Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,228,724 |
A→G |
T216A (ACT→GCT) |
ycgM → |
putative isomerase/hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,228,724 | 0 | A | G | 100.0%
| 49.2
/ NA
| 15 | T216A (ACT→GCT) | ycgM | putative isomerase/hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (8/7); total (8/7) |
AGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAG > NC_000913/1228607‑1228834
|
aGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACtttt > 2:186296/1‑139 (MQ=255)
aCGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGGTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTg > 2:916671/1‑139 (MQ=255)
gtgtTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCg < 1:186296/139‑1 (MQ=255)
cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCaa < 1:137000/117‑1 (MQ=255)
cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCaa > 2:137000/1‑117 (MQ=255)
cTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc > 1:95545/1‑134 (MQ=255)
cTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc < 2:95545/134‑1 (MQ=255)
tCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGc > 1:677852/1‑139 (MQ=255)
ttGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAAc > 1:773599/1‑139 (MQ=255)
gTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCgg > 2:94752/1‑139 (MQ=255)
gatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGaa < 1:94752/139‑1 (MQ=255)
gatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGaa < 2:677852/139‑1 (MQ=255)
aGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAg < 2:478665/139‑1 (MQ=255)
tttCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGaa < 1:489076/122‑1 (MQ=255)
tttCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGaa > 2:489076/1‑122 (MQ=255)
|
AGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAG > NC_000913/1228607‑1228834
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
