Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,441,207 |
C→T |
G50G (GGC→GGT) |
ydbJ → |
DUF333 family putative lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,441,207 | 0 | C | T | 100.0%
| 96.4
/ NA
| 32 | G50G (GGC→GGT) | ydbJ | DUF333 family putative lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/16); total (16/16) |
TCGGCGTGTAGTAGTGAACCTGTTCAGCAGGCGACTGCCGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGC > NC_000913/1441100‑1441338
|
tCGGCGTGTAGTAGTGAACCTGTTCAGCAGGCGACTGCCGCGCACATAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGTAATGATCGGTGGTTCGCTTTCTGTTGCCCGTc < 1:934418/130‑1 (MQ=255)
tCGGCGTGTAGTAGTGAACCTGTTCAGCAGGCGACTGCCGCGCACATAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGTAATGATCGGTGGTTCGCTTTCTGTTGCCCGTc > 2:934418/1‑130 (MQ=255)
ggCGTGTAGTAGTGAACCTGTTCAGCAGGCGACTGCCGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGt > 2:922197/1‑139 (MQ=255)
tGAACCTGTTCAGCAGGCGACTGCCGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCAGTCAACTGGATGGTACGGCGATTggg < 1:777197/139‑1 (MQ=255)
ccTGTTCAGCAGGCGACTGCCGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGAtgt > 2:1263543/1‑139 (MQ=255)
ccTGTTCAGCAGGCGACTGCCGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGAtgt < 1:922197/139‑1 (MQ=255)
tCAGCAGGCGACTGCCGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCa < 2:736473/139‑1 (MQ=255)
tCAGCAGGCGACTGCCGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCa > 2:1101139/1‑139 (MQ=255)
cACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCt > 1:733828/1‑139 (MQ=255)
gTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTggg > 2:1006364/1‑88 (MQ=255)
gTCGATGTCAAGTAGTGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTggg < 1:1006364/88‑1 (MQ=255)
tGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAg < 1:1263543/139‑1 (MQ=255)
cAGTAGTGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATgg > 1:682501/1‑66 (MQ=255)
cAGTAGTGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATgg < 2:682501/66‑1 (MQ=255)
tagtGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGc < 1:1101139/139‑1 (MQ=255)
tGGAGAAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGc > 1:836364/1‑139 (MQ=255)
gagaAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACt < 1:711398/52‑1 (MQ=255)
gagaAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACt > 2:711398/1‑52 (MQ=255)
gagaAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACgg > 1:593464/1‑63 (MQ=255)
gagaAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACgg < 2:593464/63‑1 (MQ=255)
gagaAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCa < 1:904774/87‑1 (MQ=255)
gagaAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACg < 2:975482/97‑1 (MQ=255)
gagaAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACg > 1:975482/1‑97 (MQ=255)
gagaAGCAAATTGCGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCa > 2:904774/1‑87 (MQ=255)
gaAGCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTAtt > 1:891954/1‑139 (MQ=255)
gCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGcc < 2:687414/39‑1 (MQ=255)
gCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGcc > 1:687414/1‑39 (MQ=255)
gCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGt < 2:291748/42‑1 (MQ=255)
gCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGt > 1:291748/1‑42 (MQ=255)
gCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCa < 2:1043086/130‑1 (MQ=255)
gCAAATTGTGCAATGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCa > 1:1043086/1‑130 (MQ=255)
tGATCGGTGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGACAGCTATTAATTCATTAAATCCGc < 1:248606/139‑1 (MQ=255)
|
TCGGCGTGTAGTAGTGAACCTGTTCAGCAGGCGACTGCCGCGCACGTAGCGCCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTGCCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGTAGCGAACAGTCACTTGCCGCCGGGAGCTGTGGCAGCTATTAATTCATTAAATCCGC > NC_000913/1441100‑1441338
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
