Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,325,152	A→G	I5T (ATC→ACC)	phnC ←	phosphonate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,325,152	0	A	G	100.0%	203.8 / NA	57	I5T (ATC→ACC)	phnC	phosphonate ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/28); total (29/28)

ACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCGATGACAGTGCGGGAGGGCGGGC  >  NC_000913/4325022‑4325286

aCGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGtt                                                                                                                                 >  1:112295/1‑139 (MQ=255)

 cGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGttt                                                                                                                                >  1:121976/1‑139 (MQ=255)

 cGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGttt                                                                                                                                >  1:429786/1‑139 (MQ=255)

  gTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTg                                                                                                                               <  1:1610548/139‑1 (MQ=255)

           tGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGt                                                                                                                                  >  2:1351050/1‑127 (MQ=255)

           tGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGt                                                                                                                                  <  1:1351050/127‑1 (MQ=255)

             gATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTg                                                                                                                               >  2:744981/1‑128 (MQ=255)

             gATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTg                                                                                                                               <  1:744981/128‑1 (MQ=255)

             gATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                                    <  1:1028313/139‑1 (MQ=255)

             gATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                                    <  2:112295/139‑1 (MQ=255)

             gATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                                    >  2:1028313/1‑139 (MQ=255)

                   ccGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCt                                                                                                              >  1:6331/1‑139 (MQ=255)

                          ccGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAg                                                                                                      <  2:6331/139‑1 (MQ=255)

                            gACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTcc                                                                                                                >  2:914928/1‑128 (MQ=255)

                            gACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTcc                                                                                                                <  1:914928/128‑1 (MQ=255)

                            gACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTg                                                                                                    >  2:1689890/1‑139 (MQ=255)

                                           gCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCgg                                                                                     >  1:164300/1‑139 (MQ=255)

                                                               aaTGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                                    <  2:580587/89‑1 (MQ=255)

                                                               aaTGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                                    >  1:580587/1‑89 (MQ=255)

                                                                   ttCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAATGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGc                                                                                                                              <  1:760677/75‑1 (MQ=255)

                                                                   ttCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGc                                                                                                                              >  2:760677/1‑75 (MQ=255)

                                                                    tCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGttaatta                                                            >  1:555865/1‑139 (MQ=255)

                                                                      aGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGa                                                                        >  2:781020/1‑125 (MQ=255)

                                                                      aGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGa                                                                        <  1:781020/125‑1 (MQ=255)

                                                                       gATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTaaaa                                                         >  1:24811/1‑139 (MQ=255)

                                                                        aTCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                                    >  2:1605225/1‑80 (MQ=255)

                                                                        aTCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                                    <  1:1605225/80‑1 (MQ=255)

                                                                           aaCCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTgg                                                                   <  1:1647498/125‑1 (MQ=255)

                                                                           aaCCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTgg                                                                   >  2:1647498/1‑125 (MQ=255)

                                                                             ccGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACt                                                   <  2:555865/139‑1 (MQ=255)

                                                                             ccGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACt                                                   <  2:24811/139‑1 (MQ=255)

                                                                               gCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTaa                                                 <  1:1689890/139‑1 (MQ=255)

                                                                                  tGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                                    <  1:1395611/70‑1 (MQ=255)

                                                                                  tGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                                    >  2:1395611/1‑70 (MQ=255)

                                                                                  tGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTg                                                                                         <  1:1304689/96‑1 (MQ=255)

                                                                                  tGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTg                                                                                         >  2:1304689/1‑96 (MQ=255)

                                                                                  tGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTaa                                                 <  2:445986/136‑1 (MQ=255)

                                                                                  tGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTaa                                                 >  1:445986/1‑136 (MQ=255)

                                                                                       cgcCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGttaa                                                               >  1:1201634/1‑117 (MQ=255)

                                                                                       cgcCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGttaa                                                               <  2:1201634/117‑1 (MQ=255)

                                                                                       cgcCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGaa                                         <  1:556448/139‑1 (MQ=255)

                                                                                         ccTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGAGACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGttaat                                                              <  2:1018988/116‑1 (MQ=255)

                                                                                         ccTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAATGGCTCCATCGTGGCGGATTATTGTGACGATTTGGttaat                                                              >  1:1018988/1‑116 (MQ=255)

                                                                                            gATGCTGATTGAAGGTTTTGGCGAGCTTGTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACt                                                   >  2:786423/1‑124 (MQ=255)

                                                                                            gATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACt                                                   <  1:786423/124‑1 (MQ=255)

                                                                                                  gATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACggg                              <  1:1532878/139‑1 (MQ=255)

                                                                                                    ttGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGaa                            >  2:1376020/1‑139 (MQ=255)

                                                                                                            tttGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCGATGa                    <  2:164300/139‑1 (MQ=255)

                                                                                                             ttGGCGAGCTTCTCGACACGGGTAATCGTTTGCat                                                                                                                            >  2:1246659/1‑35 (MQ=255)

                                                                                                             ttGGCGAGCTTCTCGACACGGGTAATCGTTTGCat                                                                                                                            <  1:1246659/35‑1 (MQ=255)

                                                                                                             ttGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTgg                                                                   >  2:133732/1‑92 (MQ=255)

                                                                                                             ttGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTgg                                                                   <  1:133732/92‑1 (MQ=255)

                                                                                                             ttGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCGATGAc                   >  1:576137/1‑139 (MQ=255)

                                                                                                              tGGCGAGCTTCTCGACACGGGTAATCGTTTGTATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCg                                                                                      <  2:1296158/71‑1 (MQ=255)

                                                                                                              tGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCg                                                                                      >  1:1296158/1‑71 (MQ=255)

                                                                                                                     cTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCGATGACAGTGCggg           >  1:348179/1‑139 (MQ=255)

                                                                                                                              acGGGTAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCGATGACAGTGCGGGAgggcgggc  <  1:1376020/139‑1 (MQ=255)

ACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCTCCCT‑AAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCGATGACAGTGCGGGAGGGCGGGC  >  NC_000913/4325022‑4325286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A