Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F1 I1 R1 759 65.2 3386260 90.7% 3071337 101.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,500,043 C→T pseudogene (492/942 nt) yjgX ← pseudogene fragment

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,500,0430CT100.0% 196.8 / NA 61pseudogene (492/942 nt)yjgXpseudogene fragment
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/33);  total (28/33)

TGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGCTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTA  >  NC_000913/4499919‑4500169
                                                                                                                            |                                                                                                                              
tGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCt                                                                                                                  >  1:1293948/1‑139 (MQ=255)
      aaTGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTtaca                                                                                                            <  2:940882/139‑1 (MQ=255)
          aCGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGttac                                                                                                             >  1:1630454/1‑131 (MQ=255)
          aCGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTtac                                                                                                             <  2:1630454/134‑1 (MQ=255)
           cGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGAt                                                                                                       >  1:277096/1‑139 (MQ=255)
            gTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGAtt                                                                                                      >  2:840613/1‑139 (MQ=255)
            gTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGAtt                                                                                                      <  1:286450/139‑1 (MQ=255)
                 tACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGAt                                                                                                 <  2:672842/139‑1 (MQ=255)
                    cGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATgag                                                                                              <  2:277096/139‑1 (MQ=255)
                    cGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATgag                                                                                              <  2:1422092/139‑1 (MQ=255)
                    cGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATgag                                                                                              <  2:1293948/139‑1 (MQ=255)
                              tACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCcatcat                                                                                    >  2:1390775/1‑139 (MQ=255)
                                ccATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTacac                                                                                                           >  1:1265833/1‑114 (MQ=255)
                                ccATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTacac                                                                                                           <  2:1265833/114‑1 (MQ=255)
                                   tCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCa                                                                               <  1:840613/139‑1 (MQ=255)
                                    cAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAg                                                                              <  1:704578/139‑1 (MQ=255)
                                      aGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGa                                                                            >  1:202806/1‑139 (MQ=255)
                                            ccAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTtac                                                                                                             >  1:501131/1‑100 (MQ=255)
                                            ccAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTtac                                                                                                             <  2:501131/100‑1 (MQ=255)
                                                gAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCtt                                                                  <  2:202806/139‑1 (MQ=255)
                                                     ttATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGttac                                                                                                             <  2:252045/91‑4 (MQ=255)
                                                     ttATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGttac                                                                                                             >  1:252045/1‑88 (MQ=255)
                                                        tCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAgg                                                          <  1:1125967/139‑1 (MQ=255)
                                                              ttACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCtt                                                                  >  1:1125616/1‑125 (MQ=255)
                                                              ttACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCtt                                                                  <  2:1125616/125‑1 (MQ=255)
                                                               tACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCcatc                                                                                      >  1:610516/1‑104 (MQ=255)
                                                               tACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCcatc                                                                                      <  2:610516/104‑1 (MQ=255)
                                                                       cTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGtt                                                                                                                        >  1:1028979/1‑62 (MQ=255)
                                                                       cTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGtt                                                                                                                        <  2:1028979/62‑1 (MQ=255)
                                                                       cTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAgcg                                           >  1:66843/1‑139 (MQ=255)
                                                                         ggCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCa                                                                               >  1:680108/1‑101 (MQ=255)
                                                                         ggCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCa                                                                               <  2:680108/101‑1 (MQ=255)
                                                                          gCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCAc                                        >  1:1671165/1‑139 (MQ=255)
                                                                             aGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGtt                                     <  2:1671165/139‑1 (MQ=255)
                                                                             aGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGtt                                     <  2:706504/139‑1 (MQ=255)
                                                                               gTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAgg                                                          >  2:577775/1‑116 (MQ=255)
                                                                               gTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAgg                                                          <  1:577775/116‑1 (MQ=255)
                                                                                     ggCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTcc                             <  1:552593/139‑1 (MQ=255)
                                                                                        aaTTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGAt                                                                                                 >  1:566945/1‑68 (MQ=255)
                                                                                        aaTTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGAt                                                                                                 <  2:566945/68‑1 (MQ=255)
                                                                                         aTTTTTCGGGGATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTa                         >  1:356109/1‑139 (MQ=255)
                                                                                          tttttCGGGGATACTGCTCCAGGTAATTATTCGGTTa                                                                                                                              <  1:1565997/37‑1 (MQ=255)
                                                                                                ggggATACTGCTCCAGGTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAg                  >  2:1334326/1‑139 (MQ=255)
                                                                                                             cAGGTAATTATTCGGTTAGGAGTTAAGGCTGTtaca                                                                                                            <  2:64314/36‑1 (MQ=255)
                                                                                                             cAGGTAATTATTCGGTTAGGAGTTAAGGCTGTtaca                                                                                                            >  2:1636530/1‑36 (MQ=255)
                                                                                                             cAGGTAATTATTCGGTTAGGAGTTAAGGCTGTtaca                                                                                                            <  1:1636530/36‑1 (MQ=255)
                                                                                                             cAGGTAATTATTCGGTTAGGAGTTAAGGCTGTtaca                                                                                                            >  1:64314/1‑36 (MQ=255)
                                                                                                               ggTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTaa                        >  1:1332950/1‑118 (MQ=255)
                                                                                                               ggTAATTATTCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTaa                        <  2:1332950/118‑1 (MQ=255)
                                                                                                                    ttattCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATgag                                                                                              <  1:1527321/43‑1 (MQ=255)
                                                                                                                    ttattCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATgag                                                                                              >  2:1527321/1‑43 (MQ=255)
                                                                                                                    ttattCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCAtgtg                                                                                 <  2:1536523/56‑1 (MQ=255)
                                                                                                                    ttattCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCAtgtg                                                                                 >  1:1536523/1‑56 (MQ=255)
                                                                                                                    ttattCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGgaggg                                                           >  2:601155/1‑76 (MQ=255)
                                                                                                                    ttattCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGgaggg                                                           <  1:601155/78‑3 (MQ=255)
                                                                                                                    ttattCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTaac                       >  2:1396375/1‑114 (MQ=255)
                                                                                                                    ttattCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTaac                       <  1:1396375/114‑1 (MQ=255)
                                                                                                                    ttattCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTa  >  2:196889/1‑135 (MQ=255)
                                                                                                                    ttattCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTa  <  1:196889/135‑1 (MQ=255)
                                                                                                                     tattCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATgag                                                                                              <  2:253912/42‑1 (MQ=255)
                                                                                                                     tattCGGTTAGGAGTTAAGGCTGTTACACGGATTTGGATgag                                                                                              >  1:253912/1‑42 (MQ=255)
                                                                                                                            |                                                                                                                              
TGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGCTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTA  >  NC_000913/4499919‑4500169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A