Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,076,627	G→C	S161T (AGT→ACT)	yihY →	BrkB family putative transporter, inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,076,627	0	G	C	100.0%	277.4 / NA	77	S161T (AGT→ACT)	yihY	BrkB family putative transporter, inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (39/38); total (39/38)

GATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTCCTACCATCCGCGTAC  >  NC_000913/4076493‑4076761

gATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTcc                                                                                                                                    >  1:961815/1‑139 (MQ=255)

     aCTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTAttt                                                                                                                               >  1:533888/1‑139 (MQ=255)

      cTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTg                                                                                                                              >  2:101380/1‑139 (MQ=255)

       tCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGc                                                                                                                             <  2:616870/139‑1 (MQ=255)

       tCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGc                                                                                                                             <  2:1110881/139‑1 (MQ=255)

        ccATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGct                                                                                                                            >  1:934767/1‑139 (MQ=255)

        ccATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGc                                                                                                                             >  2:144434/1‑138 (MQ=255)

        ccATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGc                                                                                                                             <  1:144434/138‑1 (MQ=255)

        ccATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCACTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGct                                                                                                                            >  2:294102/1‑139 (MQ=255)

           tCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCtctc                                                                                                                         >  2:1135210/1‑139 (MQ=255)

             gATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTccc                                                                                                                       <  2:357502/139‑1 (MQ=255)

                 gcgcGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTgcg                                                                                                                   <  2:961815/139‑1 (MQ=255)

                 gcgcGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTgcg                                                                                                                   >  2:264409/1‑139 (MQ=255)

                  cgcgTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTgcgc                                                                                                                  >  2:996752/1‑139 (MQ=255)

                  cgcgTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTgcgc                                                                                                                  >  2:382273/1‑139 (MQ=255)

                   gcgTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCt                                                                                                                 >  2:405798/1‑139 (MQ=255)

                    cgTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTg                                                                                                                >  2:8619/1‑139 (MQ=255)

                         aaTACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGgcga                                                                                                           <  1:101380/139‑1 (MQ=255)

                           tACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGgcgagc                                                                                                         <  2:628886/139‑1 (MQ=255)

                           tACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGgcgagc                                                                                                         <  2:457435/139‑1 (MQ=255)

                              cATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGAt                                                                                                      <  2:934767/139‑1 (MQ=255)

                              cATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGAt                                                                                                      <  2:21991/139‑1 (MQ=255)

                               aTCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGAtc                                                                                                     >  2:759822/1‑139 (MQ=255)

                                tCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTggg                                                                                                              >  2:1060150/1‑129 (MQ=255)

                                tCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTggg                                                                                                              <  1:1060150/129‑1 (MQ=255)

                                tCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGAtct                                                                                                    <  2:533888/139‑1 (MQ=255)

                                   ggCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCaa                                                                                                 >  1:679131/1‑139 (MQ=255)

                                   ggCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCaa                                                                                                 >  2:431571/1‑139 (MQ=255)

                                     cgcAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATa                                                                                               <  1:996752/139‑1 (MQ=255)

                                       cAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACt                                                                                             <  1:8619/139‑1 (MQ=255)

                                           aaaCGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGc                                                                                                                             >  2:315690/1‑103 (MQ=255)

                                           aaaCGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGc                                                                                                                             <  1:315690/103‑1 (MQ=255)

                                            aaCGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCAt                                                                                        <  2:905565/139‑1 (MQ=255)

                                                    cgACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACa                                                                                   >  1:266982/1‑136 (MQ=255)

                                                    cgACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACa                                                                                   <  2:266982/136‑1 (MQ=255)

                                                    cgACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACg                                                                                >  2:231983/1‑139 (MQ=255)

                                                    cgACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACg                                                                                >  1:638294/1‑139 (MQ=255)

                                                      aCCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGc                                                                                                                             <  1:915199/92‑1 (MQ=255)

                                                      aCCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGAGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCAACAACGtg                                                                              >  2:812282/1‑139 (MQ=255)

                                                      aCCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTCTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGc                                                                                                                             >  2:915199/1‑92 (MQ=255)

                                                       ccAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGtgc                                                                             <  1:1016171/139‑1 (MQ=255)

                                                                     gTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCg                                                               <  1:1135210/139‑1 (MQ=255)

                                                                                    gatgatTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACtt                                                                                                                                      >  2:1150511/1‑53 (MQ=255)

                                                                                      tgatTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGAt                                              <  1:657598/139‑1 (MQ=255)

                                                                                       gatTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGc                                                                                                                             <  1:106690/59‑1 (MQ=255)

                                                                                       gatTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGc                                                                                                                             >  2:106690/1‑59 (MQ=255)

                                                                                         ttttAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGAtctc                                           >  2:119760/1‑139 (MQ=255)

                                                                                         ttttAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGAtctc                                           >  1:1033344/1‑139 (MQ=255)

                                                                                         ttttAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGAtctc                                           >  1:181264/1‑139 (MQ=255)

                                                                                         ttttAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGAtctc                                           >  2:972057/1‑139 (MQ=255)

                                                                                                 cTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGt                                   <  1:382273/139‑1 (MQ=255)

                                                                                                 cTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGt                                   <  1:972057/139‑1 (MQ=255)

                                                                                                  tGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGgcgag                                                                                                          >  1:1061135/1‑67 (MQ=255)

                                                                                                  tGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGgcgag                                                                                                          <  2:1061135/67‑1 (MQ=255)

                                                                                                        cGCTGCTGGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTCCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTa                            <  1:812282/139‑1 (MQ=255)

                                                                                                             ctgGCAGGGGCCAGTCTGTCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCa                       <  2:1033344/139‑1 (MQ=255)

                                                                                                             ctgGCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCa                       <  1:119760/139‑1 (MQ=255)

                                                                                                               ggCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCAtt                     >  2:564926/1‑139 (MQ=255)

                                                                                                               ggCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCAtt                     >  2:423199/1‑139 (MQ=255)

                                                                                                                gCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGtt                                                             >  2:494745/1‑98 (MQ=255)

                                                                                                                gCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGtt                                                             <  1:494745/98‑1 (MQ=255)

                                                                                                                gCAGGGGCCAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTg                    <  1:264409/139‑1 (MQ=255)

                                                                                                                       ccAGTCTGGCGATCACTTCCTATTTGCTCTCCCTgcg                                                                                                                   <  1:3603/37‑1 (MQ=255)

                                                                                                                       ccAGTCTGGCGATCACTTCCTATTTGCTCTCCCTgcg                                                                                                                   >  2:3603/1‑37 (MQ=255)

                                                                                                                       ccAGTCTGGCGATCACTTCCTATTTGCTCTCCCTgcg                                                                                                                   >  1:1269499/1‑37 (MQ=255)

                                                                                                                       ccAGTCTGGCGATCACTTCCTATTTGCTCTCCCTgcg                                                                                                                   <  2:1269499/37‑1 (MQ=255)

                                                                                                                       ccAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGt                                   >  2:496131/1‑117 (MQ=255)

                                                                                                                       ccAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGt                                   <  1:496131/117‑1 (MQ=255)

                                                                                                                       ccAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTCCTAc             <  1:405798/139‑1 (MQ=255)

                                                                                                                        cAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTCCTAcc            <  2:441712/139‑1 (MQ=255)

                                                                                                                        cAGTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTCCTAcc            <  1:164467/139‑1 (MQ=255)

                                                                                                                          gTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATa                                                                                               >  1:152583/1‑54 (MQ=255)

                                                                                                                          gTCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATa                                                                                               <  2:152583/54‑1 (MQ=255)

                                                                                                                           tCTGGCGATCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTCCTACCATc         >  2:792228/1‑139 (MQ=255)

                                                                                                                              ggCGATCACTTCCTATTTGCTCTCCCTGCGCTGGgcgagc                                                                                                         <  1:436908/40‑1 (MQ=255)

                                                                                                                              ggCGATCACTTCCTATTTGCTCTCCCTGCGCTGGgcgagc                                                                                                         >  2:436908/1‑40 (MQ=255)

                                                                                                                                  aTCACTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTCCTACCATCCGCGTAc  >  2:268643/1‑139 (MQ=255)

GATGTACTCCATCGATAGCGCGTTGAATACCATCTGGCGCAGTAAACGAGCGCGACCAAAAATTTACTCGTTCGCCGTGTACTGGATGATTTTAACGCTGGGGCCGCTGCTGGCAGGGGCCAGTCTGGCGATCAGTTCCTATTTGCTCTCCCTGCGCTGGGCGAGCGATCTCAATACTGTCATCGACAACGTGCTGCGTATTTTTCCGTTGCTGTTGTCGTGGATCTCCTTCTGGTTGCTGTACAGCATTGTTCCTACCATCCGCGTAC  >  NC_000913/4076493‑4076761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A