Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 765 53.2 2691310 93.4% 2513683 103.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,792,267 C→T *155* (TAG→TAA)  nlpC ← putative C40 clan peptidase lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,792,2670CT100.0% 68.9 / NA 23*155* (TAG→TAA) nlpCputative C40 clan peptidase lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/12);  total (11/12)

ATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTA  >  NC_000913/1792154‑1792372
                                                                                                                 |                                                                                                         
aTTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAAt                                                                                  >  2:810636/1‑139 (MQ=255)
                             ttACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTAt                                                             <  2:243981/131‑1 (MQ=255)
                             ttACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTAt                                                             >  1:243981/1‑131 (MQ=255)
                                  gAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACg                                                <  1:810636/139‑1 (MQ=255)
                                                            tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTa                             <  1:147702/132‑1 (MQ=255)
                                                            tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTa                             >  2:147702/1‑132 (MQ=255)
                                                            tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTAGTAGAGGCGt                      >  1:1202093/1‑139 (MQ=255)
                                                               tCAGGAGTAAATGGACGGCGAGGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGATATTATCATGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGa                   <  2:292850/139‑1 (MQ=255)
                                                                   gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTg                                                                           >  1:770184/1‑79 (MQ=255)
                                                                   gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATaaa               >  1:210073/1‑139 (MQ=255)
                                                                   gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCATGCCAGAAATTTTTCTg                                                                           <  2:770184/79‑1 (MQ=255)
                                                                      tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTAt                                                             >  2:570958/1‑90 (MQ=255)
                                                                      tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTAt                                                             <  1:570958/90‑1 (MQ=255)
                                                                               ggCGATGTAAAATCGCCGTCCATTTTTCGACATATTTAATTCGTCTCGGCTGCCAAAAATTTTTCTGCCAAAAGAAATTATCAAGTTAGGAACGCAACACTTCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGt   <  1:215170/139‑1 (MQ=255)
                                                                                gCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTAGTAGAGGCGTGGATAAATTGGTTGTTGGTa  <  2:1202093/139‑1 (MQ=255)
                                                                                           tcGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgttgt       >  1:682978/1‑123 (MQ=255)
                                                                                           tcGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgttgt       <  2:682978/123‑1 (MQ=255)
                                                                                                gTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCt                                                                            <  2:233151/49‑1 (MQ=255)
                                                                                                gTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCt                                                                            >  1:233151/1‑49 (MQ=255)
                                                                                                  ccATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGAt                  >  2:417577/1‑105 (MQ=255)
                                                                                                  ccATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGAt                  <  1:417577/105‑1 (MQ=255)
                                                                                                             cATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGaa                                                  <  1:1224114/62‑1 (MQ=255)
                                                                                                             cATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGaa                                                  >  2:1224114/1‑62 (MQ=255)
                                                                                                                 |                                                                                                         
ATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTA  >  NC_000913/1792154‑1792372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A