Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F1 I1 R1 759 65.2 3386260 90.7% 3071337 101.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,353,121 C→T *310* (TAG→TAA)  yhcC ← putative Fe‑S oxidoreductase, Radical SAM superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,353,1210CT100.0% 212.4 / NA 65*310* (TAG→TAA) yhcCputative Fe‑S oxidoreductase, Radical SAM superfamily protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/30);  total (35/30)

GTCAACATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGG  >  NC_000913/3352987‑3353250
                                                                                                                                        |                                                                                                                                 
gTCAACATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACt                                                                                                                               <  1:1429028/139‑1 (MQ=255)
    aCATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGt                                                                                                                           >  2:28254/1‑139 (MQ=255)
               cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGcc                                                                                                                >  1:1061770/1‑139 (MQ=255)
                                      ttAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATc                                                                                           >  2:725243/1‑139 (MQ=255)
                                      ttAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATccc                                                                                         >  2:934349/1‑139 (MQ=255)
                                         gggAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGt                                                                                      >  2:1282211/1‑139 (MQ=255)
                                              ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                               <  2:898007/125‑1 (MQ=255)
                                              ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                               >  1:898007/1‑125 (MQ=255)
                                              ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGcc                                                                                 >  2:711443/1‑139 (MQ=255)
                                              ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGcc                                                                                 >  1:387649/1‑139 (MQ=255)
                                               tCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCa                                                                                >  2:925669/1‑139 (MQ=255)
                                                 cTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATg                                                                              <  2:618440/139‑1 (MQ=255)
                                                    cACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttc                                                                           <  2:387649/139‑1 (MQ=255)
                                                     aCGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttca                                                                          >  1:1481281/1‑139 (MQ=255)
                                                         cAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcattca                                                                      <  1:934349/139‑1 (MQ=255)
                                                            ccTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGAt                                                                   >  2:1112082/1‑139 (MQ=255)
                                                                ccccAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAgg                                                                                                                     <  1:1550642/85‑1 (MQ=255)
                                                                ccccAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAgg                                                                                                                     >  2:1550642/1‑85 (MQ=255)
                                                                ccccAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCg                                                               >  2:1684232/1‑139 (MQ=255)
                                                                 cccAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGa                                                              >  2:260559/1‑139 (MQ=255)
                                                                     ataataCCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGt                                                                                      >  2:105775/1‑111 (MQ=255)
                                                                     ataataCCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGt                                                                                      <  1:105775/111‑1 (MQ=255)
                                                                         taCCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTc                                                      <  2:1481281/139‑1 (MQ=255)
                                                                           cccAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGa                                                    >  1:961541/1‑139 (MQ=255)
                                                                           cccAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGACCCAGCGCCGATCCCTGTACGCCATGttcat                                                                         <  1:761374/118‑1 (MQ=255)
                                                                           cccAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGACCCAGCGCCGATCCCTGTACGCCATGttcat                                                                         >  2:761374/1‑118 (MQ=255)
                                                                                ttCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAg                                                                                                                      >  1:1678503/1‑68 (MQ=255)
                                                                                ttCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAg                                                                                                                      <  2:1678503/68‑1 (MQ=255)
                                                                                ttCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcattca                                                                      >  1:287614/1‑116 (MQ=255)
                                                                                ttCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcattca                                                                      <  2:287614/116‑1 (MQ=255)
                                                                                     aCAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcattca                                                                      >  1:435862/1‑111 (MQ=255)
                                                                                     aCAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcattca                                                                      <  2:435862/111‑1 (MQ=255)
                                                                                      cAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCa                                                                                >  2:334745/1‑100 (MQ=255)
                                                                                      cAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCa                                                                                <  1:334745/100‑1 (MQ=255)
                                                                                        gTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCa                                       <  1:711443/139‑1 (MQ=255)
                                                                                         ttACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATg                                                                              <  1:1599375/99‑1 (MQ=255)
                                                                                         ttACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATg                                                                              >  2:1599375/1‑99 (MQ=255)
                                                                                          tACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAg                                                         <  2:1388050/119‑1 (MQ=255)
                                                                                          tACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAg                                                         >  1:1388050/1‑119 (MQ=255)
                                                                                           aCGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATc                                                                                           >  2:259967/1‑84 (MQ=255)
                                                                                           aCGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATc                                                                                           <  1:259967/84‑1 (MQ=255)
                                                                                             gTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGAt                                  <  1:1112082/139‑1 (MQ=255)
                                                                                                     t‑‑tGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcac                          <  2:1061770/139‑1 (MQ=255)
                                                                                                         gtgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcacca                        >  1:561275/1‑139 (MQ=255)
                                                                                                         gtgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGca                           <  1:407961/136‑1 (MQ=255)
                                                                                                         gtgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGca                           >  2:407961/1‑136 (MQ=255)
                                                                                                          tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAgg                                                                                                                     >  2:1338257/1‑45 (MQ=255)
                                                                                                          tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAgg                                                                                                                     <  1:1338257/45‑1 (MQ=255)
                                                                                                          tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAgcg                                                                                                 >  2:860398/1‑65 (MQ=255)
                                                                                                          tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAgcg                                                                                                 <  1:860398/65‑1 (MQ=255)
                                                                                                           gtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                            >  2:1489344/1‑69 (MQ=255)
                                                                                                           gtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                            <  1:1489344/69‑1 (MQ=255)
                                                                                                           gtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACcaca                      <  2:961541/139‑1 (MQ=255)
                                                                                                            tgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAg                     >  1:1288893/1‑139 (MQ=255)
                                                                                                             gagaAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGc                    <  1:725243/139‑1 (MQ=255)
                                                                                                                    gCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGAt                                  <  2:1143273/118‑1 (MQ=255)
                                                                                                                    gCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGAt                                  >  1:1143273/1‑118 (MQ=255)
                                                                                                                     cGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGagcaag            >  1:766370/1‑139 (MQ=255)
                                                                                                                      ggAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGa                                                    <  1:1528048/98‑1 (MQ=255)
                                                                                                                      ggAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGa                                                    >  2:1528048/1‑98 (MQ=255)
                                                                                                                       gAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGagcaagca          <  2:561275/139‑1 (MQ=255)
                                                                                                                       gAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGagcaagca          <  1:1282211/139‑1 (MQ=255)
                                                                                                                          ttCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGa                                                    <  1:269615/94‑1 (MQ=255)
                                                                                                                          ttCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGa                                                    >  2:269615/1‑94 (MQ=255)
                                                                                                                               cTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCggg  >  2:462112/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                 
GTCAACATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAAT‑‑TGTGTGAGAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGG  >  NC_000913/3352987‑3353250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A