATTACgctgtctcttata                                        >  2:1076585/1‑127 (MQ=255)

                                                                              cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                         >  1:78336/1‑74 (MQ=255)

                                                                              cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                         <  2:78336/74‑1 (MQ=255)

                                                                              cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTc                                        >  2:93506/1‑139 (MQ=255)

                                                                               aGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                         >  2:1206689/1‑73 (MQ=255)

                                                                               aGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                         <  1:1206689/73‑1 (MQ=255)

                                                                                 agtAATGACGGCAGATATTTATCGGGAAGGAGGAAATGCAGAGTTATTCGTTACGTTGAATGGTCAATGATACACCCTGGCCGCCGCCGATACAAAGCGTTGCCAGGCCTTTGCGGGCATTACGTTTCACAATTTCATg                                     >  1:408375/3‑139 (MQ=255)

                                                                                 aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg                                     >  2:1383991/1‑139 (MQ=255)

                                                                                 aTTAATGACGGCAGATATCTATCGGTAAGGAGGAAATGCAGAGTTATTCGt                                                                                                                             <  2:529857/51‑1 (MQ=255)

                                                                                 aTTAATGACGGCAGATATCTATCGGTAAGGAGGAAATGCAGAGTTATTCGt                                                                                                                             >  1:529857/1‑51 (MQ=255)

                                                                                   tAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGttt                                                 >  2:1253051/1‑125 (MQ=255)

                                                                                   tAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGttt                                                 <  1:1253051/125‑1 (MQ=255)

                                                                                           gCAGATATTTATCGGTAAGGAGGAAGTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGc                                                              <  1:1060978/104‑1 (MQ=255)

                                                                                           gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGc                                                              >  2:1060978/1‑104 (MQ=255)

                                                                                           gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGaa                           >  1:952094/1‑139 (MQ=255)

                                                                                            cAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                         >  1:908975/1‑60 (MQ=255)

                                                                                            cAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                         <  2:908975/60‑1 (MQ=255)

                                                                                               atatTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCa                       >  2:833845/1‑139 (MQ=255)

                                                                                                         gTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATAc                                                                                   <  1:251055/69‑1 (MQ=255)

                                                                                                         gTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATAc                                                                                   >  2:251055/1‑69 (MQ=255)

                                                                                                         gTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCa             >  1:562289/1‑139 (MQ=255)

                                                                                                         gTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCCTTGCGGGCAt                                                        >  1:1267864/1‑96 (MQ=255)

                                                                                                         gTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCCTTGCGGGCAt                                                        <  2:1267864/96‑1 (MQ=255)

                                                                                                           aaGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAAc           >  1:1130690/1‑139 (MQ=255)

                                                                                                            aggaggAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCagag                             <  2:1372642/120‑1 (MQ=255)

                                                                                                            aggaggAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCagag                             >  1:1372642/1‑120 (MQ=255)

                                                                                                            aggaggAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCaa            >  1:1275520/1‑137 (MQ=255)

                                                                                                            aggaggAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCaa            <  2:1275520/137‑1 (MQ=255)

                                                                                                            aggaggAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAAcc          >  2:106921/1‑139 (MQ=255)

                                                                                                             ggaggaAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCg                                                             <  1:926358/87‑1 (MQ=255)

                                                                                                             ggaggaAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCg                                                             >  2:926358/1‑87 (MQ=255)

                                                                                                                   aaTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgcg   >  2:741950/1‑139 (MQ=255)

                                                                                                                    aTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAtt                                                       <  2:618704/86‑1 (MQ=255)

                                                                                                                    aTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAtt                                                       >  1:618704/1‑86 (MQ=255)

                                                                                                                    aTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAt                                                        >  2:679952/1‑85 (MQ=255)

                                                                                                                    aTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAt                                                        <  1:679952/85‑1 (MQ=255)

                                                                                                                    aTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgcgc  <  2:562289/139‑1 (MQ=255)

                                                                                                                        agagTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTTATGAACCAGAGAAACAAGGATTCGGCAACCGGAAgcg   >  2:282306/1‑134 (MQ=255)

                                                                                                                        agagTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgcg   <  1:282306/134‑1 (MQ=255)

ATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGC  >  NC_000913/2984287‑2984541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A