Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	477,025	G→A	118 (TAG→TAA)	ybaA →	DUF1428 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	477,025	0	G	A	100.0%	96.7 / NA	31	118 (TAG→TAA)	ybaA	DUF1428 family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/14); total (17/14)

CTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCA  >  NC_000913/476892‑477146

cTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGc                                                                                                                      >  2:545929/1‑139 (MQ=255)

              cgcgACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTgc                                                                                                        <  1:464391/139‑1 (MQ=255)

                                   atgatgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCaaaaa                                                                                   <  2:87855/139‑1 (MQ=255)

                                       tggtggAACCACGGAAGAAAGGGTTCTGCGAGTTCAAGGCGTTTGACCGCAAACCAAATATCTAAGGGCGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTca                                                                               <  1:1016648/135‑1 (MQ=255)

                                       tgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTgc                                                                                                        >  2:396593/1‑114 (MQ=255)

                                       tgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTgc                                                                                                        <  1:396593/114‑1 (MQ=255)

                                                  cGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCt                                                                    <  2:380019/139‑1 (MQ=255)

                                                      tGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGc                                                                <  2:1015332/139‑1 (MQ=255)

                                                      tGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGc                                                                >  1:878170/1‑139 (MQ=255)

                                                       gAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTca                                                                               >  1:258429/1‑123 (MQ=255)

                                                       gAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTca                                                                               <  2:258429/123‑1 (MQ=255)

                                                       gAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGcc                                                               <  1:68734/139‑1 (MQ=255)

                                                       gAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGcc                                                               <  2:878170/139‑1 (MQ=255)

                                                               tCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTaa                                                       >  2:479365/1‑139 (MQ=255)

                                                               ccGGCGAGTCCATGCCGTTTCACGGCAAGCGAATGATCTATGGCGGCTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCGGTGCGCCGTGCCGTCAGCCCAAAAATCCACAAATGTTGCTGGGCGCAGCCTaa                                                       >  2:567670/2‑139 (MQ=255)

                                                                   cGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGaaa                                                   >  1:1009623/1‑139 (MQ=255)

                                                                        ccATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAAtcttc                                              >  1:102346/1‑139 (MQ=255)

                                                                                tttGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTa                                      >  2:974995/1‑139 (MQ=255)

                                                                                     cGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGcc                                 <  1:473315/139‑1 (MQ=255)

                                                                                        cAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGGCGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCaaaa                                                                                    <  1:526088/85‑1 (MQ=255)

                                                                                        cAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCaaaa                                                                                    >  2:526088/1‑85 (MQ=255)

                                                                                          aGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCt                            >  1:347522/1‑139 (MQ=255)

                                                                                                     tATGGCGGATTCGAGTCAATCATCGACGAATAAGTATCGTGACGGGCTGTGCGGCGTGCCGGCAGCCCAAAAATTCAGAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCCTGCTGTAGagccagc                 >  2:583075/1‑139 (MQ=255)

                                                                                                           ggATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGAc           >  2:226158/1‑139 (MQ=255)

                                                                                                            gATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGagc                     >  2:49209/1‑128 (MQ=255)

                                                                                                            gATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGagc                     <  1:49209/128‑1 (MQ=255)

                                                                                                             aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCg         >  1:65942/1‑139 (MQ=255)

                                                                                                              ttCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGt        >  2:317581/1‑139 (MQ=255)

                                                                                                                   gTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGc   <  1:982384/139‑1 (MQ=255)

                                                                                                                   gTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGc   >  1:474901/1‑139 (MQ=255)

                                                                                                                    tCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCa  <  2:65942/139‑1 (MQ=255)

CTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCA  >  NC_000913/476892‑477146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A