Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I1 R1 781 46.9 2231650 93.5% 2086592 109.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,765,794 (C)7→8 intergenic (‑18/‑124) yfjM ← / → rnlA CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,765,7871.C95.5% 143.7 / ‑0.0 44intergenic (‑11/‑131)yfjM/rnlACP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (18/24);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01

CTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAT  >  NC_000913/2765654‑2765905
                                                                                                                                      |                                                                                                                      
ctctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑ccccc                                                                                                                   <  2:1077319/139‑1 (MQ=255)
   tGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATATTGTGGACTG‑cccccccc                                                                                                                >  1:812914/1‑138 (MQ=255)
              tttCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATc                                                                                                      <  2:348350/139‑1 (MQ=255)
              tttCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATc                                                                                                      <  2:812914/139‑1 (MQ=255)
               ttCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGTGGTTACCGAAACGATGGACATCATCCCCCATTTTTTGGACTGCCCCCCCCGGTAGACGATcc                                                                                                     >  2:796548/1‑139 (MQ=255)
                        gAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCtata                                                                                            >  1:441623/1‑139 (MQ=255)
                          aTTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGATTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGt                                                                                          <  2:83588/139‑1 (MQ=255)
                               ttCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAg                                                                                     <  1:408063/139‑1 (MQ=255)
                               ttCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAg                                                                                     <  1:796548/139‑1 (MQ=255)
                                tCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAg                                                                                     <  1:1058904/138‑1 (MQ=255)
                                tCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAg                                                                                     >  2:1058904/1‑138 (MQ=255)
                                tCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                    >  1:544764/1‑139 (MQ=255)
                                   tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                 >  2:868003/1‑139 (MQ=255)
                                                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAg                                                                                     <  2:962689/107‑1 (MQ=255)
                                                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAg                                                                                     >  1:962689/1‑107 (MQ=255)
                                                                 aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCa                                                  >  1:493760/1‑139 (MQ=255)
                                                                    tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                               <  2:544764/139‑1 (MQ=255)
                                                                    tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                               <  1:374509/139‑1 (MQ=255)
                                                                           tGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa                                        >  2:842779/1‑139 (MQ=255)
                                                                            ggTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                       <  1:868003/139‑1 (MQ=255)
                                                                              tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGc                                                                                                  <  2:981694/79‑1 (MQ=255)
                                                                              tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGc                                                                                                  >  1:981694/1‑79 (MQ=255)
                                                                              tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                     <  1:909976/139‑1 (MQ=255)
                                                                              tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                     <  2:493760/139‑1 (MQ=255)
                                                                                     aGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGa                                                                                                           <  1:518992/63‑1 (MQ=255)
                                                                                     aGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGa                                                                                                           >  2:518992/1‑63 (MQ=255)
                                                                                             gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCTCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                    >  1:980903/1‑109 (MQ=255)
                                                                                             gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                    <  2:980903/109‑1 (MQ=255)
                                                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                    >  2:57694/1‑107 (MQ=255)
                                                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                    <  1:57694/107‑1 (MQ=255)
                                                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                    <  1:385576/139‑1 (MQ=255)
                                                                                                aCCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAg                                                                                     >  1:735505/1‑74 (MQ=255)
                                                                                                aCCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAg                                                                                     <  2:735505/74‑1 (MQ=255)
                                                                                                   gAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                <  2:372912/139‑1 (MQ=255)
                                                                                                                tcatcCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTa   <  1:842779/139‑1 (MQ=255)
                                                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                     >  2:435397/1‑104 (MQ=255)
                                                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                     <  1:435397/104‑1 (MQ=255)
                                                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAt  >  2:684413/1‑139 (MQ=255)
                                                                                                                  atcCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                             <  2:951666/95‑1 (MQ=255)
                                                                                                                  atcCCCCATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                             >  1:951666/1‑95 (MQ=255)
                                                                                                                    cccccATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAg                                                         <  1:729428/81‑1 (MQ=255)
                                                                                                                    cccccATTTTGTGGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAg                                                         >  2:729428/1‑81 (MQ=255)
                                                                                                                    cccccATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCa             <  1:547229/124‑1 (MQ=255)
                                                                                                                    cccccATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCa             >  2:547229/1‑124 (MQ=255)
                                                                                                                               tgGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAg                                                         <  2:113552/70‑1 (MQ=255)
                                                                                                                               tgGACTGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAg                                                         >  1:113552/1‑70 (MQ=255)
                                                                                                                                      |                                                                                                                      
CTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAT  >  NC_000913/2765654‑2765905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A