Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
750 |
51.0 |
2595496 |
93.6% |
2429384 |
106.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,188,043 |
(C)6→7 |
coding (372/1035 nt) |
yehA ← |
putative fimbrial‑like adhesin protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,188,037 | 1 | . | C | 100.0%
| 111.1
/ NA
| 33 | G126G (GGC→GGG) | yehA | putative fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (19/14); total (19/14) |
CACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCG‑CCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAA > NC_000913/2187916‑2188158
|
cacaTGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACAt > 1:1256160/1‑139 (MQ=255)
acaTGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACAtt < 1:72439/139‑1 (MQ=255)
gCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAAccgccg > 1:741870/1‑103 (MQ=255)
gCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAAccgccg < 2:741870/103‑1 (MQ=255)
gCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGc > 1:1230958/1‑139 (MQ=255)
gCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCG‑cccc > 2:713272/1‑107 (MQ=255)
gCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCG‑cccc < 1:713272/107‑1 (MQ=255)
caGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACCCCATTAATCATCACTCAACCGCCGCCCCCCCGCAGAAACATTCAc < 2:747916/109‑1 (MQ=255)
caGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCAc > 1:747916/1‑109 (MQ=255)
caGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGt > 1:845823/1‑139 (MQ=255)
aGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCgccg‑cc < 1:1162833/89‑1 (MQ=255)
aGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCgccg‑cc > 2:1162833/1‑89 (MQ=255)
tAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCAc > 1:1123400/1‑133 (MQ=255)
tAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCAc < 2:1123400/133‑1 (MQ=255)
gTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTa > 1:364410/1‑129 (MQ=255)
gTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTa < 2:364410/129‑1 (MQ=255)
aCTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCa > 2:241995/1‑139 (MQ=255)
aCTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAAGCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCa > 1:970811/1‑139 (MQ=255)
cGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCACAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTgg > 1:343381/1‑139 (MQ=255)
cTCCCGCGTGTCGATTATGAAGCACTGGATACTGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGaaa < 2:874352/139‑1 (MQ=255)
cTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGaaa > 1:872276/1‑139 (MQ=255)
gcgTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGt < 2:872276/139‑1 (MQ=255)
gcgTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCACAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGt < 2:343381/139‑1 (MQ=255)
cgTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTc > 2:1025787/1‑139 (MQ=255)
ggATAATGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGAt > 2:1294243/1‑139 (MQ=255)
aTGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAAc < 2:993358/89‑1 (MQ=255)
aTGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAAc > 1:993358/1‑89 (MQ=255)
tGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCt > 2:185278/1‑114 (MQ=255)
tGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCt < 1:185278/114‑1 (MQ=255)
tGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACg > 2:701348/1‑129 (MQ=255)
tGTCGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACg < 1:701348/129‑1 (MQ=255)
cGCAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGttttt > 2:770357/1‑139 (MQ=255)
cAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGAtgttgt < 1:726226/133‑1 (MQ=255)
cAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGAtgttgt > 2:726226/1‑133 (MQ=255)
cAACACCATTAATCATCACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTAt > 2:474193/1‑139 (MQ=255)
atcatcACTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCa < 1:474193/139‑1 (MQ=255)
tcatcaCTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCaa < 1:1294243/139‑1 (MQ=255)
cTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCAc < 2:52770/59‑1 (MQ=39)
cTGAACCGCCGCCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCAc > 1:52770/1‑59 (MQ=39)
|
CACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCATCACTGAACCGCCG‑CCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAA > NC_000913/2187916‑2188158
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
