Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,424,386	G→A	229 (TAG→TAA)	ulaF →	L‑ribulose 5‑phosphate 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,424,386	0	G	A	100.0%	73.5 / NA	24	229 (TAG→TAA)	ulaF	L‑ribulose 5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/14); total (10/14)

AAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTAT  >  NC_000913/4424262‑4424507

aagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCgg                                                                                                             <  1:848676/139‑1 (MQ=255)

           aTGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATttccttc                                                                                                  <  2:152409/139‑1 (MQ=255)

                                                                          aaaCACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggggg                                   <  1:100086/139‑1 (MQ=255)

                                                                          aaaCACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggggg                                   <  1:314085/139‑1 (MQ=255)

                                                                                 tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACAcg                                                                                                                     >  2:667725/1‑50 (MQ=255)

                                                                                 tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACAcg                                                                                                                     <  1:667725/50‑1 (MQ=255)

                                                                                 tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct                                                                                                <  1:664012/71‑1 (MQ=255)

                                                                                 tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct                                                                                                >  2:664012/1‑71 (MQ=255)

                                                                                 tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagca                            <  1:465330/139‑1 (MQ=255)

                                                                                 tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagca                            <  2:594159/139‑1 (MQ=255)

                                                                                  cATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagcaa                           >  2:282148/1‑139 (MQ=255)

                                                                                        tAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAAttt                                                                                                       >  2:748978/1‑57 (MQ=255)

                                                                                        tAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAAttt                                                                                                       <  1:748978/57‑1 (MQ=255)

                                                                                          aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGc                                                  <  1:434865/108‑1 (MQ=255)

                                                                                          aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGc                                                  >  2:434865/1‑108 (MQ=255)

                                                                                               ggTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTg                                                                                                                <  1:603916/41‑1 (MQ=255)

                                                                                               ggTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTg                                                                                                                >  2:603916/1‑41 (MQ=255)

                                                                                               ggTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct                                                                                                >  1:25261/1‑57 (MQ=255)

                                                                                               ggTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct                                                                                                <  2:25261/57‑1 (MQ=255)

                                                                                                gTCCTAACGCTTATTACGGGCAGAAGTAAAACACgcgc                                                                                                                  <  1:68149/38‑1 (MQ=255)

                                                                                                gTCCTAACGCTTATTACGGGCAGAAGTAAAACACgcgc                                                                                                                  >  2:68149/1‑38 (MQ=255)

                                                                                                           tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGt     <  2:467266/136‑1 (MQ=255)

                                                                                                           tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGt     >  1:467266/1‑136 (MQ=255)

                                                                                                           tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtat  >  1:882438/1‑139 (MQ=255)

AAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTAT  >  NC_000913/4424262‑4424507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A