Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,179,330	T→C	S170S (AGT→AGC)	yegT →	nucleoside transporter, low affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,179,330	0	T	C	100.0%	133.9 / NA	37	S170S (AGT→AGC)	yegT	nucleoside transporter, low affinity
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/15); total (22/15)

CGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTAT‑GCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTTT  >  NC_000913/2179205‑2179464

cgcATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTAT‑GCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCg                                                                                                                           <  1:385621/139‑1 (MQ=255)

               ggCACTATCGGCTGGTTTCCCTCGGGACT‑GCCTGGGGTTTTCTGCCGGCAATAACGGGGTATGGCCAATTCTTAACCGCTTACCATCCCGCGGCTGATTACCGCCGAAAGTTTGGTTCTGCTGGTGGTGTTGCCGtttt                                                                                                            >  1:697192/1‑139 (MQ=255)

               ggCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTAT‑GCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGtttt                                                                                                            >  2:1006147/1‑139 (MQ=255)

                      tCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTAT‑GCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGcc                                                                                                     >  1:859668/1‑139 (MQ=255)

                                            ggCATGTGGTTTCTTGCCGCAAATACTGGGGTAT‑GCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGcca                                                                                           <  2:788253/127‑1 (MQ=255)

                                            ggCATGTGGTTTCTTGCCGCAAATACTGGGGTAT‑GCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGcca                                                                                           >  1:788253/1‑127 (MQ=255)

                                                       tCTTGCCGCAAATACTGGGGTAT‑GCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGa                                                                    <  1:799135/139‑1 (MQ=255)

                                                            ccGCAAATACTGGGGTAT‑GCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTa                                                               >  1:905579/1‑139 (MQ=255)

                                                                     cTGGGGTAT‑GCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGc                                                      <  1:1006147/139‑1 (MQ=255)

                                                                      tGGGGTAT‑GCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                     <  2:697192/139‑1 (MQ=255)

                                                                      tGGGGTAT‑GCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                     <  1:940879/139‑1 (MQ=255)

                                                                             t‑GCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTg                                              >  2:212549/1‑139 (MQ=255)

                                                                                ccGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGa                                            >  2:757588/1‑139 (MQ=255)

                                                                                    tatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGaca                                                                                                <  2:275889/83‑1 (MQ=255)

                                                                                    tatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGaca                                                                                                >  1:275889/1‑83 (MQ=255)

                                                                                     atCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGa                                            <  1:686230/134‑1 (MQ=255)

                                                                                     atCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGa                                            >  2:686230/1‑134 (MQ=255)

                                                                                          aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                             >  2:914256/1‑80 (MQ=255)

                                                                                          aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                             <  1:914256/80‑1 (MQ=255)

                                                                                          aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATc                                  >  2:931013/1‑139 (MQ=255)

                                                                                             gACTAACATCCCGCTGCTGATTACCGCCGGAAGctctgctctg                                                                                                                               >  2:502069/1‑43 (MQ=255)

                                                                                             gACTAACATCCCGCTGCTGATTACCGCCGGAAGctctgctctg                                                                                                                               <  1:502069/43‑1 (MQ=255)

                                                                                              aCTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                             <  2:349707/76‑1 (MQ=255)

                                                                                              aCTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                             >  1:349707/1‑76 (MQ=255)

                                                                                              aCTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctgc                              >  1:67114/1‑139 (MQ=255)

                                                                                               cTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCctgct                             >  2:275824/1‑139 (MQ=255)

                                                                                                 aaCATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCtgctgc                           <  1:275824/139‑1 (MQ=255)

                                                                                                          ctgctgATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                             <  1:753328/64‑1 (MQ=255)

                                                                                                          ctgctgATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                             >  2:753328/1‑64 (MQ=255)

                                                                                                          ctgctgATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATaaaa                  >  1:739021/1‑139 (MQ=255)

                                                                                                             ctgATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAAt                                                                       <  2:241664/83‑1 (MQ=255)

                                                                                                             ctgATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAAt                                                                       >  1:241664/1‑83 (MQ=255)

                                                                                                              tgATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACtt              >  2:100223/1‑139 (MQ=255)

                                                                                                                aTTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTcc            >  1:532097/1‑139 (MQ=255)

                                                                                                                aTTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTcc            <  2:67114/139‑1 (MQ=255)

                                                                                                                 ttACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCt           <  1:534012/139‑1 (MQ=255)

                                                                                                                     cgccgGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTc       >  1:44825/1‑139 (MQ=255)

                                                                                                                     cgccgGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTc       >  2:301411/1‑139 (MQ=255)

                                                                                                                        cgGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCttt    >  2:632475/1‑139 (MQ=255)

                                                                                                                          gAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCttttt  >  2:503218/1‑139 (MQ=255)

CGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTAT‑GCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTTT  >  NC_000913/2179205‑2179464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A