Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,274,166	A→G	K601K (AAA→AAG)	yejA →	microcin C ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,274,166	0	A	G	100.0%	223.2 / NA	61	K601K (AAA→AAG)	yejA	microcin C ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (32/29); total (32/29)

TGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCAT  >  NC_000913/2274032‑2274294

tgGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAgg                                                                                                                              <  2:210382/139‑1 (MQ=255)

        ggCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaa                                                                                                                      >  1:1260859/1‑138 (MQ=255)

          cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaaat                                                                                                                    >  2:1026094/1‑136 (MQ=255)

          cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAAt                                                                                                                    >  1:643922/1‑139 (MQ=255)

          cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAAt                                                                                                                    >  2:17030/1‑139 (MQ=255)

                    tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTAc                                                                                                          >  2:720167/1‑139 (MQ=255)

                             gtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGgcg                                                                                                               >  1:576274/1‑125 (MQ=255)

                             gtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGgcg                                                                                                               <  2:576274/125‑1 (MQ=255)

                                ggATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGt                                                                                              <  2:720687/139‑1 (MQ=255)

                                         ctcCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTg                                                                                     <  1:17030/139‑1 (MQ=255)

                                           cccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                   <  2:1260859/139‑1 (MQ=255)

                                              aGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGAt                                                                                <  1:1026094/139‑1 (MQ=255)

                                                 cggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                             <  1:444584/139‑1 (MQ=255)

                                                     cgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACa                                                                         >  2:1248579/1‑139 (MQ=255)

                                                            cccATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                                                                  <  1:629946/139‑1 (MQ=255)

                                                                   ataGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTAt                                                           <  2:643922/139‑1 (MQ=255)

                                                                          cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                                    >  2:30132/1‑139 (MQ=255)

                                                                          cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                                    >  2:942870/1‑139 (MQ=255)

                                                                           ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaaat                                                                                                                    <  2:480578/74‑4 (MQ=255)

                                                                           ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaaat                                                                                                                    >  1:480578/1‑71 (MQ=255)

                                                                           ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGa                                                                                 >  2:158651/1‑109 (MQ=255)

                                                                           ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGa                                                                                 <  1:158651/109‑1 (MQ=255)

                                                                           ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                             <  2:424844/113‑1 (MQ=255)

                                                                           ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                             >  1:424844/1‑113 (MQ=255)

                                                                           ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTAt                                                           >  1:1076909/1‑131 (MQ=255)

                                                                           ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTAt                                                           <  2:1076909/131‑1 (MQ=255)

                                                                           ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCa                                                   >  1:1218741/1‑139 (MQ=255)

                                                                            gTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGagag                                                                                                                         <  1:1013641/68‑1 (MQ=255)

                                                                            gTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGagag                                                                                                                         >  2:1013641/1‑68 (MQ=255)

                                                                            gTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAATAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCaa                                                  >  2:755478/1‑139 (MQ=255)

                                                                                  aTACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACttttt                                            >  1:929606/1‑139 (MQ=255)

                                                                                       tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCg                                       >  1:759737/1‑139 (MQ=255)

                                                                                         gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATc                                                          >  1:1236705/1‑118 (MQ=255)

                                                                                         gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATc                                                          <  2:1236705/118‑1 (MQ=255)

                                                                                         gtggtATGACGTCAATAAAGCGACCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                                                                  <  2:1055109/110‑1 (MQ=255)

                                                                                         gtggtATGACGTCAATAAAGCGACCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                                                                  >  1:1055109/1‑110 (MQ=255)

                                                                                             tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCaaa                                 >  1:850156/1‑139 (MQ=255)

                                                                                                   gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGga                                                                                                                            <  2:379582/42‑1 (MQ=255)

                                                                                                   gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGga                                                                                                                            >  1:379582/1‑42 (MQ=255)

                                                                                                   gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGAt                                                                                                     >  1:638447/1‑65 (MQ=255)

                                                                                                   gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGAt                                                                                                     <  2:638447/65‑1 (MQ=255)

                                                                                                   gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGt                                                                                              <  1:535107/72‑1 (MQ=255)

                                                                                                   gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGt                                                                                              >  2:535107/1‑72 (MQ=255)

                                                                                                      aaTAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTg                        >  1:1275734/1‑139 (MQ=255)

                                                                                                          aaGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcgg                    <  1:720167/139‑1 (MQ=255)

                                                                                                           aGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggt                   >  1:221788/1‑139 (MQ=255)

                                                                                                           aGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggt                   <  1:942870/139‑1 (MQ=255)

                                                                                                             cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATgg                                                                   >  1:826046/1‑89 (MQ=255)

                                                                                                             cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATgg                                                                   <  2:826046/89‑1 (MQ=255)

                                                                                                             cGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtcc                 >  1:175416/1‑139 (MQ=255)

                                                                                                               gCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtccgg               <  1:561940/139‑1 (MQ=255)

                                                                                                                ccgaaCTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCa                                                      >  2:646299/4‑99 (MQ=255)

                                                                                                                ccgaaCTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCa                                                      <  1:646299/96‑1 (MQ=255)

                                                                                                                ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGgcgc                                                                                                              >  1:1106113/1‑43 (MQ=255)

                                                                                                                ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGgcgc                                                                                                              <  2:1106113/43‑1 (MQ=255)

                                                                                                                ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                                                                  >  2:573242/1‑87 (MQ=255)

                                                                                                                ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                                                                  <  1:573242/87‑1 (MQ=255)

                                                                                                                        ccGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAgg      <  2:175416/139‑1 (MQ=255)

                                                                                                                            ccGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCAt  <  2:221788/139‑1 (MQ=255)

                                                                                                                             cGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                                    >  1:1166206/1‑88 (MQ=255)

                                                                                                                             cGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                                    <  2:1166206/88‑1 (MQ=255)

TGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCAT  >  NC_000913/2274032‑2274294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A