Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	500,360	A→G	E79G (GAA→GGA)	gsk →	inosine/guanosine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	500,360	0	A	G	100.0%	227.7 / NA	62	E79G (GAA→GGA)	gsk	inosine/guanosine kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (32/30); total (32/30)

GTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGAACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTCGCGGACGACCGTTCGGTGCTGCTGGGCGTCATGTGCAGCAATATTG  >  NC_000913/500233‑500485

gTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGa                                                                                                                    >  1:598441/1‑139 (MQ=255)

       tCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAAc                                                                                                                       <  2:363224/129‑1 (MQ=255)

       tCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAAc                                                                                                                       >  1:363224/1‑129 (MQ=255)

         gATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGATCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGa                                                                                                           <  2:641184/139‑1 (MQ=255)

           tCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGAtt                                                                                                         >  2:560826/1‑139 (MQ=255)

               aCGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTAccc                                                                                                     >  1:190969/1‑139 (MQ=255)

                     gTCGATATTGAAGTGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGt                                                                                               >  1:810486/1‑139 (MQ=255)

                     gTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGt                                                                                               >  2:696540/1‑139 (MQ=255)

                     gTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGt                                                                                               >  2:867297/1‑139 (MQ=255)

                     gTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGt                                                                                               >  2:900290/1‑139 (MQ=255)

                     gTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGAGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGt                                                                                               >  1:822849/1‑139 (MQ=255)

                             tGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGt                                                                                       <  1:867297/139‑1 (MQ=255)

                               aaGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTgg                                                                                     >  1:576859/1‑139 (MQ=255)

                               aaGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTgg                                                                                     >  1:977470/1‑139 (MQ=255)

                                   gAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCAcc                                                                                 <  2:741743/139‑1 (MQ=255)

                                   gAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCAcc                                                                                 <  1:900290/139‑1 (MQ=255)

                                   gAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCAcc                                                                                 <  2:576859/139‑1 (MQ=255)

                                    aaaGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAAc                                                                                                               >  2:188031/1‑108 (MQ=255)

                                    aaaGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAAc                                                                                                               <  1:188031/108‑1 (MQ=255)

                                    aaaGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGttt                                                                                             <  2:320704/126‑1 (MQ=255)

                                    aaaGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGttt                                                                                             >  1:320704/1‑126 (MQ=255)

                                    aaaGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCa                                                                                <  1:771306/139‑1 (MQ=255)

                                             gatgaATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAaca                                                                       <  1:560826/139‑1 (MQ=255)

                                             gatgaATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAaca                                                                       <  2:598441/139‑1 (MQ=255)

                                                  aTTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATg                                                                  <  2:810486/139‑1 (MQ=255)

                                                     tATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGcac                                                               <  2:190969/139‑1 (MQ=255)

                                                         gAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCGGCGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAact                                                           >  1:588690/1‑139 (MQ=255)

                                                                      tAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTcgc                                              >  2:196729/1‑139 (MQ=255)

                                                                        aGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTa                                                                          <  1:371575/109‑1 (MQ=255)

                                                                        aGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTa                                                                          >  2:371575/1‑109 (MQ=255)

                                                                        aGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACAcc                                                                     >  1:145737/1‑114 (MQ=255)

                                                                        aGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACAcc                                                                     <  2:145737/114‑1 (MQ=255)

                                                                         gcgcCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTCGCGga                                           >  2:849985/1‑139 (MQ=255)

                                                                                          gTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCg                                                      <  1:10585/111‑1 (MQ=255)

                                                                                          gTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCg                                                      >  2:10585/1‑111 (MQ=255)

                                                                                          gTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTCGCGGACGACCGTTCGGtgctgc                          >  1:716145/1‑139 (MQ=255)

                                                                                             aTTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCAcc                                                                                 <  1:352724/81‑1 (MQ=255)

                                                                                             aTTGAGGATGATGTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCAcc                                                                                 >  2:352724/1‑81 (MQ=255)

                                                                                                     tgatgTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTCGCGGACGACCGTTCGGTGCTGCTGGGCg                    <  1:220690/134‑1 (MQ=255)

                                                                                                     tgatgTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTCGCGGACGACCGTTCGGTGCTGCTGGGCg                    >  2:220690/1‑134 (MQ=255)

                                                                                                       atgTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCAt                                                                                                   >  2:152961/1‑53 (MQ=255)

                                                                                                       atgTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCAt                                                                                                   <  1:152961/53‑1 (MQ=255)

                                                                                                       atgTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCa                                                                                   <  2:314618/69‑1 (MQ=255)

                                                                                                       atgTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCa                                                                                   >  1:314618/1‑69 (MQ=255)

                                                                                                       atgTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGca                                                                <  1:659082/88‑1 (MQ=255)

                                                                                                       atgTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGca                                                                >  2:659082/1‑88 (MQ=255)

                                                                                                       atgTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTCGCGGACGACCGTTCGGTGCTGCTGGGCGTCAtgtg             <  1:196729/139‑1 (MQ=255)

                                                                                                       atgTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTCGCGGACGACCGTTCGGTGCTGCTGGGCGTCAtgtg             <  1:696540/139‑1 (MQ=255)

                                                                                                        tgTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATg                                                                  >  2:319334/1‑85 (MQ=255)

                                                                                                        tgTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATg                                                                  <  1:319334/85‑1 (MQ=255)

                                                                                                        tgTAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTCGCGGACGACCGTTCGGTGCTGCTGGGCGTCATGTgc            >  1:1003498/1‑139 (MQ=255)

                                                                                                          tAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTATCCATCAGTTTGCGGGTGGCACCATTGGTAACTCCATGCACAACTACTCGGTGCTCGCGGACGACCGTTCGGTGCTGCTGGGCGTCATGTgcag          <  2:977470/139‑1 (MQ=255)

                                                                                                          tAGCCGAAGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTCGCGGACGACCGTTCGGTGCTGCTGGGCGTCATGTgcag          <  1:849985/139‑1 (MQ=255)

                                                                                                                 aGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCAt                                                                                                   <  2:649288/43‑1 (MQ=255)

                                                                                                                 aGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCAt                                                                                                   >  1:649288/1‑43 (MQ=255)

                                                                                                                 aGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAaca                                                                       <  2:471526/71‑1 (MQ=255)

                                                                                                                 aGCGCTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAaca                                                                       >  1:471526/1‑71 (MQ=255)

                                                                                                                  gcgcTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTCGCGGACGACCGTTCGGTGCTGCTGGGCGTCATGTGCAGCAATAtt   >  1:93093/1‑138 (MQ=255)

                                                                                                                  gcgcTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTCGCGGACGACCGTTCGGTGCTGCTGGGCGTCATGTGCAGCAATAtt   <  2:93093/138‑1 (MQ=255)

                                                                                                                  gcgcTTTATCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTCGCGGACGACCGTTCGGTGCTGCTGGGCGTCATGTGCAGCAATATTg  >  1:403062/1‑139 (MQ=255)

                                                                                                                          tCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTa                                                                          >  2:71455/1‑59 (MQ=255)

                                                                                                                          tCAGGGACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTa                                                                          <  1:71455/59‑1 (MQ=255)

GTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGCCGAAGCGCTTTATCAGGAACTAAAACAGAAAAACCTGATTACCCATCAGTTTGCGGGTGGCACCATTGGTAACACCATGCACAACTACTCGGTGCTCGCGGACGACCGTTCGGTGCTGCTGGGCGTCATGTGCAGCAATATTG  >  NC_000913/500233‑500485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A