Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,697,915	C→T	212 (TAG→TAA)	pgpC ←	phosphatidylglycerophosphatase C, membrane bound

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,697,915	0	C	T	100.0%	112.3 / NA	36	212 (TAG→TAA)	pgpC	phosphatidylglycerophosphatase C, membrane bound
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/19); total (17/19)

AGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGAT  >  NC_000913/2697791‑2698037

aGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTg                                                                                                              <  2:759564/139‑1 (MQ=255)

           gCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgc                                                                                                   <  2:926750/139‑1 (MQ=255)

                    aGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTa                                                                                          >  1:35343/1‑139 (MQ=255)

                           gACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCa                                                                                   >  1:290151/1‑139 (MQ=255)

                                     gTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGa                                                                         <  2:290151/139‑1 (MQ=255)

                                      tATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGAc                                                                        <  1:800591/139‑1 (MQ=255)

                                           gTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGaaa                                                                   >  2:663974/1‑139 (MQ=255)

                                                tAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAag                                                              >  2:914640/1‑139 (MQ=255)

                                                            cAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTc                                                  >  2:158245/1‑139 (MQ=255)

                                                            cAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTc                                                  <  2:35343/139‑1 (MQ=255)

                                                                 aaaaaGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGAt                                             <  2:100315/139‑1 (MQ=255)

                                                                     aGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACt                                         <  1:663974/139‑1 (MQ=255)

                                                                      gAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTg                                        <  1:168538/139‑1 (MQ=255)

                                                                      gAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTg                                        <  1:158245/139‑1 (MQ=255)

                                                                          aCTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGc                                    >  1:425572/1‑139 (MQ=255)

                                                                           cTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACt                                                                                        >  1:257937/1‑86 (MQ=255)

                                                                           cTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACt                                                                                        <  2:257937/86‑1 (MQ=255)

                                                                              cGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGt                                                                                            >  1:837786/1‑79 (MQ=255)

                                                                              cGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGt                                                                                            <  2:837786/79‑1 (MQ=255)

                                                                                          cgcgGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGa                                                                         <  1:770720/86‑1 (MQ=255)

                                                                                          cgcgGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGa                                                                         >  2:770720/1‑86 (MQ=255)

                                                                                          cgcgGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGa                                                                         <  1:322421/86‑1 (MQ=255)

                                                                                          cgcgGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGa                                                                         >  2:322421/1‑86 (MQ=255)

                                                                                          cgcgGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACt                                         <  1:867345/118‑1 (MQ=255)

                                                                                          cgcgGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACt                                         >  2:867345/1‑118 (MQ=255)

                                                                                               cgcATTATACACGGACGCTATGCTTTACTTTATTcc                                                                                                                      <  1:906430/36‑1 (MQ=255)

                                                                                               cgcATTATACACGGACGCTATGCTTTACTTTATTcc                                                                                                                      >  2:906430/1‑36 (MQ=255)

                                                                                                            gACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTAc                      >  2:839952/1‑119 (MQ=255)

                                                                                                            gACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTAc                      <  1:839952/119‑1 (MQ=255)

                                                                                                            gACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGAt  >  2:921021/1‑139 (MQ=255)

                                                                                                              cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCa                                                                                                       >  2:522907/1‑36 (MQ=255)

                                                                                                              cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCa                                                                                                       <  1:522907/36‑1 (MQ=255)

                                                                                                              cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                  <  2:598790/41‑1 (MQ=255)

                                                                                                              cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                  >  1:598790/1‑41 (MQ=255)

                                                                                                                         aCTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGc                              >  1:451447/1‑98 (MQ=255)

                                                                                                                         aCTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGc                              <  2:451447/98‑1 (MQ=255)

AGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGAT  >  NC_000913/2697791‑2698037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A