Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,218,207	C→T	A117A (GCG→GCA)	yehY ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,218,207	0	C	T	100.0%	151.1 / NA	47	A117A (GCG→GCA)	yehY	putative ABC transporter permease
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/21); total (26/21)

GAGATCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGCGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAGT  >  NC_000913/2218078‑2218336

gagaTCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                          <  2:113251/139‑1 (MQ=255)

       ttAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                          <  2:942153/132‑1 (MQ=255)

       ttAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                          >  1:942153/1‑132 (MQ=255)

       ttAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGa                                                                                                                   >  2:159263/1‑139 (MQ=255)

             gTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCAc                                                                                                             >  1:1197517/1‑139 (MQ=255)

              tGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACt                                                                                                            >  1:638361/1‑139 (MQ=255)

              tGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACt                                                                                                            >  2:1098725/1‑139 (MQ=255)

               gCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTg                                                                                                           >  2:34276/1‑139 (MQ=255)

                ccGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGc                                                                                                          <  2:638361/139‑1 (MQ=255)

                      tACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGc                                                                                                    <  1:34276/139‑1 (MQ=255)

                        cAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTg                                                                                                  >  1:818539/1‑139 (MQ=255)

                             aCCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTAcg                                                                                             >  1:346119/1‑139 (MQ=255)

                                acaGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                          <  2:853657/107‑1 (MQ=255)

                                acaGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                          >  1:853657/1‑107 (MQ=255)

                                acaGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACgcgc                                                                                          >  2:720060/1‑139 (MQ=255)

                                         taatGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                          <  1:883215/98‑1 (MQ=255)

                                         taatGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                          >  2:883215/1‑98 (MQ=255)

                                                   cTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTgc                                                                       >  1:1347044/1‑139 (MQ=255)

                                                              acaacCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGt                                                            >  2:1201500/1‑139 (MQ=255)

                                                                aacCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTcg                                                          >  1:692727/1‑139 (MQ=255)

                                                                      cgcCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCa                                                                                   <  1:872677/108‑1 (MQ=255)

                                                                      cgcCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCa                                                                                   >  2:872677/1‑108 (MQ=255)

                                                                       gcCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCtt                                                   >  2:479510/1‑139 (MQ=255)

                                                                           caGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCa                                               >  1:763196/1‑139 (MQ=255)

                                                                            aGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAg                                              <  2:1197517/139‑1 (MQ=255)

                                                                                 aTGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTccc                                         <  2:818539/139‑1 (MQ=255)

                                                                                   gCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTc                                           <  1:602004/135‑1 (MQ=255)

                                                                                   gCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTc                                           >  2:602004/1‑135 (MQ=255)

                                                                                    cGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCcac                                      <  1:594459/139‑1 (MQ=255)

                                                                                        gagaTTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCa                                  >  2:878213/1‑139 (MQ=255)

                                                                                              cggcggATGGCATCGCTACAGGCCAGCAATGCCAgtgc                                                                                                                                 <  2:258411/38‑1 (MQ=255)

                                                                                              cggcggATGGCATCGCTACAGGCCAGCAATGCCAgtgc                                                                                                                                 >  1:258411/1‑38 (MQ=255)

                                                                                                gcggATGGCATCGCTACAGGCCAGGAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACg                          <  2:763196/139‑1 (MQ=255)

                                                                                                            gCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTgc              <  1:159263/139‑1 (MQ=255)

                                                                                                                    ccAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAAt      <  1:1098725/139‑1 (MQ=255)

                                                                                                                    ccAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAAt      <  1:878213/139‑1 (MQ=255)

                                                                                                                    ccAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAAt      <  1:720060/139‑1 (MQ=255)

                                                                                                                       gCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAg   <  2:97158/139‑1 (MQ=255)

                                                                                                                        cAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTc                                                           >  2:77659/1‑82 (MQ=255)

                                                                                                                        cAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTc                                                           <  1:77659/82‑1 (MQ=255)

                                                                                                                        cAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTccc                                         <  2:636847/100‑1 (MQ=255)

                                                                                                                        cAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTccc                                         >  1:636847/1‑100 (MQ=255)

                                                                                                                        cAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTc                                           >  2:83732/1‑98 (MQ=255)

                                                                                                                        cAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTc                                           <  1:83732/98‑1 (MQ=255)

                                                                                                                        cAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAGt  >  1:176266/1‑139 (MQ=255)

                                                                                                                        cAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAGt  >  1:663161/1‑139 (MQ=255)

                                                                                                                        cAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATGTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAGt  >  1:430059/1‑139 (MQ=255)

                                                                                                                           tGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGaa                  >  2:514285/1‑120 (MQ=255)

                                                                                                                           tGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGaa                  <  1:514285/120‑1 (MQ=255)

GAGATCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGCGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAGT  >  NC_000913/2218078‑2218336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A