Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	571,444	G→A	184 (TAG→TAA)	ybcL →	inactive polymorphonuclear leukocyte migration suppressor, DLP12 prophage, UPF0098 family secreted protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	571,444	0	G	A	100.0%	128.3 / NA	40	184 (TAG→TAA)	ybcL	inactive polymorphonuclear leukocyte migration suppressor, DLP12 prophage, UPF0098 family secreted protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/20); total (20/20)

CCAGTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAGGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTTAT  >  NC_000913/571309‑571578

ccAGTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGt                                                                                                                                     >  1:825009/1‑139 (MQ=255)

    tttAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTgaga                                                                                                                                 <  1:246637/139‑1 (MQ=255)

              gggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGc                                                                                                                       >  2:955658/1‑139 (MQ=255)

              gggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGc                                                                                                                       >  2:358182/1‑139 (MQ=255)

              gggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGc                                                                                                                       >  1:988499/1‑139 (MQ=255)

               ggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      <  2:988499/139‑1 (MQ=255)

                  tctAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      >  2:475741/1‑136 (MQ=255)

                  tctAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      <  1:475741/136‑1 (MQ=255)

                     aaaaaCTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGt                                                                                                                <  2:356052/139‑1 (MQ=255)

                          cTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGAc                                                                                                           >  1:700568/1‑139 (MQ=255)

                              aaaGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGTTGGCAAGAGGTAAGACTAAc                                                                                                       >  2:190571/1‑139 (MQ=255)

                                      cTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGAt                                                                                               <  1:89948/139‑1 (MQ=255)

                                         tAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCga                                                                                            <  2:825009/139‑1 (MQ=255)

                                              tCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGa                                                                                                            <  2:467533/118‑1 (MQ=255)

                                              tCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGa                                                                                                            >  1:467533/1‑118 (MQ=255)

                                                  aCTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACg                                                                                   <  2:700568/139‑1 (MQ=255)

                                                        gCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGt                                                                                                                                     <  2:664013/83‑1 (MQ=255)

                                                        gCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGt                                                                                                                                     >  1:664013/1‑83 (MQ=255)

                                                              cGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAAca                                                                       >  2:57913/1‑139 (MQ=255)

                                                                  aGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAAcatcat                                                                   >  2:950956/1‑139 (MQ=255)

                                                                              gCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAgg                                                       >  2:338486/1‑139 (MQ=255)

                                                                                ttAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGt                                                     <  2:906514/139‑1 (MQ=255)

                                                                                  aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACg                                                                                   >  1:633435/1‑107 (MQ=255)

                                                                                  aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACg                                                                                   <  2:633435/107‑1 (MQ=255)

                                                                                  aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAg                                                        <  2:787126/134‑1 (MQ=255)

                                                                                  aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAg                                                        >  1:787126/1‑134 (MQ=255)

                                                                                  aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTa                                                   >  2:89672/1‑139 (MQ=255)

                                                                                  aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTa                                                   >  1:639513/1‑139 (MQ=255)

                                                                                    tGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAg                                                 <  1:950956/139‑1 (MQ=255)

                                                                                              aTCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATga                                                                 <  1:421124/113‑1 (MQ=255)

                                                                                              aTCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATga                                                                 >  2:421124/1‑113 (MQ=255)

                                                                                              aTCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGc                                       <  1:89672/139‑1 (MQ=255)

                                                                                                        cTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTtgtg                             <  2:530030/139‑1 (MQ=255)

                                                                                                        cTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTtgtg                             <  1:338486/139‑1 (MQ=255)

                                                                                                                    cAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCa                 <  2:79445/139‑1 (MQ=255)

                                                                                                                     aGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAg                >  1:315586/1‑139 (MQ=255)

                                                                                                                       tttATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGAt                                                                                >  2:597904/1‑73 (MQ=255)

                                                                                                                       tttATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGAt                                                                                <  1:597904/73‑1 (MQ=255)

                                                                                                                                tAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGt     >  2:118287/1‑139 (MQ=255)

                                                                                                                                   aGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTTAt  <  2:639513/139‑1 (MQ=255)

CCAGTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAGGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTTAT  >  NC_000913/571309‑571578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A