Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 765 53.2 2691310 93.4% 2513683 103.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,467,233 A→G Y230C (TAC→TGC)  intS → CPS‑53 (KpLE1) prophage, putative prophage CPS‑53 integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,467,2330AG100.0% 190.5 / NA 52Y230C (TAC→TGC) intSCPS‑53 (KpLE1) prophage, putative prophage CPS‑53 integrase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/24);  total (28/24)

GGGATACCGCAAGAAGAACTTCCCGTTTCTTCCTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTACACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCA  >  NC_000913/2467114‑2467362
                                                                                                                       |                                                                                                                                 
gggATACCGCAAGAAGAACTTCCCGTTTCTTCCTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGaaa                                                                                                                <  1:595919/139‑1 (MQ=255)
     aCCGCAAGAAGAACTTCCCGTTTCTTCCTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCt                                                                                                           >  2:317326/1‑139 (MQ=255)
         cAAGAAGAACTTCCCGTTTCTTCCTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGt                                                                                                       >  1:1146261/1‑139 (MQ=255)
           agaagaACTTCCCGTTTCTTCCTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTc                                                                                                     >  2:871150/1‑139 (MQ=255)
           agaagaACTTCCCGTTTCTTCCTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTc                                                                                                     >  1:1328644/1‑139 (MQ=255)
             aagaaCTTCCCGTTTCTTCCTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCa                                                                                                   <  2:10848/139‑1 (MQ=255)
               gaaCTTCCCGTTTCTTCCTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATg                                                                                                 <  2:1328644/139‑1 (MQ=255)
                aaCTTCCCGTTTCTTCCTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATg                                                                                                 <  1:40265/138‑1 (MQ=255)
                aaCTTCCCGTTTCTTCCTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATg                                                                                                 >  2:40265/1‑138 (MQ=255)
                aaCTTCCCGTTTCTTCCTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGc                                                                                                >  2:1228558/1‑139 (MQ=255)
                          ttcttcCTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTgca                                                                                                                                 >  1:917112/1‑96 (MQ=255)
                               ccTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCg                                                                                 >  2:146072/1‑139 (MQ=255)
                                   cAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGAttt                                                                             >  1:603290/1‑139 (MQ=255)
                                        cAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCt                                                                                                           <  1:164355/104‑1 (MQ=255)
                                        cAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCt                                                                                                           >  2:164355/1‑104 (MQ=255)
                                           aTCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACC‑AAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAg                                                                    >  2:710663/1‑139 (MQ=255)
                                             cccGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACC‑AAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGa                                                                  <  1:710663/139‑1 (MQ=255)
                                               cGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGAt                                                                 >  2:175266/1‑139 (MQ=255)
                                                tgCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCAATTTTGAAAATAGGAtt                                                                >  1:828247/2‑139 (MQ=255)
                                                    ttCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCa                                                            <  2:603290/139‑1 (MQ=255)
                                                     tCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCAc                                                           >  1:22201/1‑139 (MQ=255)
                                                     tCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCAc                                                           >  1:370851/1‑139 (MQ=255)
                                                            aGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATg                                                                                                 >  2:111311/1‑94 (MQ=255)
                                                            aGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATg                                                                                                 <  1:111311/94‑1 (MQ=255)
                                                              cACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGtt                                                                                                      >  2:1153659/1‑87 (MQ=255)
                                                              cACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGtt                                                                                                      <  1:1153659/87‑1 (MQ=255)
                                                              cACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGc                                                  <  2:377456/139‑1 (MQ=255)
                                                               aCTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGcc                                                 <  1:317326/139‑1 (MQ=255)
                                                                cTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCa                                                <  1:871150/139‑1 (MQ=255)
                                                                 tGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAg                                               >  1:1237832/1‑139 (MQ=255)
                                                                   gCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCt                                                                                                                          <  1:160746/62‑1 (MQ=255)
                                                                   gCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCt                                                                                                                          >  2:160746/1‑62 (MQ=255)
                                                                                           tcgCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCAtg                     <  2:22201/139‑1 (MQ=255)
                                                                                           tcgCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCAtg                     >  2:669509/1‑139 (MQ=255)
                                                                                           tcgCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCAtg                     <  2:1237832/139‑1 (MQ=255)
                                                                                            cgCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGtt                                                                                                      >  2:1032549/1‑57 (MQ=255)
                                                                                            cgCTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGtt                                                                                                      <  1:1032549/57‑1 (MQ=255)
                                                                                              cTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCa                                                                                                   >  2:208581/1‑58 (MQ=255)
                                                                                              cTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCa                                                                                                   <  1:546166/58‑1 (MQ=255)
                                                                                              cTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCa                                                                                                   <  1:208581/58‑1 (MQ=255)
                                                                                              cTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCa                                                                                                   >  2:546166/1‑58 (MQ=255)
                                                                                              cTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGaaaa                                                                       >  2:200227/1‑86 (MQ=255)
                                                                                              cTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGaaaa                                                                       <  1:200227/86‑1 (MQ=255)
                                                                                              cTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCAtg                     >  1:427074/1‑136 (MQ=255)
                                                                                              cTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCAtg                     <  2:427074/136‑1 (MQ=255)
                                                                                              cTCATTGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTgg                  >  1:353639/1‑139 (MQ=255)
                                                                                                  ttGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTcc              <  2:828247/139‑1 (MQ=255)
                                                                                                   tGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGa                                                                  >  1:1049998/1‑86 (MQ=255)
                                                                                                   tGCGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGa                                                                  <  2:1049998/86‑1 (MQ=255)
                                                                                                     cGACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTAt           >  1:218039/1‑139 (MQ=255)
                                                                                                      gACCAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATg          <  1:175266/139‑1 (MQ=255)
                                                                                                        ccAAAGTTTTACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTc        >  2:1336713/1‑139 (MQ=255)
                                                                                                              ttttACGCTGCACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCa  <  2:218039/139‑1 (MQ=255)
                                                                                                                       |                                                                                                                                 
GGGATACCGCAAGAAGAACTTCCCGTTTCTTCCTGCAGACCAGATCCCGGCATTCAACAAAGCACTGGCAACATTTTCAGGAAGTATCGTATCGCTCATTGCGACCAAAGTTTTACGCTACACAGCCCTAAGAACGAAAGAGCTTCGTTCCATGCTATGGAAGAACGTCGATTTTGAAAATAGGATTATCACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCA  >  NC_000913/2467114‑2467362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A