Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I1 R1 750 51.0 2595496 93.6% 2429384 106.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,351,197 C→T intergenic (‑158/+210) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,1970CT100.0% 181.1 / NA 58intergenic (‑158/+210)fabI/ycjDenoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/30);  total (28/30)

TGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACC  >  NC_000913/1351065‑1351330
                                                                                                                                    |                                                                                                                                     
tgttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCtttt                                                                                                                                 >  1:493906/1‑139 (MQ=255)
  ttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTcc                                                                                                                               >  2:51019/1‑139 (MQ=255)
    gtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTcccc                                                                                                                             <  1:505154/139‑1 (MQ=255)
       ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTcccc                                                                                                                             >  1:793798/1‑136 (MQ=255)
       ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTcccc                                                                                                                             <  2:793798/136‑1 (MQ=255)
           aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCt                                                                                                                      >  2:1197187/1‑139 (MQ=255)
                          gtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                                       <  2:192860/139‑1 (MQ=255)
                                 tAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                                <  1:51019/139‑1 (MQ=255)
                                           tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                      <  2:493906/139‑1 (MQ=255)
                                             cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAAcc                                                                                                                  <  2:1173172/109‑1 (MQ=255)
                                             cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAAcc                                                                                                                  >  1:1173172/1‑109 (MQ=255)
                                             cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                                <  1:318061/127‑1 (MQ=255)
                                             cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                                >  2:318061/1‑127 (MQ=255)
                                                            ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                         >  1:763613/1‑103 (MQ=255)
                                                            ttattaTAATCAACCTGGCTGCGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                         <  2:763613/103‑1 (MQ=255)
                                                              attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCct                                                                                                                <  1:380489/94‑1 (MQ=255)
                                                              attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCct                                                                                                                >  2:380489/1‑94 (MQ=255)
                                                                      cAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCt                                                                                                                            <  2:679125/74‑1 (MQ=255)
                                                                      cAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCt                                                                                                                            >  1:679125/1‑74 (MQ=255)
                                                                         ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                              <  2:643759/101‑1 (MQ=255)
                                                                         ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                              >  1:643759/1‑101 (MQ=255)
                                                                         ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                        >  2:1031868/1‑139 (MQ=255)
                                                                                   aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTccc                                                                                                                              >  1:1117206/1‑59 (MQ=255)
                                                                                   aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTccc                                                                                                                              <  2:1117206/59‑1 (MQ=255)
                                                                                   aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACCGGCTCATa                                              >  1:190220/1‑139 (MQ=255)
                                                                                        cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTCTTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATaa                                             <  1:795048/135‑2 (MQ=255)
                                                                                        cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATAc                                             >  2:795048/1‑135 (MQ=255)
                                                                                                gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                      >  1:730678/1‑86 (MQ=255)
                                                                                                gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                      <  2:461616/86‑1 (MQ=255)
                                                                                                gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                      >  1:461616/1‑86 (MQ=255)
                                                                                                gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                      <  2:730678/86‑1 (MQ=255)
                                                                                                gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAccc                                 >  2:664086/1‑139 (MQ=255)
                                                                                                 ccAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGt                                                                       >  1:305657/1‑100 (MQ=255)
                                                                                                 ccAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGt                                                                       <  2:305657/100‑1 (MQ=255)
                                                                                                 ccAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCt                                >  1:632800/1‑139 (MQ=255)
                                                                                                 ccAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCt                                >  1:430894/1‑139 (MQ=255)
                                                                                                  cAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCCCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCtt                               >  2:1256908/1‑139 (MQ=255)
                                                                                                     gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAAcc                                                                                                                  >  1:424802/1‑53 (MQ=255)
                                                                                                     gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAAcc                                                                                                                  <  2:424802/53‑1 (MQ=255)
                                                                                                     gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                                  >  2:883252/1‑85 (MQ=255)
                                                                                                     gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                                  <  1:883252/85‑1 (MQ=255)
                                                                                                        cGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaaca                                                      <  2:1253496/110‑1 (MQ=255)
                                                                                                        cGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaaca                                                      >  1:1253496/1‑110 (MQ=255)
                                                                                                          aCCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                        <  1:722295/106‑1 (MQ=255)
                                                                                                          aCCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                        >  2:722295/1‑106 (MQ=255)
                                                                                                          aCCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg                       <  2:952896/139‑1 (MQ=255)
                                                                                                               aGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                         >  2:356374/1‑52 (MQ=255)
                                                                                                               aGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                         <  1:356374/52‑1 (MQ=255)
                                                                                                                    gCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa             <  2:632800/139‑1 (MQ=255)
                                                                                                                    gCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa             <  1:504722/139‑1 (MQ=255)
                                                                                                                    gCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa             <  1:1197187/139‑1 (MQ=255)
                                                                                                                    gCTGCGGCAGGGGGGGTGCTTTTCCCCCCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa             <  1:1256908/139‑1 (MQ=255)
                                                                                                                     cTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa             <  2:1028878/138‑1 (MQ=255)
                                                                                                                     cTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa             >  1:1028878/1‑138 (MQ=255)
                                                                                                                      tGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                                     >  1:901935/1‑65 (MQ=255)
                                                                                                                      tGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                                     <  2:901935/65‑1 (MQ=255)
                                                                                                                             gggggggTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGa    <  2:430894/139‑1 (MQ=255)
                                                                                                                               gggggTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAcc  <  1:664086/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
TGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACC  >  NC_000913/1351065‑1351330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A