Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,389,499	C→T	C190C (TGC→TGT)	ycjG →	L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,389,499	0	C	T	100.0%	114.3 / NA	36	C190C (TGC→TGT)	ycjG	L‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/18); total (18/18)

GCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGA  >  NC_000913/1389362‑1389632

gcgAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAA‑GGTTGGCGGCGCGTTGTCa                                                                                                                                     >  1:490907/1‑139 (MQ=255)

  gAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAg                                                                                                                                    <  2:1013029/139‑1 (MQ=255)

                 gAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTa                                                                                                                     >  2:302759/1‑139 (MQ=255)

                      tGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGt                                                                                                                <  1:275305/139‑1 (MQ=255)

                         aTAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCCACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGc                                                                                                             <  1:591560/139‑1 (MQ=255)

                            aCCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGAt                                                                                                          <  1:320832/139‑1 (MQ=255)

                             ccATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATg                                                                                                         >  1:467443/1‑139 (MQ=255)

                                  ttATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATTCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTCGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGa                                                                                                    >  2:331555/1‑139 (MQ=255)

                                  ttATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGc                                                                                                                  <  1:238323/125‑1 (MQ=255)

                                  ttATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGc                                                                                                                  >  2:238323/1‑125 (MQ=255)

                                   tATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGaa                                                                                                   <  1:69195/139‑1 (MQ=255)

                                          gAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGc                                                                                                                            >  1:791767/1‑107 (MQ=255)

                                          gAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGc                                                                                                                            <  2:791767/107‑1 (MQ=255)

                                          gAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGc                                                                                                                            <  2:383776/107‑1 (MQ=255)

                                          gAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGc                                                                                                                            >  1:383776/1‑107 (MQ=255)

                                             cGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                         <  2:940758/139‑1 (MQ=255)

                                             cGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                         <  2:467443/139‑1 (MQ=255)

                                             cGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                         <  1:302759/139‑1 (MQ=255)

                                                  ggtggCAATTCGCACAGCTGTGCCCGAAGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGCAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGAGATGCTTGAACAAGCGCTTCCTgcg                                                                                    >  1:719497/1‑139 (MQ=255)

                                                       cAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGa                                                                               <  1:343454/139‑1 (MQ=255)

                                                          ttCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCg                                                                                                            <  1:319811/107‑1 (MQ=255)

                                                          ttCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCg                                                                                                            >  2:319811/1‑107 (MQ=255)

                                                          ttCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGa                                                                            >  1:331526/1‑139 (MQ=255)

                                                          ttCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGa                                                                            >  2:805385/1‑139 (MQ=255)

                                                                             tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAAtttt                                                         >  1:9583/1‑139 (MQ=255)

                                                                                    cTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATc                                                  <  1:950589/139‑1 (MQ=255)

                                                                                                             ggCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAg                         <  1:123759/139‑1 (MQ=255)

                                                                                                             ggCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAg                         <  2:331526/139‑1 (MQ=255)

                                                                                                              gCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGt                        >  2:450368/1‑139 (MQ=255)

                                                                                                                             ggcggcGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTg                                                                                      <  2:434788/62‑1 (MQ=255)

                                                                                                                             ggcggcGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTg                                                                                      >  1:434788/1‑62 (MQ=255)

                                                                                                                                ggcgCGTTGTCAGCTATTGGTGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAg                         <  2:288102/120‑1 (MQ=255)

                                                                                                                                ggcgCGTTGTCAGCTATTGGTGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAg                         >  1:288102/1‑120 (MQ=255)

                                                                                                                                ggcgCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAAt      >  1:902533/1‑139 (MQ=255)

                                                                                                                                    cgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGa  >  2:395205/1‑139 (MQ=255)

                                                                                                                                    cgTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGa  >  1:169793/1‑139 (MQ=255)

GCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGA  >  NC_000913/1389362‑1389632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A