Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I1 R1 765 39.6 1867486 94.4% 1762906 108.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 649,648 A→G G281G (GGT→GGC)  citE ← citrate lyase, citryl‑ACP lyase (beta) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913649,6480AG100.0% 68.2 / NA 20G281G (GGT→GGC) citEcitrate lyase, citryl‑ACP lyase (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/10);  total (10/10)

ACGATTCCAGGCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGG  >  NC_000913/649515‑649765
                                                                                                                                     |                                                                                                                     
acgaTTCCAGGCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTc                                                                                                                  <  2:395051/139‑1 (MQ=255)
              cTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCg                                                                                                     >  1:688380/1‑138 (MQ=255)
              cTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCg                                                                                                     <  2:688380/138‑1 (MQ=255)
                                       ttCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAggg                                                                                              >  2:772353/1‑120 (MQ=255)
                                       ttCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAggg                                                                                              <  1:772353/120‑1 (MQ=255)
                                            ttttCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAg                                                                      >  1:324482/1‑139 (MQ=255)
                                                 tGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAgcggcg                                                                 >  2:829455/1‑139 (MQ=255)
                                                       aTTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCa                                                           <  2:113075/139‑1 (MQ=255)
                                                                       tcttcGCGGATGCCGGAAAGTTCTGCACGGGAGAGCAGCAGACTGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCTAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGAcgc                                           >  1:622974/1‑139 (MQ=255)
                                                                           cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTccacca                                                                                                             <  2:97237/69‑1 (MQ=255)
                                                                           cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTccacca                                                                                                             >  1:97237/1‑69 (MQ=255)
                                                                           cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCg                                                                                   <  2:582027/95‑1 (MQ=255)
                                                                           cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcggcg                                       >  1:824788/1‑139 (MQ=255)
                                                                           cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcggcg                                       >  1:544930/1‑139 (MQ=255)
                                                                           cgcgGATGCCGGAAAGTACTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCg                                                                                   >  1:582027/1‑95 (MQ=255)
                                                                                   ccGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGAt                               <  2:544930/139‑1 (MQ=255)
                                                                                    cGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATc                              <  1:829455/139‑1 (MQ=255)
                                                                                                      agagCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCgg            <  2:824788/139‑1 (MQ=255)
                                                                                                               gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTagag   >  2:873763/1‑139 (MQ=255)
                                                                                                                aCGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAgg  <  1:649739/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                     
ACGATTCCAGGCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGG  >  NC_000913/649515‑649765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A