Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,789,804	G→A	64 (TAG→TAA)	ydiE →	hemin uptake protein HemP homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,789,804	0	G	A	100.0%	202.0 / NA	64	64 (TAG→TAA)	ydiE	hemin uptake protein HemP homolog
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/32); total (32/32)

CTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAGCCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCT  >  NC_000913/1789671‑1789935

cTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCttt                                                                                                                                >  1:669655/1‑139 (MQ=255)

cTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCttt                                                                                                                                >  1:886111/1‑139 (MQ=255)

           ccTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGagcag                                                                                                                     >  1:762665/1‑139 (MQ=255)

             tATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCt                                                                                                                   <  2:868800/139‑1 (MQ=255)

             tATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCt                                                                                                                   <  2:378775/139‑1 (MQ=255)

                      aaTTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAg                                                                                                          <  1:187735/139‑1 (MQ=255)

                      aaTTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAg                                                                                                          <  2:843188/139‑1 (MQ=255)

                                   acacTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAg                                                                                             >  1:1217006/1‑139 (MQ=255)

                                           aGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTgg                                                                                     <  1:740775/139‑1 (MQ=255)

                                             gcccGGATGTCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCATTTACCCCAGTCAGGTGgac                                                                                   <  1:1057949/137‑1 (MQ=255)

                                              tCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTc                                                                                                             >  2:554564/1‑112 (MQ=255)

                                              tCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTc                                                                                                             <  1:554564/112‑1 (MQ=255)

                                                 ggATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACt                                                                               >  1:947318/1‑139 (MQ=255)

                                                  gATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTg                                                                              <  2:669655/139‑1 (MQ=255)

                                                             gATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGtcatc                                                                   >  1:674295/1‑139 (MQ=255)

                                                              aTTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGtcatca                                                                  >  1:45665/1‑139 (MQ=255)

                                                                tATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCATGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCatcatc                                                                >  2:1177153/1‑139 (MQ=255)

                                                                 aTCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGagcag                                                                                                                     >  2:369198/1‑85 (MQ=255)

                                                                 aTCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGagcag                                                                                                                     <  1:369198/85‑1 (MQ=255)

                                                                    gATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTg                                                            <  2:45665/139‑1 (MQ=255)

                                                                      tCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTc                                                          >  2:140151/1‑139 (MQ=255)

                                                                        aTGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGagcagc                                                                                                                    <  1:639983/79‑1 (MQ=255)

                                                                        aTGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGagcagc                                                                                                                    >  2:639983/1‑79 (MQ=255)

                                                                        aTGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTgg                                                                                     >  2:714307/1‑110 (MQ=255)

                                                                        aTGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTgg                                                                                     <  1:714307/110‑1 (MQ=255)

                                                                        aTGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                           >  1:1350502/1‑120 (MQ=255)

                                                                        aTGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                           <  2:1350502/120‑1 (MQ=255)

                                                                        aTGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGc                                                        >  1:309727/1‑139 (MQ=255)

                                                                                cAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGa                                                >  2:469558/1‑139 (MQ=255)

                                                                                      tatCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGc                                          <  1:535587/139‑1 (MQ=255)

                                                                                       atCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCATGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCa                                         <  1:1177153/139‑1 (MQ=255)

                                                                                       atCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCa                                         <  1:1716979/139‑1 (MQ=255)

                                                                                         cTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAAc                                       >  2:1729753/1‑139 (MQ=255)

                                                                                           gCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGc                                     <  1:36466/139‑1 (MQ=255)

                                                                                           gCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGc                                     <  2:762665/139‑1 (MQ=255)

                                                                                           gCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGc                                     <  1:1115923/139‑1 (MQ=255)

                                                                                             tCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCa                                         <  1:628679/133‑1 (MQ=255)

                                                                                             tCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCa                                         >  2:628679/1‑133 (MQ=255)

                                                                                             tCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCt                                   >  2:863073/1‑139 (MQ=255)

                                                                                              ccGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGtc                                                                      <  2:1196080/103‑1 (MQ=255)

                                                                                              ccGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGtc                                                                      >  1:1196080/1‑103 (MQ=255)

                                                                                               cGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGga                                                                                    >  2:932209/1‑88 (MQ=255)

                                                                                               cGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGga                                                                                    <  1:932209/88‑1 (MQ=255)

                                                                                                 tAAAACTCAGGCTGGCAAGCTGCTGTTGACCACGTAAGCTTTAACTCGAGCAGCTGACTTCCCGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATg                               <  1:239993/139‑1 (MQ=255)

                                                                                                 tAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCg                                                                                                       >  2:962081/1‑67 (MQ=255)

                                                                                                 tAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCg                                                                                                       <  1:962081/67‑1 (MQ=255)

                                                                                                 tAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATg                               >  2:1061952/1‑139 (MQ=255)

                                                                                                     aCTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAgg                           <  1:1279674/139‑1 (MQ=255)

                                                                                                      cTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATa                                                                         >  2:818063/1‑92 (MQ=255)

                                                                                                      cTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATa                                                                         <  1:818063/92‑1 (MQ=255)

                                                                                                         aGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGt                       >  1:1547212/1‑139 (MQ=255)

                                                                                                           gCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTc                                                                                                             >  1:276032/1‑51 (MQ=255)

                                                                                                           gCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTc                                                                                                             <  2:276032/51‑1 (MQ=255)

                                                                                                                cAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                           <  1:152495/80‑1 (MQ=255)

                                                                                                                cAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                           >  2:152495/1‑80 (MQ=255)

                                                                                                                cAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCAtct                                                               >  2:286982/1‑92 (MQ=255)

                                                                                                                cAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCAtct                                                               <  1:286982/92‑1 (MQ=255)

                                                                                                                       ctgTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCata         <  1:1729753/139‑1 (MQ=255)

                                                                                                                             aCCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCAccc   >  2:120816/1‑139 (MQ=255)

                                                                                                                              ccAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCt  >  2:150009/1‑139 (MQ=255)

                                                                                                                               cAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCa      <  1:595859/134‑1 (MQ=255)

                                                                                                                               cAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCa      >  2:595859/1‑134 (MQ=255)

                                                                                                                                 aGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGgacgac                                                                                <  1:1331007/58‑1 (MQ=255)

                                                                                                                                 aGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGgacgac                                                                                >  2:1331007/1‑58 (MQ=255)

CTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAGCCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCT  >  NC_000913/1789671‑1789935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A