Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	379,606	C→T	92 (TAG→TAA)	frmR ←	regulator protein that represses frmRAB operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	379,606	0	C	T	100.0%	107.2 / NA	34	92 (TAG→TAA)	frmR	regulator protein that represses frmRAB operon
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/13); total (21/13)

ATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGC  >  NC_000913/379477‑379740

aTTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGa                                                                                                                               >  2:799913/1‑139 (MQ=255)

     cACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGc                                                                                                                          >  1:370675/1‑139 (MQ=255)

      aCTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCa                                                                                                                         >  1:279704/1‑139 (MQ=255)

          cACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGaa                                                                                                                     >  1:423022/1‑139 (MQ=255)

           aCCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                    <  2:423022/139‑1 (MQ=255)

              ttttttCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAg                                                                                                                 <  2:378660/139‑1 (MQ=255)

                  ttCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCa                                                                                                             <  2:370675/139‑1 (MQ=255)

                          tgCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTgtc                                                                                                     >  1:68271/1‑139 (MQ=255)

                             aaCGTCAATTTCAACTATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTTAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTgtcgtc                                                                                                  <  1:97423/139‑1 (MQ=255)

                             aaCGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTgtcgtc                                                                                                  <  1:89919/139‑1 (MQ=255)

                                 tCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACg                                                                                              >  1:33308/1‑139 (MQ=255)

                                              tttCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACtt                                                                                 <  2:279704/139‑1 (MQ=255)

                                                   aGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCgg                                                                            <  2:280276/139‑1 (MQ=255)

                                                   aGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCgg                                                                            <  1:198145/139‑1 (MQ=255)

                                                        tttACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGACTGTa                                                                       >  1:657089/1‑139 (MQ=255)

                                                                                agcaCGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTc                                               >  1:445426/1‑139 (MQ=255)

                                                                                             aTCTCTCGCACTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCtt                                  >  1:708079/1‑139 (MQ=255)

                                                                                              tctctcGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCttt                                 >  1:266818/1‑139 (MQ=255)

                                                                                                  tcGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAg                             <  2:68271/139‑1 (MQ=255)

                                                                                                    gCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCa                           <  2:266818/139‑1 (MQ=255)

                                                                                                     ctctTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCGATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCAc                          >  2:64623/1‑139 (MQ=255)

                                                                                                         tCCTTAATATGGTAACATATTCAGTTATTTAAGATAGACACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCt                      <  1:619233/139‑1 (MQ=255)

                                                                                                                  tGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGc             >  1:924977/1‑139 (MQ=255)

                                                                                                                    gTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGa                                                                                            <  1:781815/58‑1 (MQ=255)

                                                                                                                    gTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGa                                                                                            >  2:781815/1‑58 (MQ=255)

                                                                                                                    gTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGccc           >  1:970364/1‑139 (MQ=255)

                                                                                                                     tAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTa                                                                       >  2:316370/1‑78 (MQ=255)

                                                                                                                     tAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTa                                                                       <  1:316370/78‑1 (MQ=255)

                                                                                                                     tAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCa          >  2:483221/1‑139 (MQ=255)

                                                                                                                      aaTAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCAt         >  1:162505/1‑139 (MQ=255)

                                                                                                                        tAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTa       >  2:573352/1‑139 (MQ=255)

                                                                                                                          gATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGc     >  1:1032144/1‑139 (MQ=255)

                                                                                                                          gATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGc     >  1:779469/1‑139 (MQ=255)

                                                                                                                             tCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCcgc  >  2:72511/1‑139 (MQ=255)

ATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGC  >  NC_000913/379477‑379740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A