Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,969,987	G→A	A333V (GCG→GTG)	tap ←	methyl‑accepting protein IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,969,987	0	G	A	100.0%	149.3 / NA	47	A333V (GCG→GTG)	tap	methyl‑accepting protein IV
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (30/17); total (30/17)

GCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACT  >  NC_000913/1969861‑1970120

gCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCgg                                                                                                                           <  1:1019235/139‑1 (MQ=255)

    gAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCAtt                                                                                                                       >  1:813968/1‑139 (MQ=255)

     aaTATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCAttt                                                                                                                      >  2:1037242/1‑139 (MQ=255)

           ggTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGcc                                                                                                                >  2:567197/1‑139 (MQ=255)

            gTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCa                                                                                                               <  1:271327/139‑1 (MQ=255)

               tGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGtt                                                                                                            >  1:1044724/1‑139 (MQ=255)

                 gAAAGTAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTcc                                                                                                          >  2:106988/1‑139 (MQ=255)

                 gAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTcc                                                                                                          >  2:36894/1‑139 (MQ=255)

                                     cGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATc                                                                                      <  1:881582/139‑1 (MQ=255)

                                                tCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGcc                                                                           >  1:478410/1‑139 (MQ=255)

                                                tCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGcc                                                                           >  2:232557/1‑139 (MQ=255)

                                                           ttGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCg                                                                <  1:364911/139‑1 (MQ=255)

                                                                aGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTaa                                                           <  1:106988/139‑1 (MQ=255)

                                                                  cTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAAtt                                                         <  1:415414/139‑1 (MQ=255)

                                                                                        tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTTGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTaa                                                           <  2:484487/115‑1 (MQ=255)

                                                                                        tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTTGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTaa                                                           >  1:484487/1‑115 (MQ=255)

                                                                                        tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCg                                                                     <  1:720279/105‑1 (MQ=255)

                                                                                        tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTaa                                                           >  1:785969/1‑115 (MQ=255)

                                                                                        tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTaa                                                           <  2:785969/115‑1 (MQ=255)

                                                                                        tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTtg                                   >  2:580317/1‑139 (MQ=255)

                                                                                        tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTtg                                   >  2:581582/1‑139 (MQ=255)

                                                                                        tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTtg                                   >  1:171877/1‑139 (MQ=255)

                                                                                        tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTtg                                   >  2:432854/1‑139 (MQ=255)

                                                                                        tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTtg                                   >  1:527273/1‑139 (MQ=255)

                                                                                        tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTtg                                   >  2:172610/1‑139 (MQ=255)

                                                                                        tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTACCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCg                                                                     >  2:720279/1‑105 (MQ=255)

                                                                                                  ggTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTtgt                                  <  2:682402/130‑1 (MQ=255)

                                                                                                  ggTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTtgt                                  >  1:682402/1‑130 (MQ=255)

                                                                                                  ggTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGAt                         >  1:692458/1‑139 (MQ=255)

                                                                                                  ggTACTGACCTGAACACCGCCCGCCTGCACCGTTGTCGAGGCATTTTTTCTCAGTTACGCCGCCTGTCGTGCGTTATCGGCGCTTTGCCCTACCGTGGCGGTTAATTGTTCCATACTGGCGGCCGTTTGTGCCAGCGAt                         >  2:626368/1‑139 (MQ=255)

                                                                                                      cTGACCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATgccg                     >  2:637447/1‑139 (MQ=255)

                                                                                                         aCCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTggcgg                                          <  2:832493/115‑1 (MQ=255)

                                                                                                         aCCTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTggcgg                                          >  1:832493/1‑115 (MQ=255)

                                                                                                          ccTGAACACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCctg                 <  1:687506/139‑1 (MQ=255)

                                                                                                              aaCACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTGCGACGCCTGTCGTGCGTTATCGGCg                                                                                  >  2:746810/1‑70 (MQ=255)

                                                                                                              aaCACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCg                                                                                  <  1:746810/70‑1 (MQ=255)

                                                                                                              aaCACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCg                                                                >  2:432199/1‑88 (MQ=255)

                                                                                                              aaCACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCg                                                                <  1:432199/88‑1 (MQ=255)

                                                                                                              aaCACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGt             >  2:89306/1‑139 (MQ=255)

                                                                                                              aaCACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGt             >  2:974561/1‑139 (MQ=255)

                                                                                                              aaCACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGt             <  1:974561/139‑1 (MQ=255)

                                                                                                              aaCACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGt             >  1:856234/1‑139 (MQ=255)

                                                                                                              aaCACCGCCCGCCTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGt             >  1:544794/1‑139 (MQ=255)

                                                                                                                   cgcccgccTGCACCGTTGTCGGGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGt        >  2:636144/1‑139 (MQ=255)

                                                                                                                   cgcccgccTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGt        >  1:1014685/1‑139 (MQ=255)

                                                                                                                     cccgccTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTAc      <  2:1044724/139‑1 (MQ=255)

                                                                                                                         ccTGCACCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACt  <  2:171877/139‑1 (MQ=255)

GCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACT  >  NC_000913/1969861‑1970120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A