Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,029,729	(C)_6→5	coding (191/921 nt)	yedA →	amino acid exporter for phenylalanine, threonine

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,029,724	0	C	.	100.0%	116.3 / NA	28	coding (186/921 nt)	yedA	amino acid exporter for phenylalanine, threonine
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (16/12); total (16/12)

ATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTG  >  NC_000913/2029588‑2029856

atatCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTccc                                                                                                                                    >  2:301240/1‑139 (MQ=255)

         ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGGCACAAACT‑CCCCCGCTACGt                                                                                                                          >  1:1090542/1‑139 (MQ=255)

         ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGt                                                                                                                          >  2:642157/1‑139 (MQ=255)

         ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGt                                                                                                                          >  1:304695/1‑139 (MQ=255)

         ggggCTCAACCTATTTTGTCATTCGGATTGGCGAGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGCCATTTTTGTTACTGCGCGGGACCAAACT‑CCCCCGCTACGt                                                                                                                          >  2:1060710/1‑139 (MQ=255)

                 aCCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCgctgct                                                                                                                  <  1:301240/139‑1 (MQ=255)

                              ttCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTg                                                                                                     >  1:546238/1‑139 (MQ=255)

                                cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGAt                                                                                                   <  2:304695/139‑1 (MQ=255)

                                cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGAt                                                                                                   >  2:1002314/1‑139 (MQ=255)

                                cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGAt                                                                                                   >  1:968289/1‑139 (MQ=255)

                                cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGAt                                                                                                   <  1:642157/139‑1 (MQ=255)

                                   aTTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                <  2:546238/139‑1 (MQ=255)

                                        cGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGt                                                                                           >  2:1129759/1‑139 (MQ=255)

                                                 cTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                  >  1:471015/1‑139 (MQ=255)

                                                 cTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                  >  1:792477/1‑139 (MQ=255)

                                                    gCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCg                                                                                                                       >  1:1293778/1‑99 (MQ=255)

                                                    gCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCg                                                                                                                       <  2:1293778/99‑1 (MQ=255)

                                                     ccTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGt                                                                              <  1:339130/139‑1 (MQ=255)

                                                     ccTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGt                                                                              <  1:1129759/139‑1 (MQ=255)

                                                      cTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                <  1:1046549/120‑1 (MQ=255)

                                                      cTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                >  2:1046549/1‑120 (MQ=255)

                                                                     cgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTg                                                              >  1:145249/1‑139 (MQ=255)

                                                                     cgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTg                                                              >  2:659915/1‑139 (MQ=255)

                                                                                    tGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACAt                                               <  2:624513/139‑1 (MQ=255)

                                                                                             gTATTTTATTGCTGGCATTTTTGCTACTGCGCGGGCACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGtt                                      <  2:1090542/139‑1 (MQ=255)

                                                                                                ttttATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTg                                                                                                 <  1:488286/77‑1 (MQ=255)

                                                                                                ttttATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTg                                                                                                 >  2:488286/1‑77 (MQ=255)

                                                                                                          ggCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcg                         >  2:140552/1‑139 (MQ=255)

                                                                                                                         gcgcGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                 <  2:1017581/132‑1 (MQ=255)

                                                                                                                         gcgcGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                 >  1:1017581/1‑132 (MQ=255)

                                                                                                                                 acaAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGACATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTg  <  2:968289/139‑1 (MQ=255)

ATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTG  >  NC_000913/2029588‑2029856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A