Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I2 R1 764 42.0 2018750 95.5% 1927906 109.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,594,132 (C)8→9 pseudogene (3856/3861 nt) yneO ← pseudogene, AidA homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,594,1241.C92.1% 120.5 / 3.6 38pseudogene (2/1461 nt)yneOpseudogene, AidA homolog
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base C (17/18);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

CCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCC  >  NC_000913/1594007‑1594251
                                                                                                                      |                                                                                                                               
ccGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCAt                                                                                                                   <  1:280388/133‑1 (MQ=255)
ccGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCAt                                                                                                                   >  2:280388/1‑133 (MQ=255)
     ataGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCatat                                                                                                        >  1:740033/1‑139 (MQ=255)
           gCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTg                                                                                                  <  1:208260/139‑1 (MQ=255)
           gCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTg                                                                                                  <  1:379215/139‑1 (MQ=255)
                 aaCGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATATTGGTTTTAGCGACACGCAGTG‑ccc                                                                                                                              <  1:684440/104‑1 (MQ=255)
                 aaCGGTCAGTACATTCCCCGCTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑ccc                                                                                                                              >  2:684440/1‑104 (MQ=255)
                            cATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTccc                                                                                 <  1:178240/139‑1 (MQ=255)
                                  ccGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATc                                                                           >  1:828389/1‑139 (MQ=255)
                                    ggTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATcac                                                                         >  2:871820/1‑139 (MQ=255)
                                      tttCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCATGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCacac                                                                       <  1:428838/139‑1 (MQ=255)
                                      tttCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCacac                                                                       >  1:775861/1‑139 (MQ=255)
                                      tttCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCacac                                                                       <  2:775861/139‑1 (MQ=255)
                                             ccTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGtt                                                                <  2:828389/139‑1 (MQ=255)
                                              cTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTa                                                               <  1:871820/139‑1 (MQ=255)
                                                      ttCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑cc                                                                                                                               <  1:786142/66‑1 (MQ=255)
                                                      ttCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑cc                                                                                                                               >  2:786142/1‑66 (MQ=255)
                                                             gAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATGACGTTCat                                                                                                          <  2:872825/81‑1 (MQ=255)
                                                             gAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATGACGTTCat                                                                                                          >  1:872825/1‑81 (MQ=255)
                                                                ccGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTg                                                                                                  <  2:529247/86‑1 (MQ=255)
                                                                ccGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTg                                                                                                  >  1:529247/1‑86 (MQ=255)
                                                                ccGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTg                                                                                                  >  1:228146/1‑86 (MQ=255)
                                                                ccGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTg                                                                                                  <  2:228146/86‑1 (MQ=255)
                                                                   tGCCGCCATTCTCCAGGTTGCCGCCGCTAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCacac                                                                       <  1:636587/110‑1 (MQ=255)
                                                                   tGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCacac                                                                       >  2:167362/1‑110 (MQ=255)
                                                                   tGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCacac                                                                       <  1:167362/110‑1 (MQ=255)
                                                                   tGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCacac                                                                       >  2:636587/1‑110 (MQ=255)
                                                                   tGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACtt                                          >  2:146631/1‑139 (MQ=255)
                                                                   tGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑cccccccc                                                                                                                         <  1:313878/59‑1 (MQ=255)
                                                                   tGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑cccccccc                                                                                                                         >  2:313878/1‑59 (MQ=255)
                                                                             ctcCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCg                                                                     >  2:142291/1‑102 (MQ=255)
                                                                             ctcCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCg                                                                     <  1:142291/102‑1 (MQ=255)
                                                                                   gTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCag                          <  2:740033/139‑1 (MQ=255)
                                                                                      gccgccGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGt                       >  2:39285/1‑139 (MQ=255)
                                                                                       ccgccgATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTc                      >  1:534768/1‑139 (MQ=255)
                                                                                       ccgccgATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGc                            >  2:246302/1‑132 (MQ=255)
                                                                                       ccgccgATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGGGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGc                            <  1:246302/132‑1 (MQ=255)
                                                                                        cgccgATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAAtgtg                                                 <  2:185540/111‑1 (MQ=255)
                                                                                        cgccgATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAAtgtg                                                 >  1:185540/1‑111 (MQ=255)
                                                                                        cgccgATAGTGGTTTTAGCGACACGCAGTG‑ccccccccc                                                                                                                        <  2:845088/39‑2 (MQ=255)
                                                                                        cgccgATAGTGGTTTTAGCGACACGCAGTG‑ccccccccc                                                                                                                        >  1:845088/1‑38 (MQ=255)
                                                                                            gATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAAcc                >  1:424771/1‑139 (MQ=255)
                                                                                                           gACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGTAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTccc  <  2:310192/139‑1 (MQ=255)
                                                                                                           gACACGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTccc  >  1:992328/1‑139 (MQ=255)
                                                                                                             cacGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGcc                                       >  2:94994/1‑100 (MQ=255)
                                                                                                             cacGCAGTGCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGcc                                       <  1:94994/100‑1 (MQ=255)
                                                                                                                      |                                                                                                                               
CCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCCTTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCC  >  NC_000913/1594007‑1594251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A