Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,425,834	G→A	105 (TAG→TAA)	priB →	primosomal protein N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,425,834	0	G	A	100.0%	116.8 / NA	37	105 (TAG→TAA)	priB	primosomal protein N
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/18); total (19/18)

GCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCA  >  NC_000913/4425700‑4425961

gCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCat                                                                                                                             >  2:670974/1‑139 (MQ=255)

 ccATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCata                                                                                                                            <  1:670974/139‑1 (MQ=255)

        tCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACg                                                                                                                     <  1:265013/139‑1 (MQ=255)

                 aaCGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCcg                                                                                                            >  2:780694/1‑139 (MQ=255)

                   cGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCcgtc                                                                                                          >  1:502480/1‑139 (MQ=255)

                      tCGGCAGTCGCATAATCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGCGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCgtcgtc                                                                                                       >  2:146376/1‑139 (MQ=255)

                      tCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCAC‑AGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGtcgtcg                                                                                                      >  2:37916/1‑139 (MQ=255)

                        ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATgg                                                                                                                         >  1:140152/1‑119 (MQ=255)

                        ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATgg                                                                                                                         <  2:140152/119‑1 (MQ=255)

                                        ttCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCa                                                                                     >  2:778060/1‑139 (MQ=255)

                                        ttCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCa                                                                                     >  1:341169/1‑139 (MQ=255)

                                          cAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATgg                                                                                                                         <  1:835677/101‑1 (MQ=255)

                                          cAGGGGTTCATTTCATGCCACAAGGCAAAGAACCGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATgg                                                                                                                         >  2:835677/1‑101 (MQ=255)

                                                ttcatttcatGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGaa                                                                             >  1:111740/1‑139 (MQ=255)

                                                 tcatttcatGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                            <  1:388874/139‑1 (MQ=255)

                                                  catttcatGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAgg                                                                           >  2:298807/1‑139 (MQ=255)

                                                             acaAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAgaga                                                                >  2:445267/1‑139 (MQ=255)

                                                                      aGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAt                                                               <  2:798453/131‑1 (MQ=255)

                                                                      aGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAt                                                               >  1:798453/1‑131 (MQ=255)

                                                                          cGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGa                                                   <  1:780694/139‑1 (MQ=255)

                                                                               tGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCg                                              <  1:37916/139‑1 (MQ=255)

                                                                               tGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCg                                              <  1:298807/139‑1 (MQ=255)

                                                                                gAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGc                                             >  2:861786/1‑139 (MQ=255)

                                                                                 aGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAt                                                               <  1:410425/120‑1 (MQ=255)

                                                                                 aGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAt                                                               >  2:410425/1‑120 (MQ=255)

                                                                                           ttttGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAt                                                               >  1:901845/1‑110 (MQ=255)

                                                                                           tgttGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAt                                                               <  2:901845/108‑1 (MQ=255)

                                                                                                         cAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaa                    <  2:111740/139‑1 (MQ=255)

                                                                                                         cAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaa                    <  1:823368/139‑1 (MQ=255)

                                                                                                            attgaattgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAt                                                               <  2:895036/93‑1 (MQ=255)

                                                                                                            attgaattgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAt                                                               >  1:895036/1‑93 (MQ=255)

                                                                                                               gaattgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGt              <  2:502480/139‑1 (MQ=255)

                                                                                                                   tgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGa          <  2:341169/139‑1 (MQ=255)

                                                                                                                      tAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGa                                                            >  1:287385/1‑86 (MQ=255)

                                                                                                                      tAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGa                                                            <  2:287385/86‑1 (MQ=255)

                                                                                                                          ttCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGa          <  1:93929/132‑1 (MQ=255)

                                                                                                                          ttCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGa          >  2:93929/1‑132 (MQ=255)

                                                                                                                           tcttgcgaCTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACTGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCa  <  2:362124/133‑1 (MQ=255)

GCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCA  >  NC_000913/4425700‑4425961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A