Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,212,088 |
(C)8→9 |
intergenic (‑85/+615) |
iraM ← / ← ycgX |
RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,212,080 | 1 | . | C | 92.3%
| 34.4
/ ‑2.6
| 13 | intergenic (‑77/+623) | iraM/ycgX | RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (7/5); total (8/5) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01 |
GTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCA > NC_000913/1211981‑1212187
|
gtgtCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGc < 2:716655/139‑1 (MQ=255)
ttCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAAttt > 1:705728/1‑139 (MQ=255)
cATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaa < 2:753908/139‑1 (MQ=255)
aTTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaaa > 2:623538/1‑139 (MQ=255)
gTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGaaa > 1:71385/1‑139 (MQ=255)
tCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAAt < 2:705728/139‑1 (MQ=255)
cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAACCTCAAt < 1:77396/134‑1 (MQ=255)
cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAACCTCAAt > 2:77396/1‑134 (MQ=255)
ggTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCATCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGt > 1:61712/1‑139 (MQ=255)
ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATgaaa > 1:456097/1‑137 (MQ=255)
ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAAAAAAATgaga > 2:375037/1‑139 (MQ=255)
tGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGaaa > 2:553325/1‑139 (MQ=255)
ttATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa < 1:553325/139‑1 (MQ=255)
|
GTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCC‑TCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCA > NC_000913/1211981‑1212187
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
