Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,189,703	A→G	V25A (GTA→GCA)	phoQ ←	sensory histidine kinase in two‑component regulatory system with PhoP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,189,703	0	A	G	100.0%	76.2 / NA	22	V25A (GTA→GCA)	phoQ	sensory histidine kinase in two‑component regulatory system with PhoP
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/9); total (13/9)

GTTTTCCCACTTCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTT  >  NC_000913/1189576‑1189828

gTTTTCCCACTTCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGcc                                                                                                                    <  2:4525/139‑1 (MQ=255)

           tCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAg                                                                                                         >  2:966376/1‑139 (MQ=255)

           tCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAg                                                                                                         >  2:17763/1‑139 (MQ=255)

                      taGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTg                                                                                                                      >  1:147272/1‑115 (MQ=255)

                      taGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTg                                                                                                                      <  2:147272/115‑1 (MQ=255)

                             gATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCga                                                                                       >  2:520066/1‑139 (MQ=255)

                                  ctctCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGaaaa                                                                                                     >  1:296542/1‑120 (MQ=255)

                                  ctctCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGaaaa                                                                                                     <  2:296542/120‑1 (MQ=255)

                                  ctctCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCg                                                                                  >  2:747437/1‑139 (MQ=255)

                                  ctctCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCg                                                                                  <  1:17763/139‑1 (MQ=255)

                                                      aaaCGTAGTTTTATCGAAACTGACTCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAAt                                                              >  1:684679/1‑139 (MQ=255)

                                                                  aTCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGATGCAGTAATTTTTTCATCAgc                                                  <  2:684679/139‑1 (MQ=255)

                                                                                           aGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGcc                         <  1:966376/139‑1 (MQ=255)

                                                                                                gACCATTCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAAGTCGAACAGATAGCCCTGGCCGCGaa                    >  1:690091/1‑139 (MQ=255)

                                                                                                     ttCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACggtg               >  1:11497/1‑139 (MQ=255)

                                                                                                      tCCGTAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACggtgg              >  1:417864/1‑139 (MQ=255)

                                                                                                          tAGGCAAGCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAAt          <  1:747437/139‑1 (MQ=255)

                                                                                                                 gCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACggtg               >  2:310490/1‑127 (MQ=255)

                                                                                                                 gCGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACggtg               <  1:310490/127‑1 (MQ=255)

                                                                                                                  cGAAAGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCActtctt  >  1:514818/1‑139 (MQ=255)

                                                                                                                      aGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTtcat                                                      <  2:215771/83‑1 (MQ=255)

                                                                                                                      aGCACCAGTGCTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTtcat                                                      >  1:215771/1‑83 (MQ=255)

GTTTTCCCACTTCGCAAGGGTATAGAACAGATTGCTCTCGCCACGTAACAGCCGAAACGTAGTTTTATCGAAACTGACGCTATAACCGATCAGCGCGACCATTCCGTAGGCAAGCGAAAGCACCAGTACTACCGCTGCCGTTGCCAACAGAAAACGTACCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTT  >  NC_000913/1189576‑1189828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A