Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	992,825	A→G	Y735C (TAC→TGC)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	992,825	0	A	G	100.0%	176.7 / NA	49	Y735C (TAC→TGC)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/31); total (18/31)

TGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAA  >  NC_000913/992691‑992960

tGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTgcgac                                                                                                                                     <  1:861110/139‑1 (MQ=255)

     aaaCGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACaa                                                                                                                                >  2:1364689/1‑139 (MQ=255)

      aaCGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAg                                                                                                                               <  2:253751/139‑1 (MQ=255)

       aCGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGt                                                                                                                              <  2:1014176/139‑1 (MQ=255)

       aCGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCCGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGt                                                                                                                              >  1:1321777/1‑139 (MQ=255)

           aTCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCa                                                                                                                          >  1:1048404/1‑139 (MQ=255)

             cTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTACCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGcgacga                                                                                                                                   >  1:1063715/1‑128 (MQ=255)

             cTGGCTGATGGGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGcgacga                                                                                                                                   <  2:1063715/128‑1 (MQ=255)

                 cTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                                    <  2:676358/139‑1 (MQ=255)

                 cTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                                    <  2:1321777/139‑1 (MQ=255)

                 cTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                                    <  1:426342/139‑1 (MQ=255)

                     tgtgCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGt                                                                                                                              >  2:946941/1‑125 (MQ=255)

                     tgtgCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGt                                                                                                                              <  1:946941/125‑1 (MQ=255)

                      gtgCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTc                                                                                                               <  1:106237/139‑1 (MQ=255)

                          tGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGGCGACAAGTGGCATCAGAACGGTCTGGt                                                                                                           <  1:1618009/139‑1 (MQ=255)

                          tGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCAt                                                                                                                         >  1:1256842/1‑125 (MQ=255)

                          tGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCAt                                                                                                                         <  2:1256842/125‑1 (MQ=255)

                          tGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGt                                                                                                                <  1:633194/134‑1 (MQ=255)

                          tGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGt                                                                                                                >  2:633194/1‑134 (MQ=255)

                            gTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGa                                                                                                         <  2:1019712/139‑1 (MQ=255)

                            gTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGa                                                                                                         <  1:99703/139‑1 (MQ=255)

                              gAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                                    >  1:154426/1‑126 (MQ=255)

                              gAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                                    <  2:154426/126‑1 (MQ=255)

                                 caagcaGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGAt                                                                                                    >  2:119851/1‑139 (MQ=255)

                                   agcaGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATaa                                                                                                  <  1:539775/139‑1 (MQ=255)

                                                  cAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                                    >  1:959215/1‑106 (MQ=255)

                                                  cAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                                    <  2:959215/106‑1 (MQ=255)

                                                          atGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGt                                                                                                                              >  2:678461/1‑88 (MQ=255)

                                                          atGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGt                                                                                                                              <  1:678461/88‑1 (MQ=255)

                                                                 aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                                    <  1:1130667/91‑1 (MQ=255)

                                                                 aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                                    >  2:1130667/1‑91 (MQ=255)

                                                                 aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATaa                                                                                                  >  2:823411/1‑109 (MQ=255)

                                                                 aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATaa                                                                                                  <  1:823411/109‑1 (MQ=255)

                                                                      cTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGa                                                               <  1:1364689/139‑1 (MQ=255)

                                                                       tGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGaa                                                              <  1:749311/139‑1 (MQ=255)

                                                                                         cggTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTgcg                                            <  1:554576/139‑1 (MQ=255)

                                                                                           gTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAg                                                                                                                      <  1:627513/63‑1 (MQ=255)

                                                                                           gTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAg                                                                                                                      >  2:627513/1‑63 (MQ=255)

                                                                                             tGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGc                                        <  1:119851/139‑1 (MQ=255)

                                                                                             tGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATAAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGc                                        <  2:1048404/139‑1 (MQ=255)

                                                                                              gCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGcc                                       <  1:910399/139‑1 (MQ=255)

                                                                                                      gCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGAAGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTgcag                               >  2:1143076/1‑139 (MQ=255)

                                                                                                       cTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                                    <  1:857876/53‑1 (MQ=255)

                                                                                                       cTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                                    >  2:857876/1‑53 (MQ=255)

                                                                                                           cTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCg                          <  1:1149565/139‑1 (MQ=255)

                                                                                                                         cTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                                    >  1:124766/1‑35 (MQ=255)

                                                                                                                         cTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                                    <  2:124766/35‑1 (MQ=255)

                                                                                                                         cTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCcggc                                                                    <  2:1644733/83‑1 (MQ=255)

                                                                                                                         cTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCcggc                                                                    >  1:1644733/1‑83 (MQ=255)

                                                                                                                                   aGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGaa  <  1:728400/139‑1 (MQ=255)

TGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCATGAGCAACCCGAA  >  NC_000913/992691‑992960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A