Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,304,455	G→A	336 (TAG→TAA)	yhbW →	putative luciferase‑like monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,304,455	0	G	A	100.0%	156.9 / NA	49	336 (TAG→TAA)	yhbW	putative luciferase‑like monooxygenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/25); total (24/25)

TCGATCCTGCGCGAAACCGACGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGC  >  NC_000913/3304336‑3304588

tCGATCCTGCGCGAAACCGACGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAg                                                                                                                    >  2:520432/1‑139 (MQ=255)

              aaCCGACGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTg                                                                                                      >  1:1063288/1‑139 (MQ=255)

              aaCCAACGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTg                                                                                                      >  2:912420/1‑139 (MQ=255)

                   aCGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAAc                                                                                                 >  1:435913/1‑139 (MQ=255)

                    cGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                                <  1:281073/139‑1 (MQ=255)

                      ccGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCt                                                                                              <  1:848329/139‑1 (MQ=255)

                         aTGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTc                                                                                           <  2:408852/139‑1 (MQ=255)

                         aTGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTc                                                                                           <  1:869946/139‑1 (MQ=255)

                          tGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCggg                                                                                                                       >  2:756007/1‑110 (MQ=255)

                          tGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCggg                                                                                                                       <  1:756007/110‑1 (MQ=255)

                          tGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                                >  2:518713/1‑133 (MQ=255)

                          tGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                                <  1:518713/133‑1 (MQ=255)

                                tATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATa                                                                                    <  2:583167/139‑1 (MQ=255)

                                    gTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                                >  2:620118/1‑123 (MQ=255)

                                    gTCAACGGACAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                                <  1:620118/123‑1 (MQ=255)

                                         cGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCgg                                                                                                                        <  2:842627/94‑1 (MQ=255)

                                         cGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCgg                                                                                                                        >  1:842627/1‑94 (MQ=255)

                                                     cGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                                >  1:1044464/1‑106 (MQ=255)

                                                     cGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                                <  2:1044464/106‑1 (MQ=255)

                                                     cGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCAt                                                               >  1:640090/1‑139 (MQ=255)

                                                        ccaccaGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAg                                                            <  1:487978/139‑1 (MQ=255)

                                                          accaGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGCTAAGCGAAGCGCATCAGGc                                                          <  1:741675/139‑1 (MQ=255)

                                                          accaGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGc                                                          <  1:520432/139‑1 (MQ=255)

                                                          accaGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGc                                                          <  2:11671/139‑1 (MQ=255)

                                                           ccaGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGAt                                                                            <  1:230738/120‑1 (MQ=255)

                                                           ccaGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGAt                                                                            >  2:230738/1‑120 (MQ=255)

                                                              ggCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATtg                                                      >  2:706921/1‑139 (MQ=255)

                                                                  cgGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTa                                                  <  2:640090/139‑1 (MQ=255)

                                                                               ttGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATg                                     <  1:706921/139‑1 (MQ=255)

                                                                                  aGCTAGCGATGGATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCg                                  >  2:322819/1‑139 (MQ=255)

                                                                                    cTGGCGATGCATGTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAGGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGa                                >  1:746422/1‑139 (MQ=255)

                                                                                              atgTTAAGGAAGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAgcgc                                                                 >  2:1074634/1‑96 (MQ=255)

                                                                                              atgTTAAGGAAGAGTTGTTGAGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAgcgc                                                                 <  1:1074634/96‑1 (MQ=255)

                                                                                                       aaGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                                >  2:648795/1‑56 (MQ=255)

                                                                                                       aaGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                                <  1:648795/56‑1 (MQ=255)

                                                                                                       aaGAGTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt             >  2:79364/1‑139 (MQ=255)

                                                                                                          agTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                                >  1:182381/1‑53 (MQ=255)

                                                                                                          agTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACg                                                                                                <  2:182381/53‑1 (MQ=255)

                                                                                                          agTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTc                                   <  1:440265/114‑1 (MQ=255)

                                                                                                          agTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTc                                   >  2:440265/1‑114 (MQ=255)

                                                                                                          agTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGc                     <  2:57619/128‑1 (MQ=255)

                                                                                                          agTTGTTGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGc                     >  1:57619/1‑128 (MQ=255)

                                                                                                           gttgttGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa         >  1:262014/1‑139 (MQ=255)

                                                                                                           gttgttGGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa         >  1:510037/1‑139 (MQ=255)

                                                                                                              gttgGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCg                      <  1:637018/123‑1 (MQ=255)

                                                                                                              gttgGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATacac      <  2:435913/139‑1 (MQ=255)

                                                                                                              gttgGGATAATGTGTCTGAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAATCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCg                      >  2:637018/1‑123 (MQ=255)

                                                                                                               ttgGGATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACAcc     >  1:227537/1‑139 (MQ=255)

                                                                                                                  ggATAATGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGc  <  1:322819/139‑1 (MQ=255)

TCGATCCTGCGCGAAACCGACGCCGATGAGATTATGGTCAACGGGCAGATTTTCGACCACCAGGCGCGGCTGCATTCGTTTGAGCTGGCGATGGATGTTAAGGAAGAGTTGTTGGGATAGTGTGTCTTAACGCGGGAAGCCTTATCCGAGCTGGCAACGCTGTCCTACATAGACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGC  >  NC_000913/3304336‑3304588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A