Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,366,953	G→A	G10R (GGA→AGA)	yhcF →	putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,366,953	0	G	A	100.0%	210.4 / NA	65	G10R (GGA→AGA)	yhcF	putative transcriptional regulator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/33); total (32/33)

AAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTC  >  NC_000913/3366821‑3367087

aaGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTg                                                                                                                                  <  2:99063/139‑1 (MQ=255)

                     aaTGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAgcgc                                                                                                             <  2:607073/139‑1 (MQ=255)

                         gCTCCAGCTTTTAAGGATGAAGGGGAACTAACGGACATGACAGATATTACGCGCAATCTTACGGTGCTGGTGGAGGCAAAATGAGCAATGTGAGATTACTGATTGGCAGAAGTGCCTTTTTTGCCATGGCAGCGCAAGc                                                                                                         <  2:748762/139‑1 (MQ=255)

                            ccAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGc                                                                                                      <  2:977196/139‑1 (MQ=255)

                             cAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCt                                                                                                     >  1:627526/1‑139 (MQ=255)

                              aGCTTTTAAGGATGAAGGGGAACTAACCGACATCACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTg                                                                                                    <  1:818195/139‑1 (MQ=255)

                               gCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGAGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   <  2:627526/139‑1 (MQ=255)

                                cTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                                  <  1:327317/139‑1 (MQ=255)

                                cTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                                  <  1:430805/139‑1 (MQ=255)

                                  tttAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGA‑TGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATaga                                                                                               <  2:349736/139‑1 (MQ=255)

                                          tGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCaa                                                                                        >  1:720421/1‑139 (MQ=255)

                                           gAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                       <  1:803692/139‑1 (MQ=255)

                                           gAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                       >  2:698053/1‑139 (MQ=255)

                                                      aaCCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAgg                                                                            <  2:275578/139‑1 (MQ=255)

                                                       aCCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  2:769337/109‑1 (MQ=255)

                                                       aCCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         >  1:769337/1‑109 (MQ=255)

                                                            cATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGa                                                                                                                                      >  2:807016/1‑75 (MQ=255)

                                                            cATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGa                                                                                                                                      <  1:807016/75‑1 (MQ=255)

                                                                      atTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGc                                                            >  2:279080/1‑139 (MQ=255)

                                                                       ttACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCa                                                                                                            <  2:401902/90‑1 (MQ=255)

                                                                       ttACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCa                                                                                                            >  1:401902/1‑90 (MQ=255)

                                                                       ttACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCa                                                           <  2:856806/139‑1 (MQ=255)

                                                                            ggCAATCTGAGGGATCTGGTGGAGGCAAAATGACTAATGGAAAATTACTGATTGTTAGAAGTGGCTTTTTTGCCATGTCAGCGCAAGTCGCTGATAGAGTATCAATTGACTTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      <  2:182376/139‑1 (MQ=255)

                                                                            ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATa                                                                                                 <  2:431014/96‑1 (MQ=255)

                                                                            ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATa                                                                                                 >  1:431014/1‑96 (MQ=255)

                                                                            ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      <  2:618573/139‑1 (MQ=255)

                                                                            ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      <  2:324203/139‑1 (MQ=255)

                                                                             ataagagacaggTCCTGGTGGAGTCAAAATGAACAATGTAAAATTACTGATTGCTAGAAGTGCCTTTTTTGCCATGTCAGCGCACGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGt                                                     <  2:694961/129‑1 (MQ=255)

                                                                              cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   >  1:809995/1‑92 (MQ=255)

                                                                              cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   <  2:809995/92‑1 (MQ=255)

                                                                              cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                    >  1:967432/1‑139 (MQ=255)

                                                                               aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTAc                                                   >  1:176575/1‑139 (MQ=255)

                                                                                aTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                    <  1:1044534/137‑1 (MQ=255)

                                                                                aTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                    >  2:1044534/1‑137 (MQ=255)

                                                                                aTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACt                                                  <  1:321860/139‑1 (MQ=255)

                                                                                 tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTc                                                 >  1:894165/1‑139 (MQ=255)

                                                                                     aCGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAAt                                             <  2:720421/139‑1 (MQ=255)

                                                                                         tCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTAt                                                                                           <  2:978678/89‑1 (MQ=255)

                                                                                         tCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTAt                                                                                           >  1:978678/1‑89 (MQ=255)

                                                                                           cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTaa                                                                              >  2:288787/1‑100 (MQ=255)

                                                                                           cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTaa                                                                              <  1:288787/100‑1 (MQ=255)

                                                                                           cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAg                                       >  1:912563/1‑139 (MQ=255)

                                                                                                   ggCAAAATGAACAATGTATAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACtctgtctattat                               >  1:694961/1‑128 (MQ=255)

                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGcc                                                                                                        <  1:350119/66‑1 (MQ=255)

                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGcc                                                                                                        >  2:350119/1‑66 (MQ=255)

                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCt                                                                                                     <  2:6288/69‑1 (MQ=255)

                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCt                                                                                                     >  1:6288/1‑69 (MQ=255)

                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   <  2:180796/71‑1 (MQ=255)

                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   >  1:180796/1‑71 (MQ=255)

                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                               >  1:111222/1‑139 (MQ=255)

                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                               >  1:1033333/1‑139 (MQ=255)

                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGAGACTCTGTAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                               >  1:621694/1‑139 (MQ=255)

                                                                                                     cAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGa                             <  2:912563/139‑1 (MQ=255)

                                                                                                     cAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGa                             <  2:17137/139‑1 (MQ=255)

                                                                                                     cAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGa                             >  2:215575/1‑139 (MQ=255)

                                                                                                             acgaTGTAAAAGTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         >  2:611717/4‑55 (MQ=255)

                                                                                                             aCAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  1:611717/55‑1 (MQ=255)

                                                                                                                   tAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCgg               <  1:184034/139‑1 (MQ=255)

                                                                                                                           cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGGGCAAGCCGCGGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCAtt       >  2:745055/1‑139 (MQ=255)

                                                                                                                           cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTTACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGGGGAGTTGTTAACTTCGGTAGTCAtt       >  2:60611/1‑139 (MQ=255)

                                                                                                                           cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCAtt       >  1:340221/1‑139 (MQ=255)

                                                                                                                           cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCAtt       >  2:896047/1‑139 (MQ=255)

                                                                                                                             gATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCa     >  1:696574/1‑139 (MQ=255)

                                                                                                                                tGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTc  >  2:577042/1‑139 (MQ=255)

                                                                                                                                tGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTc  >  2:931337/1‑139 (MQ=255)

AAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTC  >  NC_000913/3366821‑3367087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A