Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,764,018 |
C→T |
*123* (TAG→TAA) |
sufA ← |
Fe‑S cluster assembly protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,764,018 | 0 | C | T | 100.0%
| 83.6
/ NA
| 27 | *123* (TAG→TAA) | sufA | Fe‑S cluster assembly protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/12); total (15/12) |
CTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCA > NC_000913/1763887‑1764145
|
ctctTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATA‑cccc > 1:582606/1‑139 (MQ=255)
tGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCgccacagcc > 1:1027219/1‑139 (MQ=255)
tCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACattcattc > 1:1213112/1‑139 (MQ=255)
aaCTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTa < 2:1027219/139‑1 (MQ=255)
tGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAggg > 1:37800/1‑139 (MQ=255)
ttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATc < 2:37800/139‑1 (MQ=255)
ttCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTa < 2:582606/139‑1 (MQ=255)
cAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAg < 2:1213112/139‑1 (MQ=255)
aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCCAAAGCTTTCGCCACAGCCACATTCATTCTg > 2:1302187/1‑103 (MQ=255)
aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCCAAAGCTTTCGCCACAGCCACATTCATTCTg < 1:1302187/103‑1 (MQ=255)
aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTg < 1:590314/102‑1 (MQ=255)
aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTg > 2:590314/1‑102 (MQ=255)
cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTAAGAGACATAGTACCGCTTATA‑CCCCAAAGATTTCGCCACAGCCAAATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATATGATTAAGTcc > 2:1013649/1‑139 (MQ=255)
cgccgGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAatat < 1:41050/122‑1 (MQ=255)
cgccgGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAatat > 2:41050/1‑122 (MQ=255)
gACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTc > 1:75959/1‑139 (MQ=255)
attGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTg > 1:610459/2‑70 (MQ=255)
attGCTTCAGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTg < 2:610459/69‑1 (MQ=255)
aGTATTACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACg > 2:471678/1‑139 (MQ=255)
ttACGAGACATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCAt < 2:75959/139‑1 (MQ=255)
gaCATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCt < 2:741486/51‑1 (MQ=255)
gaCATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCt > 1:741486/1‑51 (MQ=255)
aCATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAatat < 2:21596/78‑1 (MQ=255)
aCATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAatat > 1:21596/1‑78 (MQ=255)
cATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGc > 1:168587/1‑129 (MQ=255)
cATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGc < 2:168587/129‑1 (MQ=255)
cATAGTACCGCTTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCa > 2:1345602/1‑139 (MQ=255)
|
CTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATA‑CCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCA > NC_000913/1763887‑1764145
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
