Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I2 R1 760 52.7 2744488 90.2% 2475528 105.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,863,004 T→C R78R (CGT→CGC)  gapA → glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,863,0040TC98.3% 206.0 / NA 58R78R (CGT→CGC) gapAglyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (29/28);  minor base G (1/0);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGT  >  NC_000913/1862869‑1863137
                                                                                                                                       |                                                                                                                                     
cGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGAt                                                                                                                                    >  2:1337272/1‑139 (MQ=255)
     tGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGc                                                                                                                               <  1:539390/139‑1 (MQ=255)
         aGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAAc                                                                                                                           <  1:688003/139‑1 (MQ=255)
                 aTTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATg                                                                                                                   >  1:539686/1‑139 (MQ=255)
                     cATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGAc                                                                                                               >  2:61718/1‑139 (MQ=255)
                       tGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGa                                                                                                             >  2:579141/1‑139 (MQ=255)
                           aTACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGaaa                                                                                                                     >  2:360072/1‑127 (MQ=255)
                           aTACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGaaa                                                                                                                     <  1:360072/127‑1 (MQ=255)
                             aCATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGa                                                                                                                >  1:670234/1‑130 (MQ=255)
                             aCATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGa                                                                                                                <  2:670234/130‑1 (MQ=255)
                                tGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGgtgt                                                                                                    >  2:497648/1‑139 (MQ=255)
                                 gCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCgg                                                                                                                                >  2:1266718/1‑110 (MQ=255)
                                 gCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCgg                                                                                                                                <  1:1266718/110‑1 (MQ=255)
                                   tGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGa                                                                                                 >  2:289703/1‑139 (MQ=255)
                                       atatGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGtt                                                                                             >  2:154954/1‑139 (MQ=255)
                                            aCTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGc                                                                                        >  2:1016989/1‑139 (MQ=255)
                                                cACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGaa                                                                                    >  2:269286/1‑139 (MQ=255)
                                                 aCTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAg                                                                                   <  2:1351956/139‑1 (MQ=255)
                                                  cTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGc                                                                                  >  1:1226066/1‑139 (MQ=255)
                                                    cACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCaa                                                                                <  1:497648/139‑1 (MQ=255)
                                                    cACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCaa                                                                                <  2:1133137/139‑1 (MQ=255)
                                                     aCGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAAc                                                                               <  1:579141/139‑1 (MQ=255)
                                                       ggCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGATACCGCTGAACGCGATCCGGCTa                                                                                                                             <  2:1210731/91‑1 (MQ=255)
                                                       ggCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGATACCGCTGAACGCGATCCGGCTa                                                                                                                             >  1:1210731/1‑91 (MQ=255)
                                                        gCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAAt                                                                                                                    <  2:393726/99‑1 (MQ=255)
                                                        gCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAAt                                                                                                                    >  1:393726/1‑99 (MQ=255)
                                                            tttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACGGCTGAACGGGATCCGGCTAACCGGAATTGGAACAAATTTGGGTTTGACGTGGTGGCTAAAGAAATTggccgg                                                                        >  2:226207/1‑135 (MQ=255)
                                                            tttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGt                                                                                                          <  1:816071/105‑1 (MQ=255)
                                                            tttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGt                                                                                                          >  2:816071/1‑105 (MQ=255)
                                                                     tACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCctgact                                                               >  1:1266025/1‑139 (MQ=255)
                                                                          ttGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  2:529789/122‑1 (MQ=255)
                                                                          ttGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           >  1:529789/1‑122 (MQ=255)
                                                                            gAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGaaa                                                        <  1:269286/139‑1 (MQ=255)
                                                                               gTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTg                                                     >  1:628409/1‑139 (MQ=255)
                                                                                tGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGc                                                    <  2:1226066/139‑1 (MQ=255)
                                                                                       cGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTaaa                                             <  2:628409/139‑1 (MQ=255)
                                                                                          tCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  1:869317/106‑1 (MQ=255)
                                                                                          tCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           >  2:869317/1‑106 (MQ=255)
                                                                                          tCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAacac                                          >  2:750234/1‑139 (MQ=255)
                                                                                            aTCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACAt                                        >  2:725662/1‑139 (MQ=255)
                                                                                            aTCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTCGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACAt                                        >  1:120535/1‑139 (MQ=255)
                                                                                                       aaCGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTg                             <  1:154954/139‑1 (MQ=255)
                                                                                                        aCGGTAAAAAAATCCGTTTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGtt                                                                                                         <  1:966835/62‑1 (MQ=255)
                                                                                                        aCGGTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGtt                                                                                                         >  2:966835/1‑62 (MQ=255)
                                                                                                           gTAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCgaa                         >  2:520193/1‑139 (MQ=255)
                                                                                                              aaaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGaaa                                                        >  1:777684/1‑105 (MQ=255)
                                                                                                              aaaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGaaa                                                        <  2:777684/105‑1 (MQ=255)
                                                                                                               aaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGaaa                     <  1:557211/139‑1 (MQ=255)
                                                                                                                     ccGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGtt               >  1:683958/1‑139 (MQ=255)
                                                                                                                      cGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGTAACTGGTCTGTTCCTGACTGACGAAACCGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTa              >  1:1283576/1‑139 (MQ=255)
                                                                                                                        tgtTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTCGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATg            <  2:120535/139‑1 (MQ=255)
                                                                                                                              cGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGt      <  2:683958/139‑1 (MQ=255)
                                                                                                                               gCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTc     <  2:1084942/139‑1 (MQ=255)
                                                                                                                               gCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTc     <  1:61718/139‑1 (MQ=255)
                                                                                                                               gCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTc     <  1:289703/139‑1 (MQ=255)
                                                                                                                                  gcaCGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGCCTGCCGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGt  <  1:1143255/137‑1 (MQ=255)
                                                                                                                                  gAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGt  <  2:539686/139‑1 (MQ=255)
                                                                                                                                  gAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGt  >  1:600177/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CGACCTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGT  >  NC_000913/1862869‑1863137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A