Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	872,801	G→A	304 (TAG→TAA)	gsiD →	glutathione ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	872,801	0	G	A	100.0%	90.1 / NA	29	304 (TAG→TAA)	gsiD	glutathione ABC transporter permease
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/14); total (15/14)

TGAGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGG  >  NC_000913/872666‑872924

tGAGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTa                                                                                                                          <  1:234166/139‑1 (MQ=255)

tGAGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTa                                                                                                                          <  2:953062/139‑1 (MQ=255)

   ggCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGt                                                                                                                       >  2:248987/1‑139 (MQ=255)

     cTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGttt                                                                                                                     >  2:251893/1‑139 (MQ=255)

        gAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGaa                                                                                                                  <  2:107227/139‑1 (MQ=255)

          gCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAata                                                                                                                >  1:395452/1‑139 (MQ=255)

                            ccgcATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATc                                                                                              >  2:710250/1‑139 (MQ=255)

                             cgcATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCa                                                                                             >  2:5782/1‑139 (MQ=255)

                             cgcATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCa                                                                                             >  2:763572/1‑139 (MQ=255)

                                aTGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCt                                                                                          <  1:763572/139‑1 (MQ=255)

                                             tCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAattat                                                                             <  2:395452/139‑1 (MQ=255)

                                             tCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAattat                                                                             <  1:902206/139‑1 (MQ=255)

                                                   ccTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCt                                                                       >  1:360995/1‑139 (MQ=255)

                                                    cTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTa                                                                      <  1:251893/139‑1 (MQ=255)

                                                      ggCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAg                                                                    >  2:764189/1‑139 (MQ=255)

                                                       gCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGc                                                                   <  1:764189/139‑1 (MQ=255)

                                                        cTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCa                                                                  <  1:5782/139‑1 (MQ=255)

                                                                     cGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACtt                                                     >  2:602552/1‑139 (MQ=255)

                                                                             cGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCaa                                                                 <  2:996950/119‑1 (MQ=255)

                                                                             cGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCaa                                                                 >  1:996950/1‑119 (MQ=255)

                                                                             cGTTCAATTTGTAGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCATAAGCAAGTGTTGTAACTTTCTGCTGa                                             <  1:602552/139‑1 (MQ=255)

                                                                               ttCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGccc                                                                        >  1:682372/1‑110 (MQ=255)

                                                                               ttCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGAtt                                           >  2:673284/1‑139 (MQ=255)

                                                                               ctCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAAGTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGccc                                                                        <  2:682372/109‑1 (MQ=255)

                                                                                  aaTTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTg                                        <  1:968653/139‑1 (MQ=255)

                                                                                                           aTGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTaaaaa               >  1:799217/1‑139 (MQ=255)

                                                                                                            tGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTctgc                                                <  2:693950/105‑1 (MQ=255)

                                                                                                            tGCGCTGGATCCGAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTctgc                                                >  1:693950/1‑105 (MQ=255)

                                                                                                                        gAAAATTAAAGGATAATTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAggg  >  1:727799/1‑139 (MQ=255)

TGAGGCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTTCTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAAATTAAAGGATAGTTACGTTTGAATATTGCTTGAAAGGGTAATCACCTCACAGGAAATTATTGCCCTAAGCAAGTGTTGTAACTTTCTGCTGATTTTGTAGAATCGGGTAATTTGGTTAAAAAGCCGCAGCAAGGG  >  NC_000913/872666‑872924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A