Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,021,501	G→A	138 (TAG→TAA)	fliN →	flagellar motor switching and energizing component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,021,501	0	G	A	100.0%	132.5 / NA	42	138 (TAG→TAA)	fliN	flagellar motor switching and energizing component
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/20); total (22/20)

AACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAAC  >  NC_000913/2021371‑2021624

agccACTGGCTATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGGCTGAGCCGTTAATGATGAAt                                                                                                                     <  2:760775/137‑1 (MQ=255)

     caggATATTCTGATCAACGGTTACTTAATCGCCCAGGGCGAAGTGGTGGTCGTTACCGATAAATATGGCGTGCGGATCATCGATATCATTACTCCGTCTTAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCa                                                                                                                <  2:853595/137‑1 (MQ=255)

                gATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACg                                                                                                              >  2:336963/1‑130 (MQ=255)

                gATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACg                                                                                                              <  1:336963/130‑1 (MQ=255)

                  tCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAAtg                                                                                                                           >  2:134551/1‑115 (MQ=255)

                   cAACGGTTATTTAATCGCACAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACtgtacctg                                                                                                  >  1:853595/1‑134 (MQ=255)

                    aaCGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATc                                                                                                 >  2:1017944/1‑139 (MQ=255)

                         ttATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCcg                                                                                            >  1:231285/1‑139 (MQ=255)

                                            gAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGcc                                                                         <  1:1017944/139‑1 (MQ=255)

                                            gAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGcc                                                                         <  2:1187836/139‑1 (MQ=255)

                                              aGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTa                                                                                                           >  2:1259112/1‑103 (MQ=255)

                                              aGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTa                                                                                                           <  1:1259112/103‑1 (MQ=255)

                                                   tggtCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCActgctg                                                                  >  1:403628/1‑139 (MQ=255)

                                                     gtCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAAc                                                                                                                  >  1:1067357/1‑89 (MQ=255)

                                                     gtCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAAc                                                                                                                  <  2:1067357/89‑1 (MQ=255)

                                                                  aaTATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCa                                                   <  2:403628/139‑1 (MQ=255)

                                                                           tGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCa                                                                                                                >  1:546149/1‑69 (MQ=255)

                                                                           tGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCa                                                                                                                <  2:546149/69‑1 (MQ=255)

                                                                           tGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTTCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGcc                                          >  1:1215375/1‑139 (MQ=255)

                                                                              ggATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCActgc                                                                    <  2:679921/110‑1 (MQ=255)

                                                                              ggATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCActgc                                                                    >  1:679921/1‑110 (MQ=255)

                                                                                  cACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACg                                                                                                              <  2:367849/64‑1 (MQ=255)

                                                                                  cACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACg                                                                                                              >  1:367849/1‑64 (MQ=255)

                                                                                       atatCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAAt                                                                                                                     >  2:1090096/1‑52 (MQ=255)

                                                                                       atatCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAAt                                                                                                                     <  1:1090096/52‑1 (MQ=255)

                                                                                         atCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAAtg                                                                                                                           <  2:794711/44‑1 (MQ=255)

                                                                                            aTTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAatgatg                                                                                                                        >  1:930493/1‑44 (MQ=255)

                                                                                            aTTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAatgatg                                                                                                                        <  2:930493/44‑1 (MQ=255)

                                                                                               aCTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAACGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGTTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCgc                      <  1:610378/139‑1 (MQ=255)

                                                                                                   cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACg                                                                                                              <  1:1054739/47‑1 (MQ=255)

                                                                                                   cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACg                                                                                                              >  2:1054739/1‑47 (MQ=255)

                                                                                                   cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACt                                                                                                         <  1:1121452/52‑1 (MQ=255)

                                                                                                   cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACt                                                                                                         >  2:1121452/1‑52 (MQ=255)

                                                                                                   cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCgctgct                  >  1:302202/1‑139 (MQ=255)

                                                                                                   cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCgctgct                  >  1:925192/1‑139 (MQ=255)

                                                                                                   cGTCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCgctgct                  >  2:944505/1‑139 (MQ=255)

                                                                                                     tCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCa                                                                        >  1:890501/1‑83 (MQ=255)

                                                                                                     tCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCa                                                                        <  2:890501/83‑1 (MQ=255)

                                                                                                     tCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGc                                           >  1:936083/1‑112 (MQ=255)

                                                                                                     tCTGAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGc                                           <  2:936083/112‑1 (MQ=255)

                                                                                                        gAGCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctg             >  2:660749/1‑139 (MQ=255)

                                                                                                          gCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctggc           <  2:302202/139‑1 (MQ=255)

                                                                                                          gCGAATGCGCCGCCTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCctggc           <  1:531949/139‑1 (MQ=255)

                                                                                                                   ccgccTGAGCCGTTAATGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAAc  >  1:1011495/1‑139 (MQ=255)

AACCACTGGATATTCTGATCAACGGTTATTTAATCGCCCAGGGCGAAGTGGTGGTCGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACTCCGTCTGAGCGAATGCGCCGCCTGAGCCGTTAGTGATGAATAACCACGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGCGCCACTGCTGCAGGTGAGCGGCGCACTCATCGCCATTATTGCCCTGATCCTCGCTGCTGCCTGGCTGGTAAAAC  >  NC_000913/2021371‑2021624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A