Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,212,703	C→T	135 (TAG→TAA)	ycgX ←	DUF1398 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,212,703	0	C	T	100.0%	133.2 / NA	42	135 (TAG→TAA)	ycgX	DUF1398 family protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/20); total (22/20)

TAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTG  >  NC_000913/1212569‑1212830

tAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGa                                                                                                                             >  1:773126/1‑139 (MQ=255)

       gTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCAACCATAGAGACAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATgg                                                                                                                      >  1:1106300/1‑139 (MQ=255)

           ttatttatGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTtta                                                                                                                  <  2:737471/139‑1 (MQ=255)

            tatttatGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTttat                                                                                                                 >  2:339422/1‑139 (MQ=255)

            tatttatGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTttat                                                                                                                 >  2:826722/1‑139 (MQ=255)

            tatttatGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGAATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTttat                                                                                                                 >  2:907285/1‑139 (MQ=255)

                           aTAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCtt                                                                                                  <  1:711593/139‑1 (MQ=255)

                                  gACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAatata                                                                                           <  1:826722/139‑1 (MQ=255)

                                       tATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACAt                                                                                                     <  2:328007/124‑1 (MQ=255)

                                       tATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACAt                                                                                                     >  1:328007/1‑124 (MQ=255)

                                             tcatcCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCtt                                                                                                  >  1:1778658/1‑121 (MQ=255)

                                             tcatcCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCtt                                                                                                  <  2:1778658/121‑1 (MQ=255)

                                                 ccATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTAc                                                                                                       >  2:1160309/1‑112 (MQ=255)

                                                 ccATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTCAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTAc                                                                                                       <  1:1160309/112‑1 (MQ=255)

                                                           aGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCgtag                                                                  >  2:578559/1‑139 (MQ=255)

                                                            gTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCa                                                                           >  1:1145393/1‑129 (MQ=255)

                                                            gTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCa                                                                           <  2:1145393/129‑1 (MQ=255)

                                                               tctGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGc                                                                                    >  1:204272/1‑117 (MQ=255)

                                                               tctGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGc                                                                                    <  2:204272/117‑1 (MQ=255)

                                                                    aCTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGt                                                         <  2:773126/139‑1 (MQ=255)

                                                                        ttATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGGGTAGTAACTGCGTTTGt                                                     <  1:339422/139‑1 (MQ=255)

                                                                                         aTGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGt                                                                                  <  2:608537/93‑1 (MQ=255)

                                                                                         aTGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGt                                                                                  >  1:608537/1‑93 (MQ=255)

                                                                                              ttACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTtta                                                                                                                  >  2:999065/1‑56 (MQ=255)

                                                                                              ttACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTtta                                                                                                                  <  1:999065/56‑1 (MQ=255)

                                                                                                aCTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTAc                                                                                                       <  1:393179/65‑1 (MQ=255)

                                                                                                aCTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTAc                                                                                                       >  2:393179/1‑65 (MQ=255)

                                                                                                         tGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCg                                                                     >  2:1297011/1‑90 (MQ=255)

                                                                                                         tGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCg                                                                     <  1:1297011/90‑1 (MQ=255)

                                                                                                         tGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTaa                                                               <  1:373446/96‑1 (MQ=255)

                                                                                                         tGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTaa                                                               >  2:373446/1‑96 (MQ=255)

                                                                                                           aaGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGt                                                     >  2:120207/1‑104 (MQ=255)

                                                                                                           aaGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGt                                                     <  1:120207/104‑1 (MQ=255)

                                                                                                           aaGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTaa                                            >  1:407284/1‑113 (MQ=255)

                                                                                                           aaGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTaa                                            <  2:407284/113‑1 (MQ=255)

                                                                                                                        gTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAgtg     <  1:578559/139‑1 (MQ=255)

                                                                                                                           tCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCtt                                                                                                  >  1:1658629/1‑43 (MQ=255)

                                                                                                                           tCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCtt                                                                                                  <  2:1658629/43‑1 (MQ=255)

                                                                                                                           tCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGc          <  1:285519/131‑1 (MQ=255)

                                                                                                                           tCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGc          >  2:285519/1‑131 (MQ=255)

                                                                                                                           tCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTg  >  1:1907/1‑139 (MQ=255)

                                                                                                                           tCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTg  >  2:441695/1‑139 (MQ=255)

TAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTG  >  NC_000913/1212569‑1212830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A