Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A10 F1 I1 R1 | 759 | 65.2 | 3386260 | 90.7% | 3071337 | 101.6 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,279,849 | A→G | intergenic (+21/‑131) | ghxP → / → yjcE | guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,279,849 | 0 | A | G | 100.0% | 224.7 / NA | 62 | intergenic (+21/‑131) | ghxP/yjcE | guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (32/30); total (32/30) | |||||||||||
GCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCA > NC_000913/4279715‑4279980 | gcgACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg < 1:569840/138‑1 (MQ=255)gcgACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg > 2:569840/1‑138 (MQ=255) tGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGcc < 2:145043/139‑1 (MQ=255) gtcgtcTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCg < 1:1357756/139‑1 (MQ=255) tttCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAttt > 2:402238/1‑139 (MQ=255) tCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTtc > 2:1038867/1‑139 (MQ=255) tCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTtc > 2:470516/1‑139 (MQ=255) cGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTtctc > 1:685471/1‑139 (MQ=255) aaTGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAt > 2:1618242/1‑128 (MQ=255) aaTGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAt < 1:1618242/128‑1 (MQ=255) aTGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca < 1:1038867/139‑1 (MQ=255) aTGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca < 2:685471/139‑1 (MQ=255) cgcAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGtt < 2:1443571/118‑1 (MQ=255) cgcAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGtt > 1:1443571/1‑118 (MQ=255) cAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCg > 1:906180/1‑114 (MQ=255) cAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCg < 2:906180/114‑1 (MQ=255) gTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAg < 1:470516/139‑1 (MQ=255) aaCATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCttt > 1:735039/1‑139 (MQ=255) ggTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGat > 2:1603320/1‑139 (MQ=255) tCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTg > 2:484145/1‑138 (MQ=255) tCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTg < 1:484145/138‑1 (MQ=255) gTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGaaa > 1:1466423/1‑139 (MQ=255) tATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAt < 2:285299/97‑1 (MQ=255) tATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAt > 1:285299/1‑97 (MQ=255) gACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa < 2:1466423/139‑1 (MQ=255) aTGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca < 1:1416619/83‑1 (MQ=255) aTGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca > 2:1416619/1‑83 (MQ=255) gggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCa < 1:402238/139‑1 (MQ=255) gggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCa < 2:735039/139‑1 (MQ=255) gTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGa < 1:1603320/139‑1 (MQ=255) gTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGa > 2:1319670/1‑139 (MQ=255) ggCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca < 1:1271882/75‑1 (MQ=255) ggCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca > 2:1271882/1‑75 (MQ=255) gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGc > 2:1356875/1‑43 (MQ=255) gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGc < 1:1356875/43‑1 (MQ=255) gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAAt < 2:360978/107‑1 (MQ=255) gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAAt > 1:360978/1‑107 (MQ=255) gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATgag < 1:7863/110‑1 (MQ=255) gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATgag > 2:7863/1‑110 (MQ=255) ggCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaa > 2:669762/1‑139 (MQ=255) cTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCg > 2:1457694/1‑85 (MQ=255) cTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCg < 1:1457694/85‑1 (MQ=255) tATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaaaaa > 2:962228/1‑139 (MQ=255) cTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca < 1:968552/64‑1 (MQ=255) cTAATCTTTCATCGGATACTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca > 2:968552/1‑64 (MQ=255) tCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCa > 2:184343/1‑139 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAt > 1:1441509/1‑43 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTAt < 2:1441509/43‑1 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATAtt < 2:1377064/77‑1 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATAtt > 1:1377064/1‑77 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAAtt < 1:849972/95‑1 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAAtt > 2:849972/1‑95 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAAtt > 2:1366009/1‑95 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAAtt < 1:1366009/95‑1 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa < 1:307709/103‑1 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa > 2:307709/1‑103 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAg > 2:600114/1‑139 (MQ=255) cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAg > 1:566229/1‑139 (MQ=255) aTCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAgg < 1:184343/139‑1 (MQ=255) ttCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca < 2:628247/48‑1 (MQ=255) ttCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca > 1:628247/1‑48 (MQ=255) tCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCa < 1:669762/139‑1 (MQ=255) | GCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCA > NC_000913/4279715‑4279980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |