Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,521,852	T→C	D209G (GAT→GGT)	xapR ←	transcriptional activator of xapAB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,521,852	0	T	C	100.0%	162.8 / NA	47	D209G (GAT→GGT)	xapR	transcriptional activator of xapAB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/27); total (20/27)

TTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACC  >  NC_000913/2521719‑2521981

ttGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcag                                                                                                                              <  1:940685/139‑1 (MQ=255)

 tgtgCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTTGCAATCTCGCTGCAAAAAACCCcagt                                                                                                                             <  2:697153/139‑1 (MQ=255)

 tgtgCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagt                                                                                                                             <  1:994549/139‑1 (MQ=255)

                   aTCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCa                                                                                                           <  1:639432/139‑1 (MQ=255)

                    tCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCAt                                                                                                          <  1:879128/139‑1 (MQ=255)

                       aTGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGt                                                                                                       >  2:518308/1‑139 (MQ=255)

                              aCCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACaaaa                                                                                                <  2:985113/139‑1 (MQ=255)

                                 aaTGCCCATGCTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATAc                                                                                             <  1:692053/139‑1 (MQ=255)

                                        aTACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCAc                                                                                      >  1:496056/1‑139 (MQ=255)

                                         tACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACg                                                                                     >  1:100846/1‑139 (MQ=255)

                                             gACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCa                                                                                                           <  1:940452/113‑1 (MQ=255)

                                             gACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCa                                                                                                           >  2:940452/1‑113 (MQ=255)

                                              aCCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCca                                                                                                                               >  2:229995/1‑92 (MQ=255)

                                              aCCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCca                                                                                                                               <  1:229995/92‑1 (MQ=255)

                                               ccATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAgcgc                                                                               >  2:699761/1‑139 (MQ=255)

                                                cATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCt                                                                              <  2:191992/139‑1 (MQ=255)

                                                          aCCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagtca                                                                                                                           <  2:806971/84‑1 (MQ=255)

                                                          aCCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagtca                                                                                                                           >  1:806971/1‑84 (MQ=255)

                                                          aCCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagt                                                                                                                             >  1:720760/1‑82 (MQ=255)

                                                          aCCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagt                                                                                                                             <  2:720760/82‑1 (MQ=255)

                                                          aCCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACt                                                                                            >  1:568990/1‑115 (MQ=255)

                                                          aCCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACt                                                                                            <  2:568990/115‑1 (MQ=255)

                                                          aCCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGa                                                                    <  2:464402/139‑1 (MQ=255)

                                                            cGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACg                                                                  <  1:518308/139‑1 (MQ=255)

                                                             gTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACgg                                                                 >  2:375651/1‑139 (MQ=255)

                                                              tttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGt                                                                                    <  2:519140/119‑1 (MQ=255)

                                                              tttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGt                                                                                    >  1:519140/1‑119 (MQ=255)

                                                                      tCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAatg                                                        <  1:286395/139‑1 (MQ=255)

                                                                      tCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAatg                                                        >  1:839385/1‑139 (MQ=255)

                                                                                 gcgAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAAcccc                                                                                                                                >  1:815739/1‑56 (MQ=255)

                                                                                 gcgAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAAcccc                                                                                                                                <  2:815739/56‑1 (MQ=255)

                                                                                  cgAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAgatgat                                            <  2:839385/139‑1 (MQ=255)

                                                                                   gAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGatgatg                                           <  2:100846/139‑1 (MQ=255)

                                                                                   gAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGatgatg                                           <  2:496056/139‑1 (MQ=255)

                                                                                             cGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGAc                                                                   <  1:471395/105‑1 (MQ=255)

                                                                                             cGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGAc                                                                   >  2:471395/1‑105 (MQ=255)

                                                                                               gTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCca                                                                                                                               >  2:200262/1‑43 (MQ=255)

                                                                                               gTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCca                                                                                                                               <  1:200262/43‑1 (MQ=255)

                                                                                               gTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGatgatg                                           >  2:436553/1‑127 (MQ=255)

                                                                                               gTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGatgatg                                           <  1:436553/127‑1 (MQ=255)

                                                                                               gTGAAAATACCACCTGCTACGATACTCTCTGCAAAAAACTTCTCTCACGGTAAACGCGCGGCATTGTAACAAAATACGTCTCTCGTACAGATTACACTCGGACGGTGGAAAATGATGAGTGATGCTGCTCTTccgcaat                               >  1:752165/1‑133 (MQ=255)

                                                                                                  aaaaTCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCAt                                                                                                          <  2:583349/61‑1 (MQ=255)

                                                                                                  aaaaTCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCAt                                                                                                          >  1:583349/1‑61 (MQ=255)

                                                                                                    aaTCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCGTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGcca                          >  2:667526/1‑139 (MQ=255)

                                                                                                                 ggCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATAc                                                                                             >  2:1006528/1‑59 (MQ=255)

                                                                                                                 ggCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATAc                                                                                             <  1:1006528/59‑1 (MQ=255)

                                                                                                                  gCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGAt            <  1:699761/139‑1 (MQ=255)

                                                                                                                       cTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATg       <  1:375651/139‑1 (MQ=255)

                                                                                                                            tgaaaaaaaCCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAAcc  >  2:245812/4‑139 (MQ=255)

TTGTGCGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACC  >  NC_000913/2521719‑2521981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A