Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
781 |
46.9 |
2231650 |
93.5% |
2086592 |
109.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
551,361 |
2 bp→AA |
intergenic (+29/+165) |
ybcF → / ← purK |
putative carbonate kinase/N5‑carboxyaminoimidazole ribonucleotide synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 551,361 | 0 | G | A | 92.9%
| 70.7
/ ‑1.1
| 28 | intergenic (+29/+166) | ybcF/purK | putative carbonate kinase/N5‑carboxyaminoimidazole ribonucleotide synthase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (12/14); total (13/15) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 |
| * | NC_000913 | 551,362 | 0 | C | A | 92.9%
| 80.2
/ 1.8
| 28 | intergenic (+30/+165) | ybcF/purK | putative carbonate kinase/N5‑carboxyaminoimidazole ribonucleotide synthase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (12/14); total (13/15) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.95e-01 |
TGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGG > NC_000913/551233‑551481
||
tGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGa < 2:198256/139‑1 (MQ=255)
aGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATc > 1:410191/1‑137 (MQ=255)
aGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATc < 2:410191/137‑1 (MQ=255)
cgTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACg > 2:704214/1‑105 (MQ=255)
cgTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACg < 1:704214/105‑1 (MQ=255)
gATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCAt < 1:96065/139‑1 (MQ=255)
ggCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATc < 2:833452/111‑1 (MQ=255)
ggCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATc > 1:833452/1‑111 (MQ=255)
cgAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGcac > 2:156645/1‑139 (MQ=255)
aTTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAAt < 2:575865/139‑1 (MQ=255)
aaGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCt < 1:156645/139‑1 (MQ=255)
gaCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatg < 1:856427/139‑1 (MQ=255)
cGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatgcg > 1:992006/1‑139 (MQ=255)
cgAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCtt < 1:513169/135‑1 (MQ=255)
cgAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCtt > 2:513169/1‑135 (MQ=255)
tGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAAt < 1:491186/96‑1 (MQ=38)
tGTAATCGTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAAt > 2:491186/1‑96 (MQ=38)
gTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAAt < 2:149926/78‑1 (MQ=255)
gTAGGCATTAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAAt > 1:149926/1‑78 (MQ=37)
tAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCt < 2:297483/85‑1 (MQ=255)
tAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCt > 1:297483/1‑85 (MQ=255)
tAGACATTTGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGCTGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAgg > 1:135203/1‑139 (MQ=255)
ttGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGc > 1:810075/1‑107 (MQ=255)
ttGTGCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGc < 2:810075/107‑1 (MQ=255)
tgCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt > 2:481655/1‑99 (MQ=255)
tgCCTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt < 1:481655/99‑1 (MQ=255)
cTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGTCGCATCCGGCAATTGGTGcac < 2:669210/65‑1 (MQ=25)
cTGATAAGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGcac > 1:669210/1‑65 (MQ=255)
||
TGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGG > NC_000913/551233‑551481
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
