Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I1 R1 765 39.6 1867486 94.4% 1762906 108.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 664,102 C→T *363* (TAG→TAA)  rlpA ← septal ring protein, suppressor of prc, minor lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913664,1020CT100.0% 200.5 / NA 62*363* (TAG→TAA) rlpAseptal ring protein, suppressor of prc, minor lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/31);  total (31/31)

AACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCG  >  NC_000913/663970‑664233
                                                                                                                                    |                                                                                                                                   
aaCTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                                               >  2:125134/1‑139 (MQ=255)
 aCTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              <  1:125134/139‑1 (MQ=255)
 aCTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              <  1:759395/139‑1 (MQ=255)
   tACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGcgcg                                                                                                                            <  2:314412/139‑1 (MQ=255)
   tACGACATCCGTGATGGAATTAAAAAACTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCACTTACAGAAATTGACAGATCAGATGCCTGCTTTACGTTACTGcgcg                                                                                                                            <  2:536196/139‑1 (MQ=255)
     cGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                                          >  2:170195/1‑139 (MQ=255)
        cATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGcgcg                                                                                                                            >  1:229258/1‑134 (MQ=255)
        cATCCGTGATGGAATTAAAAAAGTGCCCTAATATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGcgcg                                                                                                                            <  2:229258/134‑1 (MQ=255)
          tCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGtaa                                                                                                                     >  1:91009/1‑139 (MQ=255)
               gATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAAt                                                                                                                <  2:91009/139‑1 (MQ=255)
                   gAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              >  2:450733/1‑121 (MQ=255)
                   gAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              <  1:450733/121‑1 (MQ=255)
                        aaaaaaGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                                          >  1:299196/1‑120 (MQ=255)
                        aaaaaaGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                                          <  2:299196/120‑1 (MQ=255)
                                 cccTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTCCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgta                                                                                                                         <  2:271067/112‑1 (MQ=255)
                                 cccTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTCCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgta                                                                                                                         >  1:271067/1‑112 (MQ=255)
                                        ataGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCa                                                                                       <  1:728974/139‑1 (MQ=255)
                                         taGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCaa                                                                                      <  2:690543/139‑1 (MQ=255)
                                            cAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                                               <  2:896500/95‑1 (MQ=255)
                                            cAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                                               >  1:896500/1‑95 (MQ=255)
                                             aaaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTAACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGt                                                                                  <  1:134014/139‑1 (MQ=255)
                                              aaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              >  2:289052/1‑94 (MQ=255)
                                              aaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              <  1:289052/94‑1 (MQ=255)
                                              aaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                                               <  2:24044/93‑1 (MQ=255)
                                              aaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                                               >  1:24044/1‑93 (MQ=255)
                                                tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              >  1:761263/1‑92 (MQ=255)
                                                tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              <  2:239784/92‑1 (MQ=255)
                                                tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              <  2:761263/92‑1 (MQ=255)
                                                tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                                          >  2:684983/1‑96 (MQ=255)
                                                tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                                          <  1:684983/96‑1 (MQ=255)
                                                tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGc                                                                                                  <  2:131611/120‑1 (MQ=255)
                                                tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGc                                                                                                  >  1:131611/1‑120 (MQ=255)
                                                tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgc                                                                               >  2:677142/1‑139 (MQ=255)
                                                tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgc                                                                               >  2:900786/1‑139 (MQ=255)
                                                tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgc                                                                               >  1:830000/1‑139 (MQ=255)
                                                tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgc                                                                               >  1:68429/1‑139 (MQ=255)
                                                tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTACTTTACGTTACTgcg                                                                                                                              >  1:239784/1‑92 (MQ=255)
                                                    cTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                                           <  2:68429/139‑1 (MQ=255)
                                                    cTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                                           <  1:677142/139‑1 (MQ=255)
                                                          gcaggcaTCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTAc                                                                     >  1:383268/1‑139 (MQ=255)
                                                                 tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              >  2:638155/1‑75 (MQ=255)
                                                                 tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              <  1:638155/75‑1 (MQ=255)
                                                                 tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTc                                                              >  1:313092/1‑139 (MQ=255)
                                                                     aCTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCt                                                          <  1:900786/139‑1 (MQ=255)
                                                                             cgTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              >  1:619186/1‑63 (MQ=255)
                                                                             cgTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                                              <  2:619186/63‑1 (MQ=255)
                                                                               tGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGa                                                >  2:736415/1‑139 (MQ=255)
                                                                                gACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGaa                                               <  2:313092/139‑1 (MQ=255)
                                                                                            gTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTa                                                                                                                                   >  2:402297/1‑43 (MQ=255)
                                                                                            gTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTg                                   >  2:43364/1‑139 (MQ=255)
                                                                                             tCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTgg                                  <  1:43364/139‑1 (MQ=255)
                                                                                               aTTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGAt                                >  2:262799/1‑139 (MQ=255)
                                                                                               aTTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGAt                                >  2:755809/1‑139 (MQ=255)
                                                                                                 ttACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGc                                          <  2:221782/127‑1 (MQ=255)
                                                                                                 ttACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGc                                          >  1:221782/1‑127 (MQ=255)
                                                                                                          ttGACACATCAGATGCCTGCTTTACGTTACTGcgcg                                                                                                                            <  2:606194/36‑1 (MQ=255)
                                                                                                          ttGACACATCAGATGCCTGCTTTACGTTACTGcgcg                                                                                                                            >  1:606194/1‑36 (MQ=255)
                                                                                                                   cAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCAtttt            >  2:86979/1‑139 (MQ=255)
                                                                                                                         ccTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTa      <  2:830000/139‑1 (MQ=255)
                                                                                                                             cTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGcc                                         <  1:505979/100‑1 (MQ=255)
                                                                                                                             cTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGcc                                         >  2:505979/1‑100 (MQ=255)
                                                                                                                             cTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTAcgcg  <  1:262799/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
AACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCGCCATTTTGAGTTACGCG  >  NC_000913/663970‑664233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A