Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,350,184 |
(C)7→8 |
coding (276/654 nt) |
elbB ← |
isoprenoid biosynthesis protein with amidotransferase‑like domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,350,177 | 1 | . | C | 100.0%
| 143.7
/ NA
| 41 | F95V (TTT→GTT) | elbB | isoprenoid biosynthesis protein with amidotransferase‑like domain |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (20/21); total (20/21) |
CCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAA‑‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATG > NC_000913/3350045‑3350309
|
ccGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAA‑‑cccccc < 2:1195900/139‑1 (MQ=255)
gCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAA‑‑ccccccccggc > 2:1105528/1‑135 (MQ=255)
tACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACaa > 2:1080845/1‑139 (MQ=255)
atgacaCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCGCGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCGTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAAc < 1:496275/134‑1 (MQ=255)
aTAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAAc > 2:627496/1‑139 (MQ=255)
aaaaCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGc > 2:1226525/1‑139 (MQ=255)
gCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAAc > 2:667684/1‑129 (MQ=255)
gCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAAc < 1:667684/129‑1 (MQ=255)
cGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt < 2:1083297/139‑1 (MQ=255)
cGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt < 1:627496/139‑1 (MQ=255)
tGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGcc < 1:1080845/139‑1 (MQ=255)
cTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAA‑‑ccccccccggc < 2:1285307/96‑5 (MQ=255)
cTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAA‑‑ccccccccggc > 1:1285307/1‑92 (MQ=255)
cgcCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACCCCCCCCCGGCACAATCAACGCATCCAGTTCAg > 1:646081/1‑109 (MQ=255)
cgcCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACCCCCCCCCGGCACAATCAACGCATCCAGTTCAg < 2:646081/109‑1 (MQ=255)
cgcCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTc > 2:436936/1‑139 (MQ=255)
cgcCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTc > 2:591168/1‑139 (MQ=255)
gcCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt < 1:696546/113‑1 (MQ=255)
gcCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt > 2:696546/1‑113 (MQ=255)
tttAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACcg < 1:1136625/139‑1 (MQ=255)
tCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt > 1:245372/1‑104 (MQ=255)
tCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt < 2:245372/104‑1 (MQ=255)
cGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTAtt > 1:212224/1‑139 (MQ=255)
aCGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGcc > 1:213201/1‑139 (MQ=255)
gTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCt < 1:1105528/139‑1 (MQ=255)
tGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGAGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCtt > 2:1240842/1‑139 (MQ=255)
gCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt > 1:670419/1‑90 (MQ=255)
gCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCAt < 2:670419/90‑1 (MQ=255)
aTTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCaa > 1:894301/1‑139 (MQ=255)
tCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGAGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATc < 1:1240842/139‑1 (MQ=255)
aCCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGcac > 1:236831/1‑139 (MQ=255)
cAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCa > 1:692017/1‑49 (MQ=255)
cAAAATTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCa < 2:692017/49‑1 (MQ=255)
aTTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGtt > 1:412972/1‑134 (MQ=255)
aTTGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGtt < 2:412972/134‑1 (MQ=255)
ttGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTc > 2:525360/1‑139 (MQ=255)
ttGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTc < 2:894301/139‑1 (MQ=255)
tGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAg < 1:1238812/119‑1 (MQ=255)
tGCTTAAATTCTTCGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAg > 2:1238812/1‑119 (MQ=255)
tcttcGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGc < 2:212224/139‑1 (MQ=255)
cttcGCCGCGCCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGcc < 1:525360/139‑1 (MQ=255)
gcgcCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTaaa < 1:1226525/139‑1 (MQ=255)
cgcCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAg > 1:164315/1‑40 (MQ=37)
cgcCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAg < 2:164315/40‑1 (MQ=37)
gcCAAAC‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATg < 2:236831/139‑1 (MQ=255)
|
CCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAA‑‑CCCCCCCGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATG > NC_000913/3350045‑3350309
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
