Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	371,542	C→T	Q102* (CAA→TAA)	mhpC →	2‑hydroxy‑6‑ketonona‑2,4‑dienedioic acid hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	371,542	0	C	T	100.0%	103.3 / NA	33	Q102* (CAA→TAA)	mhpC	2‑hydroxy‑6‑ketonona‑2,4‑dienedioic acid hydrolase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/14); total (19/14)

TGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATG  >  NC_000913/371420‑371670

tGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCaa                                                                                                                  <  1:253120/139‑1 (MQ=255)

     gagGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATcc                                                                                                             <  1:378372/139‑1 (MQ=255)

                tCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGc                                                                                                  <  2:130482/139‑1 (MQ=255)

                      gATCCTGCTGGATTGTCCGGGATGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCg                                                                                            >  1:807269/1‑139 (MQ=255)

                                 aTTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGTGGGGCCa                                                                                 <  1:119202/139‑1 (MQ=255)

                                 aTTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCa                                                                                 >  1:618424/1‑139 (MQ=255)

                                 aTTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCa                                                                                 <  1:842792/139‑1 (MQ=255)

                                  ttGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATc                                                                                                              <  1:555557/109‑1 (MQ=255)

                                  ttGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATc                                                                                                              >  2:555557/1‑109 (MQ=255)

                                     tCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGt                                                                             >  2:522596/1‑139 (MQ=255)

                                         ggTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTTGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCtg                                                                         <  2:807269/139‑1 (MQ=255)

                                          gTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCtgt                                                                        >  2:54579/1‑139 (MQ=255)

                                                                     aTAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAgcg                                             <  2:618424/139‑1 (MQ=255)

                                                                           gTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCgg                                       >  1:371160/1‑139 (MQ=255)

                                                                           gTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCgg                                       >  1:613017/1‑139 (MQ=255)

                                                                            ttCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGc                                      >  2:806733/1‑139 (MQ=255)

                                                                              cgcgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCaa                                                                                                                  >  1:148361/1‑61 (MQ=255)

                                                                              cgcgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCaa                                                                                                                  <  2:148361/61‑1 (MQ=255)

                                                                              cgcgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCaa                                    >  1:951129/1‑139 (MQ=255)

                                                                               gcgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCaaa                                   >  2:593623/1‑139 (MQ=255)

                                                                                cgATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAAc                                  >  1:234202/1‑139 (MQ=255)

                                                                                             aTGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATgggg                                                                                      >  2:407239/1‑74 (MQ=255)

                                                                                             aTGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATgggg                                                                                      <  1:407239/74‑1 (MQ=255)

                                                                                             aTGCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATggg                     <  2:951129/139‑1 (MQ=255)

                                                                                               gCACGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGggcg                   <  2:613017/139‑1 (MQ=255)

                                                                                                 aCGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGggcggc                 >  2:1035571/1‑139 (MQ=255)

                                                                                                 aCGAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGggcggc                 >  1:488560/1‑139 (MQ=255)

                                                                                                   gAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATggg                     <  2:1044760/133‑1 (MQ=255)

                                                                                                   gAATCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATggg                     >  1:1044760/1‑133 (MQ=255)

                                                                                                     aTCCTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTa             >  1:81374/1‑139 (MQ=255)

                                                                                                       ccTGAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACg           <  2:488560/139‑1 (MQ=255)

                                                                                                          gAAAAGCGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCGGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGggc        >  1:641258/1‑139 (MQ=255)

                                                                                                                cGTGGTGGATTAACTGGATATCGCCAAAATCCACCTGATGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCAGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATg  >  2:69740/1‑139 (MQ=255)

TGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATG  >  NC_000913/371420‑371670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A